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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,030 results for: URE

Showing: 21 - 40 of 1030 results

  1. Deisseroth INTRSECT Collection

    Type
    Collection
    ...target populations defined by multiple parameters. Figure 1: Examples of intersectional cell population definitions...expanded to incorporate VCre in order to enable three-feature cell targeting. C) Example of viral targeting of...viruses delivering additional recombinases 10–14 . Figure 2: INTRSECT works by inserting short, intronic ...Deisseroth Lab maintains a Standard Operating Procedure (Link opens in a new window) with general principles...Targeting cells with single vectors using multiple-feature Boolean logic. Nat Methods 11(7):763-72. PubMed...fear-promoting circuit in the extended amygdala. Nature 537(7618):97-101. PubMed (Link opens in a new window...2016. Midbrain circuits for defensive behaviour. Nature 534(7606):206-12. PubMed (Link opens in a new window...

  2. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...AAV BsaI yes, cut S. aureus Zhang pX602 61593 Mammalian/AAV BsaI yes, cut S. aureus Zhang pSaGuide 64710...need plasmid 65776 S. aureus Joung BPK2101 65770 Bacteria BsmBI yes, cut S. aureus Joung Multiplex gRNA...designed to be used with, such as S. pyogenes, S. aureus, N. meningitidis, etc. gRNA scaffolds are specific...Crimson Bauer BPK2660 70709 Mammalian U6 none S. aureus Joung pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro 71236...64710 Mammalian BbsI none S. aureus Musunuru BPK2301 65778 Mammalian BsmBI none, need plasmid 65775 N. meningitidis...Editing in Monocot and Dicot Plants Plant PCR-based procedure to rapidly generate multiple sgRNA expression ...

  3. Tetracycline Inducible Expression

    Type
    Collection
    ...tetracycline and releases tet O, enabling transcription (Figure 1). While TetR and tet O could be a basic tool ...synthetic Tet Response Element (TRE) typically features seven copies of tet O upstream of a minimal CMV...or skip ahead to view highlighted Tet plasmids . Figure 1: Tet-regulated expression systems. Left: natural..., although the DNA-binding profile is reversed (Figure 1). However, rtTA can induce stronger expression...vector design, but it is easier to implement and ensures that all transfected or transduced cells express...can be found in Krueger et al., 2004. Some cell culture sera may contain tetracycline or its derivatives...can result in unintended induction. Check the manufacturer’s details to know whether your serum is tetracycline-free...

  4. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ... the orientation of each target site (see Figure 1). Figure 1: Recombination outcomes are determined by...site (called loxP for Cre, or FRT for Flp). The nature of the DNA rearrangement after recombination (inversion...systems. Enzyme Target Site Canonical Sequence Structure (Length) Common Variants Cre loxP ATAACTTCGTATAgcatacatTATACGAAGTTAT...recombinases. Many of our in-stock viral preps feature Cre-dependent tools, or consider our AAV Packaged...recombinases. Many of our in-stock viral preps feature Flp-dependent tools, or consider our AAV Packaged...

  5. Rett Syndrome

    Type
    Collection
    ...curated literature information (Link opens in a new window) OMIM MECP2 - MECP2 curated literature information...2008 to drive the development of treatments and cures for Rett Syndrome and related MECP2 disorders. The...delay between 6 and 18 months of age. A defining feature, with onset typically between 18 to 30 months, ...InterRett - international online database of clinical features and genetic characteristics of Rett syndrome (...Rett syndrome: revised diagnostic criteria and nomenclature. Ann Neurol . 68, 944–950. PMID: 21154482 Neul...

  6. Neurodegeneration Research Collection

    Type
    Collection
    ...are no effective therapies to prevent, treat, or cure most of these disorders. An integrated study of ...people over the age of 60. There is presently no cure or way to prevent progression of PD. Treatment consists...the idiopathic (having no specific known cause) nature of the disease, a small portion of cases can be... receptor (DTR)–GFP fusion protein. Azim et al. Nature. 2014 Apr 17. Next-generation GRAB sensors for ... new window) A foundation dedicated to finding a cure for Parkinson's disease through an aggressively ...

  7. COVID-19 Resources

    Type
    Collection
    ... one-enzyme RT-qPCR assay for SARS-CoV-2, and procedures for reagent production. Bhadra S, et al. bioRxiv...Transcriptase plasmids from Andrew Ellington's lab. High-surety isothermal amplification and detection of SARS-...Science Journals Coronavirus Collection Springer Nature SARS-CoV-2 and COVID-19 Articles Wiley Online Library...Kit References ACE2 - Andersen, KG, et al, 2020, Nature Medicine The proximal origin of SARS-CoV-2 . TMPRSS2... Inhibitor . FURIN - Andersen, KG, et al, 2020, Nature Medicine The proximal origin of SARS-CoV-2 . BSG...

  8. Genetic Code Expansion

    Type
    Collection
    ... you will need two plasmids, as depicted in the figure below: A plasmid expressing the tRNA and its cognate...amino acids within a protein, any alterations in structure or function in the protein can be observed. The...working off of a previously established protocol, make sure to match the growth medium, ncAA concentration, ...codon. Therefore many controls must be used to make sure that these conditions are true. Always express first...protein with and without the ncAA in the media to make sure that the full length protein is only made when the...using genetic code expansion without buildup of prematurely truncated protein. Ryan Mehl 197656 BL21(DE3)...

  9. Immunology Research Plasmids and Resources

    Type
    Collection
    ...MGC129975, UI, UROC UCN2 urocortin 2 SRP, UCN-II, UCNI, UR, URP UCN3 urocortin 3 (stresscopin) MGC119002, SCP...FMRFAL NPPA natriuretic peptide precursor A ANF, ANP, ATFB6, CDD-ANF, PND NPPB natriuretic peptide precursor...precursor B BNP NPPC natriuretic peptide precursor C CNP NPR1 natriuretic peptide receptor A/guanylate ...cyclase A (atrionatriuretic peptide receptor A) ANPRA, ANPa, GUC2A, GUCY2A, NPRA NPR3 natriuretic peptide...NR61, RTR NRG1 neuregulin 1 ARIA, GGF, GGF2, HGL, HRG, HRG1, HRGA, NDF, SMDF NRG2 neuregulin 2 Don-1, HRG2...HRG2, NTAK NRG3 neuregulin 3 HRG3, pro-NRG3 NRG4 neuregulin 4 DKFZp779N0541, DKFZp779N1944, HRG4 NRP1 neuropilin...response is triggered within the first few minutes of exposure to a pathogen and is the dominant defense strategy...

  10. Antibody Production

    Type
    Collection
    ...with Coomassie blue. Protein band intensities are measured using ImageJ software and a standard curve is ...intensities of both the antibody and impurities are measured and the ratio of the antibody protein content ...antibody or the hybridoma-derived equivalent to ensure that potency and specificity have been retained...passed stability testing, it may ship at room temperature. However, Addgene determines ideal shipping conditions...

  11. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...endogenously-tagged cellular structures. Addgene ID Plasmid Allen Institute ID Tag Protein Structure 87420 PXN-EGFP ...fluorescently tagged proteins of key cellular structures in human cell lines. Depositor...fluorescently tagged proteins of key cellular structures in human cell lines of their choice. For a full...

  12. AAV Packaged on Request

    Type
    Collection
    ...amplification, and high-quality quality control measures such as titering, purity assessment, sterility... comprehensive quality control processes to make sure it is ready for your experiments. Shipping 2–7 days...globally connected scientific community. The Data Hub captures valuable knowledge that often goes unshared, bridging...including purification, titration, and quality control measures. We have distributed preps around the globe and...

  13. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...This new resource described in (Gassaway et al., Nature Methods 2022) is adapted from the synthetic human...Haimovich AD, Rogulina S, Isaacs FJ, and Rinehart J. Nature Communications . 2015. 6, 8130. PubMed (Link opens... Haar CM, Isaacs FJ, Rinehart J, and Jewett MC. Nature Communications . 2015. 6, 8168. PubMed (Link opens... TBK1 Activation to Promote Mitophagy. Heo JM, Ordureau A, Paulo JA, Rinehart J, Harper JW. Molecular ...

  14. TALEN Plasmids and Kits

    Type
    Collection
    ...and 63AA, respectfully). This next generation architecture has been shown to increase mutation induction... enables the evaluation of TALEN activities in cultured cells. Golden Gate TALEN 2.0 47388 pcDNA3.1-GoldenGate...systems including hard-to-transfect cell lines. Features include (i) Esp3I (BsmBI) restriction sites for...cells, (iv) an improved, truncated TALE backbone architecture as established by Miller et al. (PMID: 21179091...

  15. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...are primarily used for determining protein 3D structure and crystallography.... that aims to determine the three dimensional structures of proteins of medical relevance, and place them...empty backbones/controls): ID Plasmid GenBank Key Features 26092 p15TV-L EF456736 Hexahistidine tag with ...

  16. CRISPR Plasmids and Resources

    Type
    Collection
    ...Addgene works with leading scientists in the field to ensure our CRISPR collections and resources are up-to-...below by function. Addgene's CRISPR collections feature plasmids for a variety of organisms, including ...fused to an epigenetic modifier alters chromatin structure/gene expression. RNA Targeting RNA Targeting Type...

  17. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...order. Experimental Disclaimer Due to the novel nature of experiments carried out with our materials, ...function of any viral vector. Addgene performs QC measures which verify the identity of the viral vector,...thorough shipping documents and following import procedure, it is the responsibility of the importer to pay...

  18. Synthetic Biology - Overview

    Type
    Collection
    ...biology resources available at Addgene. Browse featured collections or depositing labs....Bacterial Plant Yeast Mammalian Worm Fungal Algal Featured SynBio Deposits Alper Lab Y. Lipolytica Promoters...papers on Syn Bio SEVA - Standard European Vector Architecture Open Plant - Plant SynBio resource Synthetic...

  19. Antibody Plasmid Collection

    Type
    Collection
    ... into mammalian tissue culture cells, the R-mAbs are secreted into the culture media and can be collected...Plasmids The table below highlights plasmids that feature expression of antibodies, nanobodies or ScFvs. ...

  20. Promega Plasmid Collection

    Type
    Collection
    ...use light produced from an enzymatic reaction to measure subtle expression changes with high sensitivity...window) is an energy transfer technique designed to measure molecular proximity in living cells. NanoBRET PPI...protein. The apparent affinity of test compounds is measured by competitive displacement of a NanoBRET tracer...
Showing: 21 - 40 of 1030 results