28 Hot Plasmid Technologies from 2015
Type
Blog Post
... Cas9 into n-terminal (Cas9(N)-2xNLS) and c-terminal (Cas9(C)-2xNLS) fragments facilitates target DNA ...activation, the c-terminal cas9 fragment was fused with FK 506 binding protein 12 (Cas9(C)-FKBP-2xNLS) and...expression vector which gives close to 100% cloning efficiency and gene expression. The pUCXKT vector ensures...different N- and C-terminal tags. Additionally, by using an adapted destination vector with a heterologous...science.aaa3380
MuLE vectors for customized lentivirus production
Finding a lentiviral vector backbone with ...custom-cloned vectors in a Multisite Gateway reaction with a desired destination vector expressing a separate...a final lentiviral vector containing up to 5 different elements.
Lentiviral vectors constructed with the...