We narrowed to 324 results for: cloning

Protocol for Ligation Independent Cloning

Protocols for plasmid cloning by PCR.

Addgene's protocol for using the pLKO.1 cloning vector, a backbone used by the RNAi consortium for targeting human and mouse genes.

Modify your plasmid MCS or add short sequences using oligo overlap cloning.

A wide variety of protocols from Addgene that can be used for basic molecular biology, plasmid cloning, and titering and testing your viral preparations.

Information about plasmid cloning by restriction enzyme digest (subcloning), including design and experimental procedures.

Learn about different molecular cloning techniques, focusing on seven common cloning methods.

Learn about modular cloning (MoClo) and find MoClo kits deposited with Addgene, including the MoClo Toolkit and the MoClo Plant Parts Kit.

Learn how you can use MXS-Chaining to generate complex, multicistronic plasmid constructs.

Learn how to use and apply NEBuilder HiFi DNA Assembly Technology from New England Biolabs for quick and accurate plasmid cloning.

Colony PCR is a useful technique for quickly screening for successful constructs after a plasmid cloning experiment.

Learn more about the common E. coli strains used in the lab.

Using plasmids for transformation, cloning, or transfection? Different uses mean you'll need different purities of DNA for your applications.

Blue-white screening is a well loved technique that allows scientists to quickly identify recombinant clones for further analysis.

Fragmid is a quick, easy way to design and create CRISPR plasmids. Read about the Doench lab's new modular cloning-based approach in this blog post!

A brief summary of the history of plasmids and their use in cloning and molecular biology.

A list of free online molecular biology tools for plasmid mapping, DNA and protein sequence analysis, primer design and more.

GreenGate is a cloning system for rapidly assembling plant transformation constructs and is based on the Golden Gate method.

New to plasmids? Learn the basics of plasmids in this blog post and find out about the parts of a plasmid, how they are made, and how scientists can use them in the lab.

22 of the interesting and popular hot plasmid technologies of 2014 from Addgene's plasmid repository.