We narrowed to 229 results for: cre encoding plasmid

... usage bias in Plasmids 101: Codon usage bias Read more about plasmids in our Plasmids 101 blog series...that encode 20 amino acids and three stop codons, meaning that the same amino acid can be encoded by more...this variation in tRNA expression, the codons that encode a protein may affect the rate of translation and...(A) The codon wheel depicts the 64 codons that encodes the 20 amino acids and three stop codons. (B) Example...different organisms. There are several codons that encode the same amino acid. Each organism, however, may...addition codon optimization could remove information encoded in the primary sequence of a particular gene which...Reference Read about genetic code expansion Browse our plasmid collection page  ...

...collections of plasmids as kits with greatly reduced costs per plasmid. We generally create kits to allow...just the tool you need. The plasmids in this portion of the BIOFAB Plasmid Set contain terminators of ...populations. This kit consists of a 48 plasmid adapter library, with each plasmid carrying a unique barcoded Illumina-M13F... selection markers built into all plasmids in the kit help decrease false positives observed in the cloning...library by in vivo functional screen." Scientific reports 5 (2015). BIOFAB plasmid set 3000 Series Confronted...researchers at the BIOFAB and the Endy Lab created this kit. The plasmids within it contain a variety of different...researcher at a nonprofit institution, you can order plasmids covering a wide range of fields from Addgene. ...

...known in your lab. Plasmid Type Choose from: Encodes one insert, encodes gRNA/shRNA, or empty backbone...deposits with more than 10 plasmids is to use our deposit spreadsheet?  Our plasmid deposit process can be... Send plasmid information to Addgene Material Transfer Agreement (MTA) approval  Send plasmids to Addgene...spreadsheet and copy and paste your plasmid data directly into our file, one plasmid per row, as outlined by the... Data Field Description Plasmid Name Use a descriptive plasmid name and match the name as it ...application of the plasmid and will help a scientist outside your field understand if this plasmid will work for... Enter bacterial antibiotic resistance encoded in the plasmid that should be used to maintain a glycerol...

...Addgene-Seeding Labs plasmid grant is a collaborative endeavor providing plasmids to researchers in developing...genetically encoded H2O2 probe is pQE-30-HyPer3 (2) which he will co-express together with a plasmid encoding...Addgene-Seeding Labs Plasmid Grant Check Out Previous Addgene-Seeding Labs Plasmid Grant Awardees Listen... vector and the pOKD plasmids. The recombinant Cry proteins will then be screened for toxicity against...scientists who were awarded the Addgene-Seeding Labs Plasmid grant this year. These scientists work on projects...excited to see how all of the Addgene-Seeding Labs Plasmid Grant awardees' projects progress. Subscribe to...Bilan, Dmitry S. et al. “HyPer-3: A Genetically Encoded H2O2 Probe with Improved Performance for Ratiometric...

...engineering efforts have increased the brightness of these genetically encoded light emitters (i.e. Takai...the most popular, most widely used genetically encoded fluorescent probe. Several factors contribute to... the first choice when selecting a genetically encoded fluorescent tag. There are, however, a number of...may alter diffusion rates. Smaller genetically encoded probes are based on cofactor binding proteins as...Both systems are a hybrid between a genetically encoded tag and exogenously added components. For a review...date, small probes that are entirely genetically encoded do not exist, but the development of non-natural...are made by connecting its DNA code to the cDNA encoding for another protein. Effectively, it equips the...

...our control plasmids in new serotypes, as well as a new Cre dependent GFP control plasmid for expression...development of future therapies. Our popular plasmid encoding a Cre dependent version of the DREADD hM3D is...are now available as AAV1. CRE-DOG constructs 69570 and 69571 together encode the N and C terminal segments...dependent Cre. The two segments come together in the presence of GFP to form a functional Cre enzyme that...both Cre and GFP. The GFP dependent FLPo, Flp-DOG (75469), is also available in AAV1. Plasmid Serotype...ChR2 for targeted expression DREADDs GFP dependent Cre and FLPo And more!  New lentiviral preps SARS-...protein expression The Krogan lab quickly shared plasmids expressing SARS-CoV-2 proteins at the beginning...

... involving two-plasmid or single-plasmid systems (Vo et al., 2020). In the two-plasmid system, an effector...promoter, and a donor plasmid (pDonor) encodes the mini-transposon. pEffector and pDonor can also be combined... mini-transposon Transform plasmid(s) into cells and plate overnight Screen colonies phenotypically or...(V. cholerae INTEGRATE - hereafter VchINT) and encodes a Type I-F CRISPR-Cas system. The four major components...group’s initial report of VchINT, three separate plasmids and multiple promoters were used to express the...effector plasmid (pEffector) expresses all seven required genes and the CRISPR RNA under the same promoter...combined in single-plasmid INTEGRATE (pSPIN), which offer simple transformations for smaller cargos, and ...

...) Interested in More Hot Plasmids from Addgene? Check out our Hot Plasmid page, where we highlight some...popular & newly available plasmids at Addgene. If you're interested in plasmids for in vivo imaging, check...for Neurobiology has been working on genetically encoded indicators of calcium and other small molecules...the greatest challenges of neuroscience.”  Hot plasmids: Twitch sensors Griesbeck’s recent contribution... including his zinc sensor and his genetically encoded magnesium sensor, the first of its kind. “I hope...Lindenburg et al. MagFRET: The First Genetically Encoded Fluorescent Mg(2+) Sensor. PLoS One. 2013 Dec 2...large-scale functional screen in bacteria. Sensor variants were then refined by a secondary screen in rat hippocampal...

...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...ChiP-seq. Learn more about ENCODE Find the transcription factor tagging plasmids If you have a new CRISPR...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...Read the paper in Science Find the new SHERLOCK plasmids More precision with dual-nuclease Cas9-Cas9 ...Nature Communications Find the Cas9-Cas9 chimera plasmids A modular toolkit for genome editing in plants...post Read the paper in Science Find the Cas14 plasmids Anti-CRISPR proteins for optogenetic control...paper in Nature Methods Find the anti-CRISPR plasmids Tagging human transcription factors with GFP...

...control AAV encoding GFP. Check out our inventory of serotype testing AAV New control AAVs Plasmid Serotype...Chemogenetics Plasmid Serotype Name 119472  AAV5  AV SYN PSAM4 GlyR IRES EGFP   Biosensors Plasmid Serotype...biosensor AAV inventory New glutamate sensors Plasmid Serotype Name 106176  AAV1  pAAV.hSynapsin.SF-iGluSnFR.S72A...pAAV.hSynapsin.SF-iGluSnFR.S72A New calcium sensors Plasmid Serotype Name 104496  AAV1 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE...pAAV-hSynapsin1-FLEx-axon-GCaMP6s New voltage sensors Plasmid Serotype Name 119036  AAV1  pAAV-hsyn-flex-Voltron-ST...Sternson lab recently deposited PSAM AAV, which encode engineered ligand-gated ion channels that can be...of DREADDs / Chemogenetics AAVs New DREADDs Plasmid Serotype Name 75033  AAV1 pAAV CD68-hM4D(Gi)-...

...combines the Cre/lox system with optogenetics. Cre activity is controlled by red light by splitting Cre and fusing... Figure 4: The C-terminus of Cre (CreC) and the N-terminus of Cre (CreN) are fused to the truncated Phytochrome... previous Cre/lox or drug-induced Cre systems (ex: tamoxifen). As the name suggests, CreLite combines ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...genetically encoded voltage indicator Article contributed by Shreya Vedantam Genetically encoded voltage ... dimerize, bringing each terminus of Cre together to restore Cre activity. As soon as red light or PCB...

... are not encoded by the transfer plasmid, but instead are provided in trans by other plasmids or the packaging...components are needed: γ-Retroviral transfer plasmid encoding a transgene, sgRNA, or shRNA of interest: ...γ-retroviral packaging plasmids are not interchangeable. General envelope plasmids, such as VSV-G (Vesicular...in trans by specific plasmids. This method, therefore, reduces the number of plasmids that are required ...γ-retroviruses self-inactivating (SIN)? They can be. Plasmids that encode SIN γ-retrovirus lack most of the U3 region...do many γ-retroviral plasmids have similar names? The nomenclature of the plasmid backbone is meant to..., which facilitate integration of the transfer plasmid sequences into the host genome. Typically, it is...

...deposited the resulting optogenetic plasmid tools which includes plasmids encoding a fusion between cPYP and tgRFP...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need... recent plasmid deposit please sign up here.          Listen to this episode of Hot Plasmids! Optogenetic...Grefen and Blatt, 2012). The 2in1 Plasmid Toolkit consists of 4 plasmid toolkits generated with 4 different... tgRFP (PLL7.0 tgRFP cPYP), and plasmids that encode the protein binders (which bind to either the light...targeting and editing experiments. Find the CASANOVA plasmids here! Bubeck F et al., Nature Methods 2018. PubMed...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need... our hot plasmid articles from April 2018. If you'd ever like to write about a recent plasmid deposit ... from the El-Samad lab recently deposited plasmids encoding synthetic yeast transcription factors that...activation of gene expression. In addition to plasmids encoding these synthetic transcription factors, the...the El-Samad lab has deposited plasmids encoding fluorescent reporters and other proteins controlled by...sign up here. No time to read? Listen to our Hot Plasmids Podcast! TRIM-Away: Targeted endogenous protein...

... New England Biolabs Cell-Imaging Plasmid Collection Promega Plasmid Collection ..., Cas9-PCV HUH-tag Cas9 plasmids with N- or C-terminal PCV2 HUH-tag; increase homology-directed repair... covalent bond formation between a genetically encoded peptide (SpyTag) and protein (SpyCatcher) SnoopTag... covalent bond formation between a genetically encoded peptide (SnoopTag) and protein (SnoopCatcher); ... covalent bond formation between a genetically encoded peptide (DogTag) and protein (DogCatcher); ideal...NEB Cell-Imaging Collection SNAP-tag, CLIP-tag Plasmids for visualization of proteins in live or fixed...entry clone to add C-terminal SNAP-tag Promega Plasmid Collection HaloTag Collection of research tools...

...is split into two plasmids: one encoding Rev and one encoding Gag and Pol, increasing the number of recombination...consisted of three plasmids: the packaging, envelope, and transfer plasmids. The packaging plasmid carried a mutated...vector could infect. Finally, the transfer plasmid encoded the desired transgene flanked by HIV-1 long... packaging and envelope plasmids are two of Addgene’s most requested plasmids! Lentiviral particles with...Many CRISPR plasmids are designed for lentiviral use, including Addgene’s top requested plasmid in 2015: ... Lentiviral Plasmid FAQs. Lentiviral vectors in the lab Lentiviral vectors are incredibly popular in the...itself due to a few missing proteins. The envelope plasmid contained a viral envelope to dictate the tropism...

...on the plate. For example, if your plasmid contains a gene encoding a blue chromoprotein, after transforming...colonies to determine which cells have taken up the plasmid. Of course, other follow up methods like sequencing... chromoprotein sequences into E. coli BioBrick plasmids (meffRed, eforRed, asPink, spisPink, scOrange,...chromoproteins. In some cases, cells containing high-copy plasmid-borne chromoproteins lost color overnight in liquid...problem. There is no single “ideal” chromoprotein plasmid that combines the most desirable features: intense...Chromoproteins for allelic exchange Figure 3: pTOX plasmids expressing amilCP and tsPurple genes. Image: Lazarus...sequential homologous recombination events: The plasmid carrying the desired genetic modification integrates...

...virus (MoMLV) and consisted of two plasmids: the packaging plasmid containing all the structural genes...Gag, Pol, Tat, Rev and Env) and the transfer plasmid encoding the insert of interest as well as sequences...Psi). Because the packaging plasmids lacked the psi signal, the genes encoding the structural proteins could...crossover event between the vector plasmid and the packaging plasmid caused the Psi sequence to be placed...lentiviral vectors consisted of three plasmids encoding 1) the lentiviral vector genome which was composed...expression of the transgene   Transfer plasmids The transfer plasmid is the vector you clone your gene of...usually 293T cells, with your transfer plasmid and your packaging plasmids. For this, you can use the transfection...

...instance, Lifeact-mTurquoise2 (Plasmid #36201) or mScarlet-tubulin (Plasmid #85045). We often use restriction... organisms. Both components of the chimera are encoded by DNA. Since researchers can generate almost any...Aequorea victoria and its variants are genetically encoded fluorescent probes. One of the limitations is the...over-expression artifacts The introduction of DNA that encodes a fusion protein adds protein to the existing pool...advisable to first express the fusion protein from a plasmid-based system to assess whether the chimera is still... develops, characterizes, and uses genetically encoded fluorescent probes. You can follow him on twitter...Download the fluorescent proteins 101 eBook Read our plasmids 101 blog series Resources on Addgene.org Browse...

...labeling strategies have two parts: (1) a genetically encoded “tag” expressed as a fusion with your favorite ...ligand (e.g., FlAsH, ReAsH) and a short genetically encoded tetracysteine (Cys4) peptide tag could be used ...These widely used systems consist of a genetically encoded enzyme variant tag that reacts specifically and...paclitaxel. Unlike other systems, no genetically encoded tag is required as the small-molecule binding motif...trade-offs between the size of the genetically encoded tag, the speed and selectivity of the fluorophore... Generate Fluorescent Protein Fusions Request Plasmids from the Michael Davidson Collection Find Fluorescent...overexpression of protein fusions to gene-edited cells can decrease the number of fluorophores, compromising the photon...