...system (Figure 9C) SAM activators - co-expression of dCas9-VP64 with a modified scaffold gRNA and additional...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects...achieved by using orthogonal dCas9s (e.g. S. pyogenes dCas9 and S. aureus dCas9) tagged with different fluorescent...biotin tagging of dCas9 by fusing a biotin acceptor site to dCas9 and co-expressing BirA biotin ligase...multiple gRNAs using a single plasmid ensures that all gRNAs will be expressed in the same cell. This is ...genomic DNA. dCas9 has also been used for genome-wide screens to activate or repress gene expression in mice...human cells. The simplest dCas9-based activators and repressors consist of dCas9 fused directly to a single...

...Mammalian Lentiviral expression of Cas9, dCas9, or dCas9-VP64 along with 1-4 sgRNAs expressed from independent... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does...Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut...maintained by the Doudna Lab . Plasmid Expression System Cloning Enzyme Co-expressed Cas9 Cas9 System Selection...can be used to express multiple gRNAs from a single plasmid with or without expression of Cas9. Highlighted.... Can express Cas9 with up to 3 gRNAs. Frew pSQT1313 Mammalian Single plasmid for the expression of multipe...requires expression of Csy4 (eg Plasmid 53369 ). Joung pX333 Mammalian Single all-in-one plasmid for tandem...

...system (Figure 9C) SAM activators - co-expression of dCas9-VP64 with a modified scaffold gRNA and additional...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects...achieved by using orthogonal dCas9s (e.g. S. pyogenes dCas9 and S. aureus dCas9) tagged with different fluorescent...biotin tagging of dCas9 by fusing a biotin acceptor site to dCas9 and co-expressing BirA biotin ligase...multiple gRNAs using a single plasmid ensures that all gRNAs will be expressed in the same cell. This is ...genomic DNA. dCas9 has also been used for genome-wide screens to activate or repress gene expression in mice...human cells. The simplest dCas9-based activators and repressors consist of dCas9 fused directly to a single...