We narrowed to 28 results for: dna oligonucleotides

...flanking the DNA-binding cleft of Cas9, the fluorescent domains dimerize and prevent DNA binding in the... cell, the dCas9-CIB1 fusion binds to the target DNA sequence as directed by the guide RNA (gRNA), while...unveiled a photoactivatable system to cleave a target DNA sequence (Nihongaki, et al., 2015) resulting in a... Additionally, high intensity exposure can cause DNA damage. Far-red light, however, can penetrate 5 mm...fluorescent reporter gene in mice using Minicircle DNA vectors encoding the FAST constructs. This proof-...stimulation, the pdDronpa moieties move apart, allowing DNA binding and cleavage. This technique was applied ...deactivated the protein’s ability to cleave targeted DNA, settling on photocaging the K866 lysine, as seen...

...archaea, cleave double-stranded DNA (dsDNA) at specific sequence sites in the DNA. In restriction cloning, scientists...ends in the DNA fragments. Given its prevalence, the vast majority of plasmids used for DNA cloning and...flanking your DNA fragment of interest. Now that you have made an entry vector containing your DNA of interest... to 5' exonuclease activity of T4 DNA polymerase. In LIC, the T4 DNA polymerase’s exonuclease activity... has difficulty assembling DNA fragments with repetitive sequences and DNA that ends in sequences that...into yeast the fragments of DNA to be fused along with custom synthesized DNA oligos that span each junction...is the ability to perform oligonucleotide stitching, in which pieces of DNA that share no homology ends...

...simultaneously incorporate multiple single-strand DNA (ssDNA) oligonucleotides (oligos), and thereby rapidly create...of recombinant DNA technology has allowed molecular biologists to fuse fragments of DNA together in a ... enables transient suppression of DNA repair during oligonucleotide integration, allowing MAGE in otherwise...sequence. Should one of these random stretches of DNA contain an enhancer, it will activate its own transcription...process alone involves much literature searching, DNA synthesis, and plasmid assembly. Luckily, researchers... platform for generating both simple and complex DNA constructs Have you ever wished you could design...Assembly method, which allows for easy assembly of DNA fragments by utilizing regions of sequence homology...

...QCascade DNA-targeting module with the crRNA, (3) three transposase proteins, and (4) the donor DNA. By using...machinery to promote homologous recombination of linear DNA fragments. Since recombineering does not contain ...blocking the elongation complex from binding the DNA or extending the transcript. Different Cas orthologues...could be further refined by the addition of a uracil DNA glycosylase inhibitor and degradation tag on the ... Then, pCRISPR is introduced along with an oligonucleotide repair template. Through recombineering, the...gRNA(s), and the repair template is supplied as a dsDNA fragment. Each round of editing takes two days, ...s41564-018-0327-z Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA (2013) Repurposing...

...stranded DNA template strands. The DNA polymerase is also stable enough to now bind to the primer DNA sequence...Plasmid Cloning by PCR Restriction Digest of Plasmid DNA DNA Ligation Background Information A standard Polymerase...which would denature DNA polymerases from other species. In addition to the template DNA and the Taq polymerase...several micrograms of target DNA from just a few nanograms of template DNA through several cycles of denaturation...this step enables the DNA polymerase to synthesize and elongate the new target DNA strand accurately and... are using the same template DNA for all your reactions, the Template DNA can be added to the master mix...the number of nanomoles of DNA times 10. For example, if your lyophilized DNA is 38.5nm, add 385µl of water...

...recognition sites that flank a DNA module in an inverse orientation, so that multiple DNA components can directionally...cloning kit simplifies the cloning process for complex DNA constructs, particular for those involving recombination...easier exchange and re-use of existing elements. DNA sequences for a desired component are cloned into... Single Strand Annealing (SSA) in the event of a DNA break. Next, the gRNA being tested is expressed along... coli and Salmonella typhimurium) by integrating DNA sequences either sequentially or simultaneously. ...nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells. (Guilinger et ... and BpiI/BbsI to efficiently assemble up to six DNA fragments at a time. This method (based on the Golden...

...purify specific regions of DNA, image DNA in live cells, and precisely edit DNA and RNA. The ease of generating...application, the donor DNA may be a single-stranded oligonucleotide, double-stranded linear DNA, or a double-stranded...-stranded DNA (dsDNA) plasmid. Small single-stranded DNA (ssDNA) oligos often work well for small edits...homology with the target DNA. Once the Cas9-gRNA complex binds a putative DNA target, the seed sequence...will begin to anneal to the target DNA. If the seed and target DNA sequences match, the gRNA will continue...strands of the target DNA. This results in a double-strand break (DSB) within the target DNA, located ∼3–4 nucleotides...of target DNA. Cas9n increases target specificity. Since two nickases targeting opposite DNA strands are...

...purify specific regions of DNA, image DNA in live cells, and precisely edit DNA and RNA. The ease of generating...application, the donor DNA may be a single-stranded oligonucleotide, double-stranded linear DNA, or a double-stranded...-stranded DNA (dsDNA) plasmid. Small single-stranded DNA (ssDNA) oligos often work well for small edits...homology with the target DNA. Once the Cas9-gRNA complex binds a putative DNA target, the seed sequence...will begin to anneal to the target DNA. If the seed and target DNA sequences match, the gRNA will continue...strands of the target DNA. This results in a double-strand break (DSB) within the target DNA, located ∼3–4 nucleotides...of target DNA. Cas9n increases target specificity. Since two nickases targeting opposite DNA strands are...