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The development of adenoviral vectors for the delivery of CRISPR/Cas9 expands genome engineering to primary cells.

Learn about the different ways you can introduce DNA or RNA into cells using methods such as transformation, transduction, conjugation, and transfection.

Learn about the csRNP epitope tagging method - a new and efficient way to tag endogenous genes using CRISPR.

Learn how to use Cas9 nickases for genome editing, including homology directed repair.

Mobile-CRISPRi is a set of modular CRISPRi tools that can be used in diverse bacteria from various environments. It has the advantage of stable genomic integration and is easily transferred via conjugation.

An open-source biosensor platform, TRUPATH, measures the association of G protein signal transducers using bioluminescence resonance energy transfer.

Resources to help improve precision genome editing through homology directed repair in experiments with CRISPR nucleases.

Learn the basics behind generating generating mouse models using CRISPR/Cas9.

Nanoblades are a CRISPR delivery tool. These Nanoblades are engineered MLV-like particles loaded with Cas9-gRNA ribonucleoproteins.

A new open source tool for CRISPR experiments, CrispyCrunch, has been developed by the Chan Zuckerberg Initiative to design batch gRNAs, templates, and sequencing primers for CRISPR + HDR experiments.

An introduction to transposons including Sleeping Beauty, piggyBac, and Tol2 and how they can be used in transposon mutagenesis screens, gene transfer, and creating transgenic animals.

Check out some great blog posts from Addgene Depositors.

Recent developments using CRISPR/Cas9 editing with C. elegans, focusing on the use of an embryonic lethal selection marker to select for knock-in.

Learn how to verify indels, deletions, and homology directed repair (HDR) events in your CRISPR modified cell lines.

INTEGRATE is a bacterial genome engineering system that uses CRISPR to direct transposition to targeted locations in the genome.

Browse Addgene’s curated list of CRISPR resources and references, including gRNA design tools, CRISPR FAQs, and community forums.

If you’re using CRISPR to make a genome edit, how do you know if your experiment was successful? Try using fluorescent CRISPR reporters like SRIRACCHA and GEmCherry2.

We provide high quality AAV preps from select plasmids in the repository. Browse the University of Florida serotype testing panel for the eye and brain.

Learn about the importance of considering sex and gender in your research.

An updated look at how CRISPR is being used in therapeudic applications across a variety of diseases.