We narrowed to 30 results for: donor sgrna

...depending on perspective – to work with inefficient sgRNAs in my initial direct screening efforts, which were...CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cells. Nucleic Acids Research...Single-Stranded and Long, Partially Single-Stranded DNA Donors in Caenorhabditis elegans. Genetics 210:781–787...Modifications Improve Potency and Efficacy of DNA Donors for Precision Genome Editing. BioRxiv. https://...354480 Ghanta KS, Mello CC (2020) Melting dsDNA Donor Molecules Greatly Improves Precision Genome Editing...

...specific genomic edits by copying from a DNA donor template . The donor template contains the desired sequence...out integration of donor DNA. Both types of CASTs have been found to achieve donor DNA integration for...systems contain two components: a guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme...cell along with the gRNA(s) and Cas enzyme. The donor DNA contains the desired edit as well as additional...homology arms. Depending on the application, the donor DNA may be a single-stranded oligonucleotide, double-stranded...for large edits or insertions. When designing the donor template, incorporating mutations that prevent further...should alter the PAM site or target sequence in your donor DNA with a silent mutation that does not change ...

...fused to an sgRNA used to direct Cas9 to a specific genomic locus. When fused to the sgRNA and co-expressed...dCas9 – the dCas9, sgRNA/RNA cargo complex localizes to the sequence targeted by the sgRNA. This allows scientists...machinery that brings the sgRNA/cargo RNA to the appropriate DNA locus. The sgRNA/RNA fusions retained their...construct. The sgRNA targeting Gaussia luciferase can be easily replaced with an sgRNA targeting your ...specific recruitment of multiple copies of VP64 to the sgRNA-targeted gene, resulting in increased activation...SV40, SFFV) or inducible (CMV-TO) cDNA expression, sgRNA (U6) expression, shRNA/shRNA-miR30 constitutive ...and shRNAs with a reporter gene, or (4) multiple sgRNAs with Cas9 for genome engineering. The versatility...

...specific genomic edits by copying from a DNA donor template . The donor template contains the desired sequence...out integration of donor DNA. Both types of CASTs have been found to achieve donor DNA integration for...systems contain two components: a guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme...cell along with the gRNA(s) and Cas enzyme. The donor DNA contains the desired edit as well as additional...homology arms. Depending on the application, the donor DNA may be a single-stranded oligonucleotide, double-stranded...for large edits or insertions. When designing the donor template, incorporating mutations that prevent further...should alter the PAM site or target sequence in your donor DNA with a silent mutation that does not change ...

...This makes mCyRFP1 a powerful tool for use as a donor in FRET (fluorescence resonance energy transfer)... Yasuda groups use both mCyFP1 and EGFP as FRET donors in experiments simultaneously measuring the activities... a single, unique, and constitutively expressed sgRNA targeting the 5’ end of each essential gene. Koo...

...The library was cloned into the Liu lab’s own pAc-sgRNA-Cas9 insect expression backbone, which expresses...gene are supplied to the reaction from a prebuilt donor plasmid library, available from Addgene. Site-specific...available). Second, they co-transfected short-arm donor vectors containing mAID and either Neo or Hygro ...that constitutively express Cas9 with a library of sgRNA expression vectors (containing ten guides against...obstacle. With CRISPR-X, dCas9 is used to recruit an sgRNA with MS2 hairpin binding sites, which then recruit...

...Luciferase Type Promoter Description PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla...NanoBRET : Constructs using NanoLuc® as a BRET energy donor and HaloTag labeled with a fluorophore as the acceptor...

...crRNA, (3) three transposase proteins, and (4) the donor DNA. By using a multi-spacer CRISPR array, CRISPR-transposons...sequence which could be adjusted by changing the sgRNA length. The system could be further refined by the...

...labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow.” Nat Biotechnol. 34(5) (2016... be expressed in a single vector, pCRISPRainbow-DONOR1, with dCas9 supplied by a separate vector. ...

...Walker Jackson 78624 sgRNA(MS2)_Prnp_SAM1 PRNP U6 Dementia Walker Jackson 78625 sgRNA(MS2)_Prnp_SAM2 PRNP...Hyman 87200 pX335 HTT sgRNA-b HTT U6 Huntington's Mahmoud Pouladi 87201 pX335 HTT sgRNA-a HTT U6 Huntington's...His T5 Parkinson's Suzanne Pfeffer 208392 Lenti-sgRNA-Cre-GpNLuc-sgmATM ATM Ataxia telangiectasia Gaorav...Hemmo Meyer 85845 pDONOR-SNCAe2-WT SNCA CAG Parkinson's Jens Schwamborn 85847 pDONOR-SNCAe3-WT SNCA CAG...Schwamborn 85848 pDONOR-SNCAe3-A53T SNCA CAG Parkinson's Jens Schwamborn 86154 pDONOR-PARK6e5-I368N PINK1...TARDBP-Exon1-ko-sgRNA1-SpCas9 TARDBP U6 ALS Rajat Rohatgi 107858 pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9 TARDBP...133762 px459-TDP-43 sgRNA1 TARDBP U6 ALS Shawn Ferguson 133763 px459-TDP 43 sgRNA2 TARDBP U6 ALS Shawn...