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Showing: 21 - 40 of 63 results

  5. Colony Formation Titering Assay

    Type
    Protocol
    ... serial dilutions of the lentiviral vector pHAGE EF1α dCas9-KRAB . The following day, cells were treated...

  6. Hot Plasmids - August 2020

    Type
    Blog Post
    ...serotypes for the Cre-dependent Flpo recombinase pEF1a-DIO-FLPo-WPRE-hGHpA, which is now also available...

  10. Cre-lox system

    Type
    Collection
    ...into BAC SP6 Bacterial Mercader Huber 108543 pLV-EF1-Cre-PGK-Puro Cre EF-1 alpha Lentiviral Alcudia 108639... mutant TEF1 Yeast Piper 60932 pPL5606_TEF1*-Cre_ADE2 Cre expressed at low levels mutant TEF1 Yeast Piper...11920 pBS500 EF1alpha-GFPcre Cre-GFP fusion EF-1 alpha Mammalian Sauer 11923 pBS598 EF1alpha-EGFPcre Cre-EGFP...pAAV-Ef1a-mCherry-IRES-Cre Cre and mCherry coexpression EF-1 alpha AAV Deisseroth 55635 pAAV-EF1a-sCre...AAV Deisseroth 55636 pAAV-EF1a-Cre Cre EF-1 alpha AAV Deisseroth 55638 pAAV-EF1a-vCre VCre EF-1 alpha AAV...60930 pPL5071_TEF1*-Cre_URA3 Cre expressed at low levels mutant TEF2 Yeast Piper 60931 pPL5608_TEF1*-Cre_TRP1...similar plasmids pSicoR PGK puro , pSicoR-Ef1a-mCh-Puro , pSicoR-Ef1a-mCh and pSicoR-mCh-empty Lentiviral Jacks...

  11. Control AAV Preps

    Type
    Collection
    ...45185 AAV-EF1a-BbTagBY EF1a TagBFP and EYFP Cre dependent 9 Sanes 45186 AAV-EF1a-BbChT EF1a mCherry and...pAAV-Ef1a-Coff/Fon-BFP EF1a BFP Flp dependent 8 Deisseroth 137134 pAAV-Ef1a-Coff/Fon-mCherry EF1a mCherry...Wickersham 137130 pAAV-Ef1a-Con/Foff 2.0-BFP EF1a BFP Cre dependent 8 Deisseroth 137133 pAAV-Ef1a-Con/Foff 2.0-...-mCherry EF1a mCherry Cre dependent 8 Deisseroth 137137 pAAV-Ef1a-Con/Foff 2.0-oScarlet EF1a oScarlet ... pAAV-Ef1a-fDIO mCherry EF1a mCherry Flp dependent 1, 2, 5, 8, 9, rg* Deisseroth 55641 pAAV-Ef1a-fDIO ... EYFP EF1a EYFP Flp dependent 1, 2, 5, 8, 9, rg* Deisseroth 128434 pAAV-Ef1a-fDIO-tdTomato EF1a tdTomato...137129 pAAV-Ef1a-Con/Fon-BFP EF1a BFP Cre and Flp dependent 8 Deisseroth 137132 pAAV-Ef1a-Con/Fon-mCherry...

  12. Retrograde AAV viral preps

    Type
    Collection
    ...27056 pAAV-Ef1a-DIO EYFP EF1a EYFP, Cre-dependent Control Deisseroth 55641 pAAV-Ef1a-fDIO EYFP EF1a EYFP, ...114470 pAAV-Ef1a-mCherry EF1a mCherry Control Deisseroth 114471 pAAV-Ef1a-fDIO mCherry EF1a mCherry, Flp-dependent... 55636 pAAV-EF1a-Cre EF1a Cre expression Recombinases Deisseroth 55637 pAAV-EF1a-Flpo EF1a Flpo expression...176756 AAV-EF1a-jGCaMP8f-WPRE Ef1a GCaMP8f Calcium sensor Looger 176757 AAV-EF1a-jGCaMP8m-WPRE EF1a GCaMP8m...Deisseroth 55638 pAAV-EF1a-vCre EF1a VCre expression Recombinases Deisseroth 87306 AAV pEF1a-DIO-FLPo-WPRE-hGHpA...-hGHpA EF1a Cre-dependent Flpo recombinase Recombinases Zhang 121675 pAAV-EF1a-fDIO-Cre EF1a Flp-dependent...176758 AAV-EF1a-jGCaMP8s-WPRE EF1a GCaMP8s Calcium sensor Looger 179254 AAV-CAG-jGCaMP8f-WPRE EF1a GCaMP8f...

  13. Biosensor AAV Preps

    Type
    Collection
    ...pAAV-Ef1a-sRGECO Ef1a sRGECO none Constitutive 8 Deisseroth 137126 pAAV-Ef1a-Con/Fon-sRGECO Ef1a sRGECO...pAAV-Ef1a-Con/Fon-GCaMP6F EF1a GCaMP6f none Cre and Flp dependent 5, 8 Deisseroth 137123 pAAV-Ef1a-Con/...-GCaMP6F EF1a GCaMP6f none Cre dependent 8 Deisseroth 137124 pAAV-Ef1a-Coff/Fon-GCaMP6F EF1a GCaMP6f none... pAAV-EF1a-Con/Fon-GCaMP6M EF1a GCaMP6m none Cre and Flp dependent 8 Deisseroth 137120 pAAV-Ef1a-Con/Foff...-GCaMP6M EF1a GCaMP6m none Cre dependent 8 Deisseroth 137121 pAAV-Ef1a-Coff/Fon-GCaMP6M EF1a GCaMP6m none... AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato EF1a GCaMP6s dTomato Cre dependent 1 Ting 105714 pAAV-Ef1a-fDIO-...pAAV-Ef1a-fDIO-GCaMP6s EF1a GCaMP6s none Flp dependent 8 Larsen 105715 pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s EF1a GCaMP6s...

  14. Deisseroth INTRSECT Collection

    Type
    Collection
    ...pAAV-EF1a-Cre None 55632 pAAV-Ef1a-mCherry-IRES-Cre None 55637 pAAV-EF1a-Flpo None 55634 pAAV-EF1a-mCherry-IRES-Flpo...pAAV-EF1a-mCherry-IRES-Flpo None 55638 pAAV-EF1a-vCre None 55635 pAAV-EF1a-sCre None 55633 pAAV-EF1a-mCherry-IRES-Dre None ... 55641 pAAV-Ef1a-fDIO EYFP Flp 55640 pAAV-Ef1a-dDIO hChR2(H134R)-EYFP Dre 55639 pAAV-Ef1a-fDIO hChR2(H134R...pAAV-Ef1a-Coff/Fon-EYFP Flp AND NOT Cre 137129 pAAV-Ef1a-Con/Fon-BFP Cre AND Flp 137130 pAAV-Ef1a-Con/...137135 pAAV-Ef1a-oScarlet None 137136 pAAV-Ef1a-Con/Fon-oScarlet Cre AND Flp 137137 pAAV-Ef1a-Con/Foff 2.0...137125 pAAV-Ef1a-sRGECO None 137126 pAAV-Ef1a-Con/Fon-sRGECO Cre AND Flp 137127 pAAV-Ef1a-Con/Foff 2.0...H134R)-EYFP Flp 126080 pAAV-Ef1a-sCreDIO hChR2(H134R)-eYFP Scre 126081 pAAV-Ef1a-vCreDIO hChR2(H134R)-eYFP...

  15. Recombinases AAV Preps

    Type
    Collection
    ...121675 pAAV-EF1a-fDIO-Cre EF1a none 1, 5, 8, 9, rg* Engel , Nectow 69570 pAAV-EF1a-N-CretrcintG EF1a none 1...Serotype(s) PI 140135 pAAV-EF1a-iCreV EF1a none 1, PHPeB Zeng 140136 pAAV-EF1a-iDreV EF1a none 1, PHPeB Zeng ...55634 pAAV-EF1a-mCherry-IRES-Flpo EF1a mCherry (not a fusion tag) 1, rg* Deisseroth 55637 pAAV-EF1a-Flpo EF1a... EF1a none 1, 2, rg* Deisseroth 87306 AAV pEF1a-DIO-FLPo-WPRE-hGHpA EF1a none 1, 2, 5, 8, 9, rg* Zhang...55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a mCherry (not a fusion tag) 8, rg* Deisseroth 55636 pAAV-EF1a-Cre EF1a...Zhang 75469 pAAV-EF1a-Flp-DOG-NW EF1a none 1 Cepko 183412 pAAV-CAG-FlpO CAG none 1, rg* Janelia VCre AAV ...Promoter Fluorophore/Tag Serotype(s) PI 55638 pAAV-EF1a-vCre EF1a none 8, rg* Deisseroth Cre AAV ID Name Promoter...

  16. Optogenetics AAV Preps

    Type
    Collection
    ...26968 pAAV-Ef1a-DIO ChETA-EYFP EF1a ChETA EYFP Cre dependent 1, 5, 9 Deisseroth 35497 pAAV-Ef1a-DIO C1V1...floxed-eNpHR-EYFP-WPRE-pA EF1a eNpHR EYFP Cre dependent 9 Deisseroth 26966 AAV-Ef1a-DIO eNpHR 3.0-EYFP EF1a eNpHR 3.0 ... Deisseroth 20297 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA EF1a ChR2/H134R mCherry Cre ...rg* Deisseroth 20298 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA EF1a ChR2/H134R EYFP Cre dependent...mCherry Flp dependent 1 Cepko 55639 pAAV-Ef1a-fDIO hChR2(H134R)-EYFP EF1a ChR2/H134R EYFP Flp dependent rg* ...Constitutive 9 Deisseroth 35509 pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP EF1a ChR2(E123T/T159C) EYFP Cre dependent... PHPeB Dimidschstein 100061 pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry EF1a C1V1 (t/t) mCherry Cre dependent ...

  17. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...105547-AAV9 pENN.AAV.EF1a.eGFP.WPRE.rBG Control James M. Wilson AV-9-PV2453 45185-AAV9 AAV-EF1a-BbTagBY Control...AAV5 pAAV-Ef1a-DIO eNpHR 3.0-EYFP Optogenetics Karl Deisseroth AV-5-26968P 26968-AAV5 pAAV-Ef1a-DIO ChETA-EYFP... pAAV-Ef1a-DIO C1V1 (t/t)-TS-EYFP Optogenetics Karl Deisseroth AV-9-35498M 100061-AAV9 pAAV-Ef1a-DIO C1V1...AAV9 pAAV-Ef1a-DIO hChR2(E123A)-EYFP Optogenetics Karl Deisseroth AV-9-35509 35509-AAV9 pAAV-Ef1a-DIO hChR2...Biosensor James M. Wilson AV-1-27056 27056-AAV1 pAAV-Ef1a-DIO EYFP Control Karl Deisseroth AV-1-ALL854 51502...Control James M. Wilson AV-2-27056 27056-AAV2 pAAV-Ef1a-DIO EYFP Control Karl Deisseroth AV-2-ALL854 51502...Control James M. Wilson AV-5-27056 27056-AAV5 pAAV-Ef1a-DIO EYFP Control Karl Deisseroth AV-5-PV0101 105530...

  18. Brain Initiative Collection

    Type
    Collection
    ...under the mammalian promoter (EF1a) Francois St-Pierre 179460-AAV1 pAAV-EF1a-DIO-JEDI-2P-WPRE Double floxed...eukaryotic Ef1a promoter after Cre-dependent recombination. Ofer Yizhar 198516-AAV5 pAAV_EF1a-DIO-PdCO-...signaling cascades. Michael Bruchas 139505-AAV8 pAAV-Ef1a-DIO-PPO-Venus AAV vector for Cre-dependent expression...expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory...signaling cascades. Michael Bruchas 139505-AAV9 pAAV-Ef1a-DIO-PPO-Venus AAV vector for Cre-dependent expression...signaling cascades. Michael Bruchas 140135-AAV1 pAAV-EF1a-iCreV can be used to generate AAV virus that will...

  19. Caltech Systemic Capsids

    Type
    Collection
    ...140135 pAAV-EF1a-iCreV EF1a light-inducible recombinase iCreV Zeng 140136 pAAV-EF1a-iDreV EF1a light-inducible...pAAV.CMV.PI.EGFP.WPRE.bGH CMV EGFP Control Wilson 105547 pENN.AAV.EF1a.eGFP.WPRE.rBG EF1a EGFP Control Wilson 114469 pAAV-CaMKIIa-mCherry...CamKIIa mCherry Control Deisseroth 114470 pAAV-Ef1a-mCherry EF1a mCherry Control Deisseroth 117382 hSyn1-eYFP...ChR2 Deisseroth 20298 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA EF1a Activator, Cre-dependent...light-inducible recombinase iDreV Zeng 140137 pAAV-EF1a-iFlpV EF1a light-inducible recombinase iFlpV Zeng 164450...

  20. Luciferase Plasmid Collection

    Type
    Collection
    ...Marcel Bruchez 108542 pLenti-EF1a-Luciferase-IRES-Blast-WPRE Firefly EF1α Lentiviral expression of firefly...transfection Sidi Chen 118412 ssAAV-EF1a-FLuc-WPRE-HgHpA_bac_293 Firefly EF1α AAV expression of firefly luciferase...backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla EF1α AAV9-CRISPR knockout plasmid. Renilla luciferase...luciferase William Kaelin 21375 pHIV-Luciferase Firefly EF1α/IRES Lentiviral expression of firefly luciferase...Attila Karsi 105621 Lenti-luciferase-P2A-Neo Firefly EF1α Lentiviral expression of firefly luciferase Christopher... Sam Gambhir 104587 pHIV-iRFP720-E2A-Luc Firefly EF1α Lentiviral expression of firefly luciferase and ... Plagge 118017 pLX313-Firefly luciferase Firefly EF1α Lentiviral expression of firefly luciferase William...
Showing: 21 - 40 of 63 results