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We narrowed to 55 results for: fp

Showing: 21 - 40 of 55 results

  1. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ... to an FP; when supplied with a luciferase substrate, the luciferase transfers energy to the FP, resulting...limited by a few caveats. Since fluorescent proteins (FP) require external light activation, you can’t use...you use the excitation wavelength to specify which FP emission you’ll capture. Since the Nano-lantern system...

  2. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...studies rely on direct fusion of fluorescent proteins (FPs) to a yeast gene of interest. These fluorescently...recently developed a series of blue, green, and red FPs that are codon optimized specifically for expression... on their findings, the authors recommend optimal FP combinations for use in yeast imaging, categorized...

  3. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ...a synthetic intron between a fluorescent protein (FP) and a 3xFLAG tag (see construct schematic below.... homology arms for your gene of interest into the FP-SEC vector using Gibson assembly. This construct ...creating a loss-of-function allele as well as a promoter-FP fusion for the gene of interest. L1/L2 larvae from...

  4. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...proteins (FPs) are the gold standard for genetically-encoded FRET donors (Fig. 1). Scores of FP variants...experiments. However, remember that, if you make an FP fusion, you are inserting about 20-25 kDa of protein... due to this bulky insertion are inevitable. Most FP fusions for FRET studies are at the N- or C-terminus...function of certain proteins. Rather than shove a bulky FP into your protein, you can site-specifically label...

  5. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...degradation the Ward lab used fluorescent proteins (FPs) fused to the AID* (minimal 44 amino acids auxin ...protein interest would also result in depletion of the FP, which can be easily visualized.  The tool-kit consists...CRISPR techniques. This tool-kit includes an array of FPs as well as additional useful tags, including 3xFLAG...

  6. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...Collections Botman-Teusink Yeast FP Collection Botman-Teusink Yeast FP Collection You May Also Like... ...Materials Fluorescent Protein Plasmids & Resources FP Tagging FP Overexpression Resources This comprehensive ...fluorescent proteins (FPs) in a variety of plasmids allows for constitutive overexpression of FPs and tagging of...of genes of interest with FPs in yeast. Characterization of some of the FPs in this collection can be ...Publication FP Overexpression Plasmids for constitutive overexpression of codon-optimized FPs in yeast. ...be found in Botman et al., 2019 . FP Tagging Yeast tagging vectors to create fusions of proteins of interest...available with URA3, SpHIS5 and KANMX markers. The FP together with the protothropic cassette can be amplified...

  7. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...same time in live cells using fluorescent proteins (FPs) can be problematic because excitation light can ... biological phenomena. The technique of coupling FPs with Luciferases can overcome these problems but ...engineered Renilla luciferase, to produce a series of FP-RLuc8 fusions for multicolor imaging. In all of these...coelenterazine intiates the fluorescent signal and these FP-RLuc8 fusions were dubbed Nano-Lanterns. RLuc8 is...

  8. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...you! In almost any case you would use a traditional FP fusion, a SunTag fusion could be used as well. Pros...kDa vs 24 kDa for GFP alone. Although traditional FP fusions can also affect protein activity, half-life...

  9. Fluorescent Protein Guide

    Type
    Collection
    ...Luciferase Plasmids Blog: Which FP Should I Use? Blog: How to Choose a FP For Multi-Color Imaging Blog: ...Dark Side of Fluorescent Protein Fusions with mOX FPs Fluorescent Protein Kits Blog: When is a Monomer ...about the many applications of fluorescent proteins (FPs) and to find the plasmids that are available from...biological activity, like transcription. Michael Davidson FP Collection Browse the Michael Davidson comprehensive...

  10. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...pushes NLS-PCP-GFP off of the mRNA, leaving only a red FP bound to the reporter. Because it contains a nuclear...protein binding sites to tether many copies of the FP-coat protein fusions to their reporter mRNAs. Using...

  11. Bacterial Expression Systems

    Type
    Collection
    ...and reconstruct the full FP. Find plasmids containing FPs and split FPs that can be fused to your proteins...fluorescent protein (FP) tags so you can express your protein of interest fused to FPs and monitor their ...each part of a split FP. When the candidate proteins interact, the pieces of the FP can get together and...Protein Guide to find an extensive collection of FPs, including photoactivatable, photoconvertible, and... interact together by fusing each of them to two FPs whose emission and excitation spectra overlap. That...proteins of interest interact, they will bring both FPs together and you will be able to detect fluorescence...

  12. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...calcium reporters use a single fluorescent protein (FP), requiring only a simple measurement. However, single...biosensor. Ratiometric measurements with certain single FPs (such as GEX or blue/green CEPIA) or through FRET...

  13. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...RFP variants The toolbox of fluorescent proteins (FP) for cellular imaging is constantly expanding. Case...the growing list of popular plasmids in Addgene’s FP collection. Just last month, Robert Campbell’s lab... to learn about the influence of structure on an FP’s properties. pBAD-LSSmCherry1 is a long Stokes shift...various RFPs. For tips on how to choose the right FP for your next experiment, check out our recent guest...

  16. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Fluorescent Protein (FP) Guide and the Michael Davidson FP Collection NLS Nuclear localization...Transient expression vector for eGFP in plants Find FP tagging vectors in budding yeast pHT101-mCherry -...into host genome MSCV-IRES-GFP or pMSCV-IRES-mCherry FP - Mammalian retroviral gene expression with GFP or...

  19. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...inactive Cas9 (dCas9) fused to a fluorescent protein (FP) can help visualize specific genomic loci using fluorescent...cells. Design your gRNA sequence to direct the dCas9-FP fusion to a specific genomic sequence - potential...

  20. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...BH domain tdTomato John Cooper *Fusions to other FPs can be found by searching the complete Michael Davidson...ensconsin mCherry William Bement *Fusions to other FPs can be found by searching the complete Michael Davidson...Zonula Occludens-1 GFP Alan Fanning *Fusions to other FPs can be found by searching the complete Michael Davidson...endosomes Rab11a DsRed Richard Pagano *Fusions to other FPs can be found by searching the complete Michael Davidson...
Showing: 21 - 40 of 55 results