We narrowed to 50 results for: gfp cassette

...system plasmids for CRISPR-based knock-in of EGFP-2A-PuroR cassette to the C-terminus of endogenous proteins...vector. The PITCh donor plasmid with an EGFP-2A-PuroR cassette, flanked by microhomologous sequences and...deposited PITCh plasmids were tested by fusing EGFP-2A-PuroR cassette to a nucleolar protein, fibrillarin (FBL... general cloning plasmid, and a prebuilt Unc-32::GFP targeting vector. Jorgensen Lab SapTrap CRISPR/Cas...cells that uses PCR to generate both an expression cassette for the Cas9-programming sgRNA and HR donors for...provide PCR templates for amplification of the tag (eg GFP, Flag, YFP, etc) and selection markers. Two independent... expression vector allows for efficient marker cassette FLP-out. Plasmids can be found associated with...

...nature of GFP fluorescence to create an improved fluorescent aptamer system. Knowing that GFP’s  structure... Experiments Learn more about GFP Controlling Protein Activity with GFP Resources at Addgene.org Find...increase its fluorescence, essentially mimicking GFP.  Though the aptamer, called Spinach, required longer...RNA-based scaffolds to contain and stabilize Broccoli cassettes, thereby extending their use into mammalian cells...

...kanamycin cassettes and were carefully designed to allow for easy removal of the resistance cassette via Cre...the transcription factors from a polycistronic cassette. However, it was unknown whether variations in...in gene expression from these polycistronic cassettes could lead to comparable experimental results. Using...compared different variants of the polycistronic cassettes and discovered reprogramming discrepancies, which... Klf4 isoform (KLF4s or KLF4L). Polycistronic cassettes that contained KLF4s had overall diminished KLF4...global gene expression patterns. Polycistronic cassettes that contained the KLF4L isoform had more robust...-positive assay) as well as stabilization (Nanog-GFP reporter activation and silencing of factor-linked...

...translation-blocking STOP cassette immediately following the promoter. The STOP cassette includes a mutant YFP...-2.1 construct can express one of four colors (n-GFP, RFP, YFP or M-CFP.) The construct contains two tandem...eliminating two color possibilities. The remaining cassette can invert as long as Cre remains expressed; once... and sequence overlap (coral mOrange2, jellyfish EGFP, and sea anemone mKate2.) They then successfully... is retained in Brainbow-3.0, but with mOrange2, EGFP and mKate2 as the fluorophores; mOrange2 is the ...

... an exogenous donor cassette, the donor cassette replaces the target site cassette in a double-crossover... sites (often called a "lox-STOP-lox" or "LSL" cassette) placed upstream of a gene of interest will prevent...-layered genetic control. Recombinase-mediated cassette exchange (RMCE): When matching but mutually incompatible...DOG New Tricks: Controlling Protein Activity with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and...Rolf Zeller Use this dual recombinase-mediated cassette exchange (dRMCE) plasmid kit to re-engineer conditional...

...considerably faster, with expression levels comparable to GFP during early embryogenesis (Figure 3). mScarlet-I3...through the middle of each embryo are shown, comparing GFP, wrmScarlet (mScarlet), and the new mScarlet3 and...This strain is then transformed with the sgRNA cassette on a plasmid expressing mCherry and resistance... focal adhesion marker paxillin (visualized with GFP). Reprinted with permission from Zhong et al, 2024...shorter than many popular fluorescence markers like GFP (510 nm), allowing co-imaging. And because bioluminescent...

...reported was PA-GFP. PA-GFP was derived from Jellyfish (Aequorea victoria) GFP (wtGFP) by mutating Threonine...mutation in PA-GFP generates a chromophore population that is more neutral, causing PA-GFP to display almost...Markus, and Wei‐Lih Lee. "Photoactivatable GFP tagging cassettes for protein‐tracking studies in the budding...example, by engineering PA-GFP tagged to a mitochondrial matrix protein (mito-PAGFP) the Youle Lab was able...the neutral to the anionic form, which allows PA-GFP to exhibit up to a 100-fold increase in fluorescence...when illuminated with blue light (1). Similar to PA-GFP, scientists later developed red fluorescent PA-FPs...in vivo imaging models perfer the PA-RFPs over PA-GFP. Another class of irreversible PA-FPs are the photoconvertible...

...Bamburg 16407 pAdEasy 2-GFP beta-gal Test plasmid that contains β-gal and GFP and a ∼10 Kb genomic DNA...promoter Vogelstein 16404 pAdTrack For production of GFP-trackable viruses containing transgene under a chosen... Vogelstein 16405 pAdTrack-CMV For production of GFP-trackable viruses containing transgene under CMV ...pShuttle-CMV (Addgene #16403) plasmid with Gateway cassette Zhang 50958 ShuttleNSE For production of viruses...

...2) a transposon containing the gene-expression cassette that can translocate within the genome. The Sleeping..., hygromycin) and fluorescent protein reporters (GFP, BFP, RFP, no FP). The vectors each contain two SfiI...

... from the default GFP (green) to RFP, CFP, or YFP fluorescence. In Brainbow 2.1, GFP is tagged with a ...and activate fluorescence of the downstream OFP, GFP, or RFP. Cells that express the construct but do ... same XFP transgene in an adult fly. As a stop cassette is located directly upstream of the fluorescent...generates cells that randomly express either nuclear GFP, membrane CFP, cytoplasmic YFP, or cytoplasmic RFP...incorporates the highly photostable fluorophores mOrange2, EGFP, and mKate2 (red) to solve the problematically low...

...that incorporate a self-excising drug selection cassette (SEC). More information regarding this kit can...vectors for C. elegans research, including lacZ and GFP fusion vectors. C. elegans optimized fluorophores... promoter and expression of the target FLP-out cassette from either a ubiquitous or a tissue-specific ...

...containing an expression cassette with an ubiquitous promoter to express GFP in a group of young rats....label norepinephrine (NE) expressing neurons and GFP (green) to label neurons transduced by each serotype...

...protein (EGFP) reconstitution. (a) Scheme of validation for DSB mediated EGFP expression cassette reconstitution...annealing) took place and reconstituted the EGFP expression cassette. (b) pCAG-EGxxFP target plasmid and pX330...MCS) between EGFP fragments. The pX330 plasmid contains humanized Cas9 expression cassette and sgRNA expression...expression cassette. The target sgRNA can be cloned directionally into the BbsI site. (c) The pCAG-EGxxFP...halves of EGFP to recombine by homology directed repair, and resulting in the expression of EGFP. Using ...pCAG-EGxxFP target plasmid contains overlapping 5′ and 3′ EGFP fragments under the ubiquitous CAG promoter. The...indicates where your target is inserted, disrupting the EGFP fluorescent signal, for details on this plasmid,...

... their favorite selection cassette. They could also switch from FLAG to GFP or a different epitope. Addgene...

...individual expression cassettes, having the genes expressed from the same cassette is sometimes advantageous...and you can then insert the whole multicistronic cassette into a backbone as a single unit. Although it ...Multicistronic Element Expression Type MSCV-IRES-EGFP IRES Retroviral pMSCV-pBabeMCS-IRES-RFP IRES Retroviral...

...expression vector GFP Varies pMKO.1 GFP - Retroviral shRNA expression pLenti CMV GFP DEST - ...Fluorescent proteins (GFP, mCherry, etc) Localization pcDNA3-EGFP - C-terminal GFP for mammalian expression...genome MSCV-IRES-GFP or pMSCV-IRES-mCherry FP - Mammalian retroviral gene expression with GFP or mCherry selectable...fusing your protein to another protein, such as GFP, which allows you to visualize the cellular localization...create stable cell lines in which the expression cassette of interest is incorporated into the host... cells, integrate into host genome pLenti CMV GFP DEST - Lentiviral Gateway destination vector for ...PGKpuro2ABFP - For retroviral delivery of one sgRNA pMKO.1 GFP - Retroviral shRNA expression Retroviral backbones...

...markers were not increased compared to a control AAV-GFP. Not only did the AAV-SaCas9-sgRNA constructs mediate...genes in human cells using compact expression cassettes. Sci Rep 5: . https://doi.org/10.1038/srep10777...

...peptide polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles ... This makes it possible to use a polycistronic cassette that expresses multiple reprogramming factors ...

...vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) , DsRed (pSIR-DsRed-Express2) , human CD2 (...cloning All-in-one plasmid containing expression cassette for NmCas9 and BsmBI site for cloning in gRNA:...Musunuru CRISPRs in human pluripotent stem cells pCas9_GFP ; gRNA empty vector Protocol at StemBook O'Connor-Giles...plasmids: pVSVg , psPAX2 ; positive control: CMV-EGFP PDF, 2.4 MB Zhang GeCKO pooled library amplification...packaging plasmids: pVSVg , psPAX2 positive control: CMV-EGFP Pooled libraries are also available for human and...

...experiment, consider co-transfecting with GFP. This allows you to sort for GFP-positive cells and to enrich for...for example, plasmid with a puromycin resistance cassette, such as PX459). After you co-transfect the CRISPR...