We narrowed to 52 results for: golden gate

Guide to TALEN technology and using TALEN plasmids

Browse the Open Enzyme collection of plasmids at Addgene for generating enzymes for molecular biology lab work.

Find tips for transforming arabidopsis with your plasmid construct of interest.

An all-in-one CRISPR/Cas9 Multiplex Genome Engineering System for transfecting and editing cell lines.

Check out all of the plasmids featured in our hot plasmids articles from 2016.

Using plasmids for transformation, cloning, or transfection? Different uses mean you'll need different purities of DNA for your applications.

Gibson Assembly is a popular cloning alternative to traditional restriction/ligation methods. Learn more about this technique here.

The PUF Assembly Kit from Huimin Zhao's lab makes it possible to devise designer PUF proteins to hit any RNA target of interest.

An inside look at the ten most frequently distributed plasmid technologies at Addgene from the past ten years.

Find CRISPR plasmids to study the regulatory networks involved in retinal development.

Addgene's microbiology resources: Find plasmids for your microbe of interest, including bacteria, viruses, protozoa, fungi, and more.

This post describes applications of pooled libraries, briefly explains several approaches, and highlights the most popular pooled libraries at Addgene.

A list of free online molecular biology tools for plasmid mapping, DNA and protein sequence analysis, primer design and more.

REPLACR-mutagenesis is a new in vivo method for mutating your plasmid constructs. Learn how REPLACR stacks up against other mutagenesis techniques.

In this troubleshooting guide, find step-by-step tips and tricks for troubleshooting your cloning experiment.

Learn about new plasmids including optogenetic control of synaptic transmission, acoustic reporter genes, miRNA reporter vectors, and CRISPR-Cas9 delivery for in vivo screening.

We've partnered with SnapGene to update our plasmid maps. Learn how to use our new maps to accelerate your research.

Find bacterial expression plasmids that can be used for protein purification, protein visualization, study of protein interactions, controlled gene expression, reporter plasmids and genome engineering of bacteria.

Learn about different molecular cloning techniques, focusing on seven common cloning methods.

Sequence and ligation independent cloning (SLIC) lacks the strict requirements of many standard cloning techniques. Learn how to use SLIC in this post.