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We narrowed to 50 results for: golden gate

Showing: 21 - 40 of 50 results

  1. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...plasmid tools in about a week. Taking advantage of Golden Gate cloning, the Haynes lab’s Protocols.io protocol...histone-modifying enzymes and reader-effector proteins. b) Golden Gate assembly (backbones available at Addgene) with...

  2. Synthetic Biology - Overview

    Type
    Collection
    ...James Collins Tom Ellis Drew Endy Tobias Erb Susan Golden John Gray Jeff Hasty Dieter Jahn Alfonso Jaramillo...contains pre-assembled genetic circuits such as logic gates and higher level gene networks. Sensing and Signaling...Quorum Sensing Ellis Lab GeneGuard Endy Lab Logic Gates , BIOFAB Promoter/BCD Kit , and pOSIP Plasmid Kit...Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit GreenGate Cloning System (Plant) MoClo Modular Cloning Modular...Recombinant Phosphoprotein Kit S. cerevisiae Advanced Gateway Destination Vectors TAL Effectors Zinc Finger Consortium...

  3. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...are provided as empty backbones that utilize Golden Gate cloning to insert the desired guide RNA sequence...Brzezinski Lab CRISPR Collection The Brzezinski lab investigates gene regulation in the context of the developing...

  4. Tips for arabidopsis transformation

    Type
    Blog Post
    ... et al., 2007). Alternatively, if you prefer Golden Gate assembly, there are a series of plasmids from...and Dinneny, J. R. (2013) ‘A robust family of Golden Gate Agrobacterium vectors for plant synthetic biology...Plasmids from the Tsuyoshi Nakagawa lab are binary gateway vectors and can be used for promoter swapping and...transformant with a single insertion because it will segregate 3:1 in the T2 generation. You also have no control...genotype in T1 than T2. Your transgene should not segregate in T1, but it probably will in T2. Laura Lee...PMC4426443. 4. Nakagawa, T. et al. (2007) ‘Improved Gateway Binary Vectors: High-Performance Vectors for Creation...

  8. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ... destination vectors using low-cost, one-pot golden gate cloning that results in the creation of many ...Stephen Ekker’s lab, is a modified version of the Golden Gate TALEN kit (GGT) that streamlines the assembly...effector scaffolds previously constructed for the Golden Gate TALEN and TAL Effector Kit 2.0. Ma, et al. Hum...pCAG-T7-TALEN(Sangamo)-Destination series, pCAG-Golden-Gate-Esp3I-Destination Takashi Yamamoto pcDNA-TAL-NC2...Utah developed SapTrap. The SapTrap system uses golden gate assembly to produce single plasmid targeting ...relatively rare. The vectors are compatible with Golden Gate Cloning and Agrobacterium-mediated delivery making...pCAGGS-TAL-NC2 Charles Gersbach pcDNA3.1-GoldenGate, pcDNA3.1-GoldenGate-VP64 Maria-Elena Torres-Padilla pTALYM3...

  9. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    ... to the standard restriction/ligation, Gateway, or Golden Gate cloning methods. Whether it is for routine...actually work by significantly different methods. Golden Gate cloning does result in the seamless joining of...location and NOT in the middle of your insert. Gateway cloning utilizes λ integrase to catalyze directional...

  11. Cloning

    Type
    Guide
    ...Top Type IIS Assembly (Golden Gate & MoClo) Type IIS systems, such as Golden Gate and Modular Cloning (...to use MoClo and Golden Gate cloning kits for your next cloning project. Golden Gate Cloning (Image from...from Plasmid 101: Golden Gate Cloning ) Back to Top Ligation Independent Cloning Ligation Independent ...Back to Top Gateway® Recombination Cloning Gateway cloning (Image from Plasmid 101: Gateway Cloning ) Gateway... Gateway® cloning is a recombination based cloning method. The benefit of Gateway® is that moving a piece...cassettes). Although gateway cloning is a simple and efficient cloning method, gateway vectors and recombination...Guide Genome Engineering Guide Restriction Enzyme Gateway TOPO Gibson Type IIS Ligation Independent Cloning...

  13. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...restriction enzymes that are commonly used for Golden Gate cloning or for CRISPR gRNA cloning. With this...our SnapGene powered plasmid maps far easier to navigate. These practical and effective changes will give...

  14. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...Other Plasmid Cloning Techniques like SLIC and Golden Gate Cloning Learn How to Verify Your Plasmid Read...and the subsequent product is then digested and ligated into an appropriate vector. Gibson Assembly, GeneArt...maintained. Recombination efficiency is high enough to negate the need for selection usually required in recombineering...

  15. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    ..., the use of another cloning method, such as Golden Gate assembly, may also be beneficial. SLIC is most...through transformation, so the cloned vector must be ligated. But in LIC, longer single-stranded overhangs can...amounts of cloning. Assembly is scarless, unlike Gateway cloning, and the method’s flexibility allows it...

  17. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...consists of modular parts that are assembled with Golden Gate Assembly. They built this toolkit upon the Yeast...fluorescent proteins into one bright particle that segregates into one cell during cell division. To achieve...

  20. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...Strains Plasmids 101: TOPO Cloning Plasmids 101: Golden Gate Cloning Read all of our Plasmid Cloning Posts...recombinant plasmid to propagate. Any cells that contained non-recombinant vectors (re-ligated empty vectors, ...ccdB aspect, Gateway takes advantage of the same principle that cells will not propagate while expressing...recently developed Gibson Assembly Cloning and Gateway® cloning technologies, as well as several others...focus on a unique gene that makes the popular GatewayTM method possible: ccdB. But what is ccdB, what ...expressed the ccdB gene and were therefore unable to propagate because CcdA wasn’t available to counteract the... to thoroughly screen colonies for the insert. Gateway® technology (developed by InvitrogenTM) is essentially...
Showing: 21 - 40 of 50 results