...step rather than two. Stephen Ekker Golden Gate TALEN 2.0 Golden Gate cloning method. Validated in multiple...destination vectors for Voytas Golden Gate-assembly kit. 47875 pTALYM4 Golden Gate TALEN 2.0 48705 pTAL7a Boris...compatible with Golden Gate TALEN and TAL Effector Kit 2.0. Stephen Ekker Platinum Gate TALEN Kit Platinum...activities in cultured cells. Golden Gate TALEN 2.0 47388 pcDNA3.1-GoldenGate Charles Gersbach These new ...VP16) at the C terminus. 47389 pcDNA3.1-GoldenGate-VP64 Golden Gate TALEN 2.0 47874 pTALYM3 Maria-Elena Torres-Padilla...System Contains 336 pFusX clones allowing for Golden Gate assembly of 15-16 RVD TALEN inserts in one cloning...cells. Keith Joung Zhang Lab TALE Toolbox PCR/Golden Gate cloning method. Optimized for human expression...

...Simplified representation of the Voytas/Bogdanove Golden Gate TALEN kit. In this example, the final array is...that can be used with the recently developed Golden Gate cloning method to assemble “multiple DNA fragments...

...construct using Golden Gate assembly. Gersbach MuLE System Mammalian MultiSite Gateway recombination-based... of plasmids available for use in this Golden Gate and Gateway based assembly method: plasmids for assembly...nuclease/nickase expression cassette using the Golden Gate cloning method. A separate accessory pack is ... expression cassettes can be assembled using Golden Gate cloning or the Gibson Assembly. Chen Boosting...CRISPR/Cas9 vectors in one round of cloning by Golden Gate ligation or Gibson Assembly Liu A CRISPR/Cas9...Plasmids for assembly of gRNA cassettes using Golden Gate ligation into Tol2-based pGGDestTol2LC vector...a single T-DNA binary vector of choice through Gateway recombination. Qi Multiplex Conditional Mutagenesis...

...all Open Enzyme parts have C/E overhangs for Golden Gate assembly). Plasmids Explore the collection and...Endy Lab Plasmids Jennifer Molloy Lab Plasmids Golden Gate cloning...

...Bennink and Yewdell Labs Yarrowia lipolytica Golden Gate tool kit : Integrative vector assembly of up ...Toolkit - Borodina Lab Golden GATEway cloning system - Wittbrodt Lab MultiSite Gateway Kit : Creating three-part...oleaginous yeast Y. lipolytica - Rossignol Lab Yeast Gateway kit : 285 vectors for expression, markers, fusion...

...James Collins Tom Ellis Drew Endy Tobias Erb Susan Golden John Gray Jeff Hasty Dieter Jahn Alfonso Jaramillo...contains pre-assembled genetic circuits such as logic gates and higher level gene networks. Sensing and Signaling...Quorum Sensing Ellis Lab GeneGuard Endy Lab Logic Gates , BIOFAB Promoter/BCD Kit , and pOSIP Plasmid Kit...Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit GreenGate Cloning System (Plant) MoClo Modular Cloning Modular...Recombinant Phosphoprotein Kit S. cerevisiae Advanced Gateway Destination Vectors TAL Effectors Zinc Finger Consortium...

...are provided as empty backbones that utilize Golden Gate cloning to insert the desired guide RNA sequence...Brzezinski Lab CRISPR Collection The Brzezinski lab investigates gene regulation in the context of the developing...

...concentration of 1 μM). Ligate annealed oligos into pX330 using a Golden Gate assembly cloning strategy...