Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 44 results for: golden gate assembly

Showing: 21 - 40 of 44 results

  1. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    ...Data-optimized Assembly Design (DAD): This tool designs overhang sets for high complexity Golden Gate Reactions... for Goldengate cloning   Design primers for In-Fusion Cloning Design primers for Gibson Assembly and ... vector database: Find sequences for plasmids assembled from publications and commercially available sources...and NEB HiFi DNA Assembly  OligoAnalyzer Tool from IDT: For hairpin analysis, Tm and primer dimer estimation...methods, such as restriction enzyme cloning, Gibson assembly, or In-Fusion cloning.  NEB Ligation Calculator...

  2. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    ...such as Golden Gate assembly, may also be beneficial. SLIC is most often compared to Gibson assembly, a cloning...multicomponent assembly, as fragments with overlapping sequence homology can be assembled in a unique order...multicomponent assembly have 5′ or 3′ sequence homology to each other, they may be assembled incorrectly.... one option is to perform a hierarchical assembly, assembling fragments in multiple steps to avoid using...required for Gibson assembly. SLIC is a standardized method for multi-fragment DNA assembly, and its low cost... doing large amounts of cloning. Assembly is scarless, unlike Gateway cloning, and the method’s flexibility... cheap, standardized, and rapid multi-part DNA assembly method. Read on to learn how to use it in your...

  3. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...Other Plasmid Cloning Techniques like SLIC and Golden Gate Cloning Learn How to Verify Your Plasmid Read...product is then digested and ligated into an appropriate vector. Gibson Assembly, GeneArt Cloning, and SLIC...insertions require complicated, often costly in vitro assembly methods. A new method, REPLACR-mutagenesis, harnesses...substitutions - at the same efficiency as Gibson Assembly and GeneArt cloning - but at a much lower cost...mutagenesis methods Current SDM methods rely on in vitro assembly. To make a point mutation, you design two oligonucleotide...SLIC can all be used to assemble multiple fragments, but these methods can be expensive because they require...al. wondered if, instead of relying on in vitro assembly, they could make the E. coli do the work by using...

  5. Distribution to Industry

    Type
    Collection
    ...name Type PI Description High Complexity Golden Gate Assembly Standards Kit Cloning Eric Cantor Kit for... predict high accuracy junction sets for Golden Gate assembly. MoClo Toolkit Plant Expression Sylvestre...Sylvestre Marillonnet 95 plasmids for cloning and assembling standardized parts to create eukaryotic multigene...

  6. TALEN Guide

    Type
    Collection
    ...used with the recently developed Golden Gate cloning method to assemble “multiple DNA fragments in an ordered...Simplified representation of the Voytas/Bogdanove Golden Gate TALEN kit. In this example, the final array is...can be made. Intuitively, one would expect that assembling relatively small, repeat-laden DNA regions into...from the University of Minnesota, arrays can be assembled in a matter of days. In an open access Nucleic...custom arrays consisting of 12-31 repeats can be assembled and inserted into a variety of backbones in just... in August, uses a serial ligation protocol to assemble arrays. Dr. Joung, co-founder of the Zinc Finger...early in 2011 and they described their kit for assembling TAL effector arrays in a 2012 issue of Nature...

  7. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... for use in this Golden Gate and Gateway based assembly method: plasmids for assembly of single gRNA vectors...separate vectors and then assembled into one construct using Golden Gate assembly. Gersbach MuLE System Mammalian...expression cassettes can be assembled using Golden Gate cloning or the Gibson Assembly. Chen Boosting CRISPR...which can be assembled into the binary CRISPR/Cas9 vectors in one round of cloning by Golden Gate ligation...sgRNAs Zebrafish Plasmids for assembly of gRNA cassettes using Golden Gate ligation into Tol2-based pGGDestTol2LC...nuclease/nickase expression cassette using the Golden Gate cloning method. A separate accessory pack is ...components are then assembled into a single T-DNA binary vector of choice through Gateway recombination. Qi...

  8. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...Gibson Assembly, Golden Gate etc.), and try to find the simplest way to do it (i.e. avoid assembling too ...gel or by nanodrop to assemble the  fragments based on their molar ratio. Assembly of the fragments There...can generate the parts of DNA that you want to assemble together in a combination of different ways according...There are multiple ways you can assemble the different parts of a plasmid based on the cloning strategy...

  9. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...consists of modular parts that are assembled with Golden Gate Assembly. They built this toolkit upon the...relies on the assembly of fluorescent proteins into one bright particle that segregates into one cell ...microbiome systems. Find the toolkit for modular assembly of broad host range plasmids Counting cell division...fused the red fluorescent protein (RFP) to a self-assembling protein (SAP) such as a bacteriophage shell protein...microbiome bacteria with a toolkit for modular assembly of broad-host-range plasmids." ACS synthetic biology...

  10. MXS Chaining

    Type
    Blog Post
    ...Technology Pros Cons References Golden Gate Type II restriction enzymes Can assemble up to 10 modules in a single...2008 Gibson Cloning Gibson assembly method Single reaction, can assemble large DNA sequences, no restriction...same multiple cloning site (MCS). Modules are assembled one at a time by standard restriction enzyme digestion...upon ligation. As a result, once two modules are assembled next to each other, a third module cannot be placed...high-throughput cloning strategies available for assembling these modular types of constructs, and different...restriction enzyme-based). Well suited for the modular assembly of highly similar or repetitive sequences. Intermediate...called “cassettes”) can be readily reused in the assembly of any later construct (i.e.,functional cassettes...

  11. Microbiology Resources

    Type
    Collection
    ... Labs Yarrowia lipolytica Golden Gate tool kit : Integrative vector assembly of up to three transcription...Toolkit - Borodina Lab Golden GATEway cloning system - Wittbrodt Lab MultiSite Gateway Kit : Creating three-part...deposited tools and resources, or read our guide on assembly standards. Browse Plasmids by Microbe System Plasmids...chromosomes - Endy and Shearwin Labs Ralstonia-GG-Kit : Assembly of Ralstonia solanacearum species complex (Rssc...circuit design in E. coli - Freemont Lab MoClo-YTK : Assembly of single and multi-gene constructs for S. cerevisiae...perform your synbio experiment. Synthetic Biology Assembly Standards Guide Bacterial Expression Systems :...oleaginous yeast Y. lipolytica - Rossignol Lab Yeast Gateway kit : 285 vectors for expression, markers, fusion...

  12. Synthetic Biology - Overview

    Type
    Collection
    ...and more. Also contains pre-assembled genetic circuits such as logic gates and higher level gene networks... Cloning Kit GreenGate Cloning System (Plant) MoClo Modular Cloning Modular PUF Assembly Kit Optogenetics...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering SynBio...James Collins Tom Ellis Drew Endy Tobias Erb Susan Golden John Gray Jeff Hasty Dieter Jahn Alfonso Jaramillo...Quorum Sensing Ellis Lab GeneGuard Endy Lab Logic Gates , BIOFAB Promoter/BCD Kit , and pOSIP Plasmid Kit...Recombinant Phosphoprotein Kit S. cerevisiae Advanced Gateway Destination Vectors TAL Effectors Zinc Finger Consortium...

  13. Tips for arabidopsis transformation

    Type
    Blog Post
    ...al., 2007). Alternatively, if you prefer Golden Gate assembly, there are a series of plasmids from the...and Dinneny, J. R. (2013) ‘A robust family of Golden Gate Agrobacterium vectors for plant synthetic biology...Plasmids from the Tsuyoshi Nakagawa lab are binary gateway vectors and can be used for promoter swapping and...transformant with a single insertion because it will segregate 3:1 in the T2 generation. You also have no control...genotype in T1 than T2. Your transgene should not segregate in T1, but it probably will in T2. Laura Lee...PMC4426443. 4. Nakagawa, T. et al. (2007) ‘Improved Gateway Binary Vectors: High-Performance Vectors for Creation...

  15. Open Enzyme Collection

    Type
    Collection
    ...Open Enzyme parts have C/E overhangs for Golden Gate assembly. Plasmids Explore the collection and order...Endy Lab Plasmids Jennifer Molloy Lab Plasmids Golden Gate cloning...

  17. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...Strains Plasmids 101: TOPO Cloning Plasmids 101: Golden Gate Cloning Read all of our Plasmid Cloning Posts..., the more recently developed Gibson Assembly Cloning and Gateway® cloning technologies, as well as several...recombinant plasmid to propagate. Any cells that contained non-recombinant vectors (re-ligated empty vectors, ...ccdB aspect, Gateway takes advantage of the same principle that cells will not propagate while expressing...focus on a unique gene that makes the popular GatewayTM method possible: ccdB. But what is ccdB, what ...expressed the ccdB gene and were therefore unable to propagate because CcdA wasn’t available to counteract the... to thoroughly screen colonies for the insert. Gateway® technology (developed by InvitrogenTM) is essentially...

  18. Cloning

    Type
    Guide
    ...Gibson Assembly (Image from Plasmid 101: Gibson Assembly ) Back to Top Type IIS Assembly (Golden Gate & MoClo... next cloning project. Golden Gate Cloning (Image from Plasmid 101: Golden Gate Cloning ) Back to Top ...on TOPO cloning. Back to Top Gibson Assembly (Isothermal Assembly Reaction) Isothermal cloning, more commonly... MoClo) Type IIS systems, such as Golden Gate and Modular Cloning (MoClo) , take advantage of the unique... to assemble before you get started. Check out Addgene's website for easy to use MoClo and Golden Gate...Gibson assembly DNA fragments with 20-40 base pair homology at their ends can be easily ligated together... commonly known as Gibson assembly ( protocol ), takes advantage of the properties of 3 common molecular...

  19. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...to quickly assemble vectors and test different ideas. We are a huge fan of the Golden Gate based MoClo...expression method enables us to quickly screen and investigate transcription factor candidates and the regulation...

  20. Bacterial Expression Systems

    Type
    Collection
    ...promoters and purification tags compatible with Golden Gate modular cloning (MoClo) for use in E. coli . ...Toolkit (Addgene #1000000222) is a collection of Golden Gate compatible vectors for bimolecular fluorescence... in plants. The Zinc Finger Consortium Modular Assembly Kits (Addgene #1000000005, 1000000010) contain...Collection page to find plasmids and kits for efficient assembly of TALEN constructs. The Zinc Finger Consortium...
Showing: 21 - 40 of 44 results