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  1. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ...students. As part of this discussion, we mention graphs such as the one shown to the right that illustrate...but many students discuss the third year dip (see graph). Niroshi and Ben give their insights as to what...
  2. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...applications. Also, purifying GST by affinity chromatography requires proper folding of the tag, which prevents...to be purified by single step metal affinity chromatography. Purification of His fusion proteins from E...
  3. Lentiviral Guide

    Type
    Guide
    ...plasmids available from Addgene. 2nd Generation The graphic to the right shows how the lentiviral genome is...
  4. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...highly cited, we went to one of the largest bibliographic databases available, Web of Science. For each... publication, Web of Science provides basic bibliographic information and uses its vast network of articles...
  5. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...days of structural biology, Byron Rubin, a crystallographer working with Jane Richardson, provided an ...popularity was short-lived as powerful molecular graphics packages were soon developed that allowed researchers...
  6. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...analysis is complete, a new screen appears with a graphical representation of the results and a list of the... on the sample name or on its corresponding bar graph entry. Clicking to initiate the in-depth analysis...
  7. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ... decomposition analysis is performed yielding a graph of edits in the input population or clone.   ...sgRNA sequence. The TIDE software will generate a graph representing all insertions and deletions identified...
  8. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ... (SpyTag-GFP nanobody) assembled on beads. C) Micrograph of H1.8-GFP nucleosomes collected with MagIC-...of glutaraldehyde crosslinking: representative micrograph (C), particle orientation distribution and Fourier-shell...
  9. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...Like graphing data, choosing controls, or mixing clear solutions—public speaking is skill that any scientist...are easy to see (and for heavens sakes, label all graphs and data professionally). Do remember the fine ...
  10. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...universities are trying to compile more comprehensive demographic information about the postdoc work force, problems...sorted out there can be gaps in knowledge of demographics and the information is often confidential (see...
  11. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...directly manipulating affect an outcome (age, demographic, diet, etc., in a cancer drug clinical trial,...know type of distribution you are working with, graphing data with a histogram is sometimes sufficient ...
  12. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    .... I've found cooking, gardening, hiking, and photography to be very satisfying. Finally, many PFs are...distance runner, gardener, cook, and outdoor photographer.  Additional Resources on the Addgene Blog ...
Showing: 21 - 40 of 124 results