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We narrowed to 54 results for: lentiviral backbone

Showing: 21 - 40 of 54 results

  1. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...Stabilizing gRNA modifications  Sugar phosphate backbone modifications The recognition sequence for CRISPR-Cas9...modify the sugar or phosphate molecules of the RNA backbone to increase stability.  Nature has evolved ways...replaces a nonbridging phosphate oxygen. Other useful backbone modifications to consider include amide linkages...and a constrained ethyl.  Can’t decide which RNA backbone modification is best? Studies suggest coupling...Request ready-to-use viral preps of select CRISPR lentiviral plasmids Additional resources on the Addgene...

  2. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...compare their options for tools such as empty backbones, lentiviral packaging systems, and luciferase expression... Collections Lentiviral Plasmids Tetracyline Inducible Plasmids Empty Backbones Bacterial Expression... Top Five by Views Guides CRISPR Guide Lentiviral Guide AAV Guide Optogenetics Guide γ-Retrovirus...help users get started with tools like CRISPR, lentivirus, and optogenetics — and shared the protocols ...

  3. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    ...CombiGEM-based assembly into the CombiGEM lentiviral vector backbone pAWp12 (Figure 2). Cloning from the pAWp28...digestion and ligation steps to build barcoded lentiviral plasmids containing one or more gRNAs. As each..., the ligation products are a diverse pool of lentiviral plasmids. Multiple rounds of digestion and ligation... pAWp28 storage vector to the pAWp12 lentiviral vector should retain the diversity in your gRNA library...recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library." Nature biotechnology...

  4. Pooled Library Amplifications

    Type
    Blog Post
    ...to work with. For example, a library in a lentiviral backbone might be more prone to recombination, while...consider if there are any features of the library backbone that would make the library more difficult to ...while a library with a large backbone might be more difficult to transform.    Figure 1: Factors...other colonies containing smaller, recombined backbones that may replicate faster. For this reason, if...

  5. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    ...authorized to make and use lentivirus. Also, note that due to the size of the backbone, transformations require...conducted earlier by the Zhang lab for GeCKO, a lentiviral CRISPR library enabling genome-scale, knockout...that you review the steps for how to use pooled lentiviral CRISPR libraries and keep the following in mind... sequencing technology. These libraries are lentiviral in nature, so please ensure that you are equipped...

  6. Zhang Lab CRISPR Page

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    Collection
    ...2A Hygro resistance marker 61427 : lentiviral sgRNA cloning backbone with MS2 loops at tetraloop and stemloop...60231 : sgRNA cloning backbone with Cre recombinase-2A-EGFP-KASH Detailed backbone cloning information:...#61425 - #61427 are intended for lentivirus production : 61425 : lentiviral vector encoding dCAS9-VP64 with... top GeCKO Screening Library GeCKO are pooled lentiviral CRISPR libraries (both human and mouse) for performing...helper complex with 2A GFP 61424 : sgRNA cloning backbone with MS2 loops at tetraloop and stemloop - Contains...with 2A Blast resistance marker 61426 : lentiviral vector encoding the MS2-P65-HSF1 activator helper complex...luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone with luciferase-2A-Cre recombinase Vectors for...

  7. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...parentheses.  In this example I wanted to find a lentiviral plasmid to express ubiquitin under control of...300+ plasmids. Under ‘Vector Types’ I selected ‘Lentiviral’ which eliminates most of the plasmids designed.... S. cerevisiae) Plasmid Type: Choose an empty backbone if you intend to clone your own insert, or use...this filter will allow to refine by serotype or lentiviral prep Requests: Only see plasmids with more than... cell type Serotype: Choose the AAV capsid or lentiviral prep needed Tags: Identify a specific marker ...

  8. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...MSCV-IRES-Luciferase IRES Retroviral pWPI IRES Lentiviral AmCyan-P2A-mCherry P2A Mammalian pC5Kan-P2A...-P2A P2A Insect pUltra P2A and T2A Lentiviral Ac5-STABLE2-neo T2A Insect   For the co-expression...insert the whole multicistronic cassette into a backbone as a single unit. Although it is recommended to....) PubMed. 3. Highly Efficient Multicistronic Lentiviral Vectorswith Peptide 2A Sequences. Ibrahimi et...

  9. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...the authors packaged it into lentivirus and used the resulting lentiviral library to generate a population...biological applications. First, GMAP-compatible backbones for lentivirus LV 1-5 and retrovirus RV 2-5 were constructed...levels using next gen sequencing. The STARR-seq backbone is available from Addgene in both human and fly...Science. 2013. PubMed PMID: 23328393 Wu lab human lentiviral CRISPR library     CRISPR pooled libraries enable...expression validated GPCR sequences to any desired backbone.  Kroeze, et al. Nat Struct Mol Biol. 2015. PubMed...Liu lab’s own pAc-sgRNA-Cas9 insect expression backbone, which expresses the gRNA from a Drosophila U6...instance, the authors generated a GMAP-compatible backbone containing Rosa26 homology arms. The Rosa26 locus...

  10. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...comes to the backbone, shRNAs are most often expressed constitutively, either in lentiviral or retroviral...retroviral backbones. On some occasions, shRNAs are transiently expressed using either AAV backbones or species-specific...Designing the shRNA sequence Choosing the plasmid backbone Choosing the promoter Mature siRNAs typically...

  11. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...vectors for customized lentivirus production Finding a lentiviral vector backbone with just the right combination...to create a final lentiviral vector containing up to 5 different elements. Lentiviral vectors constructed... resistances, restriction enzymes and plasmid backbones. pUCXKT contains a nonfunctional truncated version...site in pUCXKT, removing the stop codons during backbone digestion and providing the missing codons for... both kanamycin and ampicillin (from the pUC19 backbone). *False positives can occur in this system if...collection of plasmids comprising the multiple lentiviral expression (MuLE) system from the laboratory ... combinations of genetic elements in a single lentiviral vector provides a powerful tool to modify mammalian...

  12. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...interest, painstakingly cloned into the perfect empty backbone, made sure to add the right tags to your gene,... NEB Stable   For cloning into and storage of lentiviral and retroviral vectors or cloning or repeated...JM109-derived. For cloning into and storage of lentiviral and retroviral vectors or cloning or repeated...Derived from HB101. For cloning into and storage of lentiviral and retroviral vectors or cloning or repeated...cells for cloning and storage of unstable DNA and lentiviral/retroviral vectors. Used by Addgene for pooled...

  13. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Fluorescent Proteins Empty Backbones Fluorescent Proteins: Empty Backbones Blue/UV Cyan Green Yellow Orange... present. Looking for more empty backbones? Visit our Empty Backbones page or search our complete collection...Explore empty plasmid backbones with different fluorescent tags to create fusion proteins with your gene...list many popular fluorescent proteins and empty backbones for expression and cloning of fusion proteins,...Prone to dimerization pLV-Azurite - Mammalian Lentiviral expression pB18cmBFPAzurite - Bacterial Expression...pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1-neo - Insect Expression... Mammalian Expression pLV-mCherry - Mammalian Lentiviral Expression pCS2+8CmCherry - Zebrafish/Xenopus...

  15. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...Encodes one insert, encodes gRNA/shRNA, or empty backbone. Purpose Answer “What does this plasmid ...: mammalian expression, bacterial expression, lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, ... (prone to recombination in bacteria) such as lentiviral, retroviral, and AAV plasmids, choose the NEB...

  16. A Tour of Addgene's Most Popular Pooled Libraries

    Type
    Blog Post
    ...is a collection of plasmids that share the same backbone and differ only in a small region. This makes ...In addition, several libraries are offered as lentiviral preparations to save you a few steps! To check...

  17. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ... optimal proportions from a vector backbone that allows lentiviral packaging as a single payload (Figure...tagging the kinesins KIF5A and KIF5B as well as backbones for generating other SNAPf-, CLIPf-, and CLAP-...

  19. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...Producing Lentiviral Particles E.1 Recommended materials E.2 Protocol for producing lentiviral particles...protocol for using the pLKO.1 cloning vector, a backbone used by the RNAi consortium for targeting human...optimal puromycin concentration F.3 Protocol for lentiviral infection and selection G. Safety H. References...RNAi Consortium The pLKO.1 cloning vector is the backbone upon which The RNAi Consortium has built a library...of pLKO.1 pLKO.1 is a replication-incompetent lentiviral vector chosen by the TRC for expression of shRNAs...direct transfection, or can be converted into lentiviral particles for subsequent infection of a target...analog). Due to the long terminal repeats found in lentiviral plasmids, Addgene currently recommends using ...

  20. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...expressed from different plasmid backbones. They noted that backbone differences such as the order, or...system was used to assess how different lentiviral plasmid backbone elements impact viral titer and to compare...expand the Fragmid toolkit to facilitate other lentivirus- and AAV-based studies. Unified MoClo Systems...
Showing: 21 - 40 of 54 results