We narrowed to 874 results for: lic

...- and ligation-independent cloning: A SLIC makeover In 2007, LIC received an important update, courtesy... and ligation-independent cloning (SLIC), eliminated many of LIC’s constraints by relying on the power...Ligation-independent cloning Ligation-independent cloning (LIC) was first developed in the 1990s. While traditional...transformation, so the cloned vector must be ligated. But in LIC, longer single-stranded overhangs can hold a nicked...repairable by the bacteria after transformation. LIC is a reliable cloning method, but it is limited by...back the entire 10–12 bases. As such, the use of LIC is often limited to specially designed plasmids. ...of DNA!) Figure 1: Schematic of SLIC. To start the SLIC cloning process, a gene of interest is ...

...species or metabolic pathways. A new study takes us one step closer to the vision of metabolic biofactories...s vision of ‘biofactories’, most successes in metabolic engineering have been limited to the overexpression...be host-independent, beyond the oft published metabolic engineering realm of S. cerevisiae and E. coli...control via scRNA design is readily apparent in metabolic engineering. The authors elegantly demonstrated...demonstrated the potential of this to control a metabolic network in S. cerevisiae by using three RNA programs ...programs, the authors sequentially rerouted the metabolic flux to generate differently pigmented derivatives...regulatory switch. By no means limited to the field of metabolic engineering, this is such a powerful and flexible...

...Sharing Policy Discussion PLOS’ New Data Policy: Public Access to Data PLOS’ New Data Policy: Part Two...supporting the research reported in PLOS publications. The policy was not an overnight revelation, rather...advice about the scope of the policy. End of story? Not quite. The PLOS policy on data access is a formal...Data Sharing Policies, etc.) and is founded on the principle that scientific publications must contain...clarified what the policy intent was and provided an FAQ list to further explain the policy. The fact that...This post was contributed by Jim Woodgett. Public Library of Science (PLOS) created a stir earlier this...year when it announced its data access and sharing policy. Since early March, the open access publisher has...

... : Kwabena's public health research, the HEAD outreach program, and educating the public about dietary...awarded plasmids from Addgene as a successful applicant to the Addgene, Seeding Labs Plasmid Grant. For...bring new opportunities to Ghanaian scientists Click here to listen! Podcast breakdown (Time : Topic)... public health...

...Initiative. Endy's team made news in March with a publication in Science describing the development of “transcriptors...It's wonderful to have Addgene as a professional public-benefit partner who shares our core values." Addgene...BIOFAB and BIL gates plasmid kits, the Biobricks Public Agreement, and the future of the bioeconomy. Addgene...work, and it begins with the central dogma - replication of DNA, transcription of DNA to make RNA, translation...

...research. This year, we put out another call for applications. We had an overwhelming response and were excited...sensor with proof-of-principle experiments that replicate current knowledge, but the sensor can also kinetically...  Michal Bernach: Developing an artificial leaf replica system to study plant-bacteria and bacteria-bacteria..., Bernach sought to develop an artificial leaf replica system. “This bottom-up approach will allow me ...Bernach explains. Developing an artificial leaf replica means creating a “leaf” with similar permeability...fluorescent proteins are made. As the bacterial replicate, fluorescent proteins will be diluted out and ...

.... Feng Zhang LIC TAL Effector Assembly Kit Assembly by ligation-independent cloning (LIC). Validated in...Golden Gate TALEN 2.0 48705 pTAL7a Boris Greber Application of TALEN technology in hPSCs pTAL7a and pTAL7b...

...Parkinson's Pablo Rivera-Fuentes 111723 pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q23 HTT Flag CMV, P10...Huntington's Cheryl Arrowsmith 111724 pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q73 HTT Flag CMV, P10...Huntington's Cheryl Arrowsmith 111725 pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q79 HTT Flag CMV, P10...Huntington's Cheryl Arrowsmith 111726 pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q23 HTT Flag CMV, P10...Huntington's Cheryl Arrowsmith 111727 pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q54 HTT Flag CMV, P10...Huntington's Cheryl Arrowsmith 111728 pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q73 HTT Flag CMV, P10...Huntington's Cheryl Arrowsmith 111729 pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q79 HTT Flag CMV, P10...

...- Zebrafish/Xenopus/Worm/Sea urchin pET mCitrine LIC cloning vector (u-mCitrine) - Bacterial Expression...mOrange 548 562 49 6.5 2.5 h Monomer pET mOrange LIC cloning vector (u-mOrange) - Bacterial Expression...mCherry 587 610 16 4.5 15 min Monomer pcDNA3 mCherry LIC cloning vector (6B) - Mammalian Expression pLV-mCherry... - Zebrafish/Xenopus/Worm/Sea urchin pET mCherry LIC cloning vector (u-mCherry) - Bacterial Expression...and emission maxima (Stokes shift), useful for applications like multicolor imaging with better spectral...

...Raetz 26098 pCW-LIC 3xPtac Lactose/IPTG Escherichia coli Cheryl Arrowsmith 26094 pET28a-LIC T7-lacO Lactose...Bacteria are commonly used to create, store, and replicate plasmids of all types, but beyond that, researchers...Plasmid Series contains plasmids suitable for replication in many gram-negative bacteria with different...BiFC studies. ID Plasmid Fluorescent Protein Application PI 14030 14031 pCyPet-His pYPet-His CyPet (donor...FixK2 Blue light (470 nm) Escherichia coli Andreas Moeglich 68940 pRMC2 Pxyl/TetO Anhydrotetracycline (aTc...pR_FixK2 Blue light (470 nm) Escherichia coli Andreas Moeglich 26092 pET15-MHL T7-lacO Lactose/IPTG Escherichia...Escherichia coli Rahul Sarpeshkar 111614 pCdrA-gfpC Cyclic di-GMP Fluorescence (GFPmut2) Pseudomonas aeruginosa...

...E. coli for expression and purification ( pET28a-LIC or other pET vectors are recommended). The selection...

... bacterial expression system such as the pET GFP LIC cloning vector and the pOKD plasmids. The recombinant...bacteria. Learn more about the different CRISPR applications in plants.     Transgenic yeast as source of...

...bacterial expression vectors are inducible (e.g. pBAD LIC cloning vector (8A)) and have an epitope tag (6xHis...types of yeast expression vectors. Consider your applications and experimental design. You can create a new...more traditional approach of an autonomously replicating vector that carries either a wild-type version...a variety of other types of vectors for most applications used to study gene function. From making transgenic...

... all publications arising from the use of this vector. A.2 Map of pLKO.1 pLKO.1 is a replication-incompetent...terminal repeat. f1 ori f1 bacterial origin of replication. Amp R Ampicillin resistance gene for selection...bacterial cells pUC ori pUC bacterial origin of replication. 5’LTR 5’ long terminal repeat. RRE Rev response...it has been diluted. Mix by swirling or gently flicking the tube. Incubate for 5 minutes at room temperature...the walls of the tube. Mix by swirling or gently flicking the tube. f. Incubate for 20-30 minutes at room... on the size of your target plate. TIP: MOI (multiplicity of infection) refers to the number of infecting...Appendix I.1. Sequence of pLKO.1 TRC-Cloning Vector Click here to see the sequence of pLKO.1 TRC-cloning vector...

...B): LiCl selectively precipitates RNA versus DNA or proteins. Add the correct amount of 7.5 M LiCl solution...solution to bring the concentration of LiCl in the extracted aqueous layer to 2.5 M. Incubate at -20°C ... not affect the quality of RNA or downstream applications. To improve yield of RNA, instead of incubating...

...Load 5 μL of each standard in duplicate Load 5 μL of each sample in duplicate. Do not forget to include a...dilution is appropriate). Quality of duplicates: Exclude duplicates from analyses if there is more than...Considerations Always run standards and samples in duplicate at least If possible, include an AAV reference...standard with DNase ** Make 6 serial dilutions, in duplicate, of your standard curve plasmid (2 x 10 9 stock...

...The amount of DNA that you cut depends on your application. A diagnostic digest typically involves ∼500 ...volume usually varies from 10-50 µL depending on application and is largely determined by the volume of DNA...manufacturer’s instructions. Pro-Tip Depending on the application and the amount of DNA in the reaction, incubation...you will be using the digested DNA for another application (such as a digestion with another enzyme in a...

...bacteria are used as the means for both storing and replicating plasmids. Because of this, nearly all plasmids...expression) carry both a bacterial origin of replication and an antibiotic resistance gene for use as ... microcentrifuge or falcon tube. GENTLY mix by flicking the bottom of the tube with your finger a few ...

...antibody can be purified for use in a variety of applications. Sharing speeds science. We believe that sharing...antibody-expressing plasmids containing the SV40 origin of replication use a HEK293 line stably expressing the SV40 ... containing the Epstein-Barr virus origin of replication use a HEK293 line stable expressing Epstein Barr...

...sufficient quantities of library for experimental applications. Repeated amplifications should be avoided as...required for bacterial propagation (origin of replication and antibiotic selection). This recombination...each 50 µL aliquot of thawed Endura Duos on ice. Flick gently to mix. Electroporate cells (one at a time...