...biosensor system was developed by constructing 14 luciferase donor Rluc8-Gα chimeras, four acceptor GFP2-Gγ...TRUPATH system, cells are transfected with plasmids expressing the GPCR of interest, a Gα-Rluc8 plasmid, and...

...type 2 (Th2) cells from mice that constitutively express Cas9 and screen for genes that affect Th2 differentiation...differentiation and activation, using expression of differentiation factors as the screen readout. This retroviral...ApoB-NanoLuc reporter by inserting the NanoLuc luciferase downstream of the apoB gene. Using this reporter...

... yeast expression, worm expression, insect expression, plant expression, mouse targeting, CRISPR, TALEN... mammalian expression, bacterial expression, lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, yeast...

...Description Expression Additional considerations CMV General expression mRNA Strong mammalian expression promoter...lower expression than U6. May have better expression in neuronal cells. U6 small RNA expression shRNA...some cell types. EF1a General expression mRNA Strong mammalian expression from human elongation factor ...give consistent expression regardless of cell type or physiology. SV40 General expression mRNA Mammalian...  Commonly used in expression systems for Drosophila. Polyhedrin General expression mRNA  Strong insect...Constitutive  Commonly used in expression systems for insect cells. CaMKIIa Gene expression for optogenetics mRNA...neuronal/CNS expression. Modulated by calcium and calmodulin. GAL1, 10 General expression mRNA Yeast ...

...the receptor, which can then activate expression of a luciferase reporter in the nucleus. The Roth lab...resource called PRESTO-TANGO (parallel receptorome expression and screening via transcriptional output - TANGO...preps from DREADD plasmids allow you to easily express a DREADD receptor in a specific neuronal subtype...

... for each gene that is expressed, multicistronic vectors simultaneously express two or more separate proteins...cells that are expressing your gene of interest at high levels. Unlike vectors that express screenable or...want to express multiple genes in the same cell. Although it is possible to drive co-expression by using... with multiple, individual expression cassettes, having the genes expressed from the same cassette is ...designed for bicistronic expression; however, many could be easily manipulated to express more than two genes...Co-expression of multiple genes is valuable in many experimental settings. To achieve this, scientists...multicistronic vectors? Detecting cells that are expressing your gene, especially if you are studying a novel...

...Knockdown the expression of Gene X using shRNA expressed from a plasmid   Figure 1: Expression Level of Gene...as the expression level of Gene X in cells treated with Plasmid A is similar to the expression level of...plasmid that constitutively expresses a reporter protein (e.g., GFP or luciferase) that is either co-transfected...decreased expression of Gene X. Ergo the positive control(s) should decrease the expression of Gene X....into the cells and the ability of the cells to express the protein.   In analysis of the result in Figure...Figure 1, an internal control, such as the GFP-expressing Plasmid B, could demonstrate whether the cells...cells were transfected successfully and expressing the protein. For example, fluorescence microscopy images...

... to eGFP expression Clevers 18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression; also expresses...expresses luciferase Lowe 9044 pMIG MSCV Plasmid for transgene expression; also expresses GFP. Also see...CMV/MSV lncRNA expression plasmid Sun 60683 pLXIN-Luc MoMSV Stable expression of luciferase in mammalian...Ecotropic MLV expressing envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG Envelope VSV-G-expressing envelope...GFP MoMLV U6-driven plasmid for shRNA expression; also expresses GFP. See pMKO.1 puro for Puro resistance...27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA; expresses rtTA. See Zuber et al. 2011 for additional... and infection results in long-term transgene expression. Some disadvantages of γ-retroviruses are that...

...accepts the sgRNA target sequence for U6 promoter expression and the second site accepts the homology arm ...-injection of the targeting vector and a Cas9 expression plasmid will insert the desired genetic tag and...donor plasmids for protein tagging, including luciferase (NanoLuc), fluorescent proteins (TagGFP2, TagBFP... then mixed and transfected along with a Cas9 expression vector. They can also be introduced directly ...directly into a Drosophila S2 cell line stably expressing Cas9. This PCR toolkit offers C- and N-terminal tagging... template for inducible alteration of protein expression levels. CRISPR tagging systems offer users an... tag endogenous proteins for studying protein expression, localization, and protein-protein interactions...

...same strain. MazF expression is under control of the AOX1 promoter whose expression is induced by methanol...that are used to drive high protein expression in vivo. Expression plasmids are often lost without plasmid... and James B. Kaper. "Use of stabilized luciferase-expressing plasmids to examine in vivo-induced promoters...hok/sok system to create strains that can stably express antigens in sufficient quantities to generate an...plasmid R1: Sok antisense RNA regulates hok gene expression indirectly through the overlapping mok gene."...

...cell-derived neurons expressing ASAP5-Kv. C) Cultured rat hippocampal neuron expressing pan-membrane ASAP5...Figure 2), the authors developed a cost-effective expression and labeling approach using these constructs ...protein of interest can be inserted. B) Protein expression and labeling procedure. Adapted from Smith et..., and CLAP-tagged proteins for mammalian cell expression. These plasmids will be useful not only for future...Janicot and Maziarz et al., 2024). Each plasmid expresses all the components of the biosensor in optimal... a YFP-tagged G protein and a NanoLuc® (Nluc) Luciferase-fused detector that specifically binds to active...

...transfection : 61422 : Expresses dCAS9-VP64 activator with 2A GFP 61423 : Expresses the MS2-P65-HSF1 activator...Cas9 (SpCas9), using one vector to express SpCas9, and another to express up to three sgRNAs (Swiech et al...to LacZ, plus luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone with luciferase-2A-Cre recombinase...AAV-KPL) This plasmid contains two expression cassettes, Renilla luciferase-2A-Cre recombinase and sgRNAs ...-ITR This plasmid contains two expression cassettes, Renilla luciferase-2A-Cre recombinase and an sgRNA...nuclei in addition to sgRNA expression. This plasmid contains two expression cassettes, Cre recombinase...

...a scientist to control signaling within cells expressing these engineered receptors. Chemogenetic receptors... the specific cell type where the receptor is expressed, these receptors can be used to artificially activate...luminescent opsins: fusion proteins of a light-emitting luciferase and a light-sensing optogenetic element, making... bimodal opto-chemogenetic actuator. When the luciferase enzyme oxidizes its substrate (luciferin), it...

...bacterial genome, and inserted it into E. coli to express and then test for any proteins that could cut RNA..., when they looked at the transformed E. coli expressing Cas7-11, they found no evidence of growth inhibition...RNA editing tools.   Fig. 1 Schematic of luciferase RNA knockdown assays using Cas7-11   Use ...

...binding sites increased activation of HIV promoter-luciferase constructs. In multiple HIV-1 latent T cell lines...HIV-1, measured via HIV-1 promoter-driven GFP expression. In cell lines that can produce HIV-1 toxic proteins... gRNAs to target the HIV-1 5’ and 3’ LTRs and expressed them along with Cas9 in the T-cell line 2D10. ...of the LTRs joined together. Clonal Cas9/gRNA-expressing cell populations were also immune to HIV-1 reinfection...cycle progression, or apoptosis with Cas9/gRNA expression. A pooled analysis found no evidence of Cas9 .... For two HIV-1 strains, Cas9/gRNA lentiviral expression significantly reduced the HIV-1 copy number, ...preventing re-infection would require sustained expression of Cas9/gRNAs, which could result in higher rates...

...backbones, lentiviral packaging systems, and luciferase expression. On individual material pages, we added ...added curation fields for purpose, promoters, expression types, and more, all of which help a researcher understand... Inducible Plasmids Empty Backbones Bacterial Expression Systems AAV Plasmids Blogs Origin of ...

... to Top Controlled Expression It isn’t always beneficial to constitutively express your gene of interest... on expression only at a specific time. This collection has a variety of plasmids whose expression levels...can be found in pCS-PesaRlux controlling luciferase expression. pCW-LIC 26098 3x Tac promoter (hybrid of...studying gene expression in Gram-negative bacteria. pAK Series Various Plasmids pTac GFP, Luciferase (LuxCDABE...Attila Karsi Broad host range vectors for expressing luciferase and fluorescent protein reporter genes in... bacterial expression systems to find out more! Genome Engineering Although overexpressing or knocking...plasmids used for expression in bacterial systems Plasmid...

... vitro, and can be used for measuring protein expression or localization in cells, or even single-particle....org Fluorescent Biosensors Collection Split Luciferase systems, including NanoLuc®, DULIP, LuTHy Other...

...solution is to restrict imaging analysis to cells expressing the lowest levels of the fusion protein. This...Protein Resources Find Other Plasmids that Glow (Luciferase Plasmids) Find Optogenetics Resources ...

...Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression Additional Resources: Browse...subsequent increase in expression by the activation domain, resulting in reduced gene expression. This idea of ... rtTA or TetR expression plasmid Stable cell lines can be made that continuously express a system component...Lentiviral, miRNA expression (PRIME) system for application in knockdown of gene expression at a single copy...copy in mammalian cells; Expresses firefly luciferase hairpin and GFP under pTREtight promoter None Either...promoters capable of controlling eukaryotic gene expression. Several endogenous promoters had been identified...coli presented the best candidates for inducible expression. Bacterial systems were tested for functionality...