We narrowed to 54 results for: luciferase expression

...biosensor system was developed by constructing 14 luciferase donor Rluc8-Gα chimeras, four acceptor GFP2-Gγ...TRUPATH system, cells are transfected with plasmids expressing the GPCR of interest, a Gα-Rluc8 plasmid, and...

...type 2 (Th2) cells from mice that constitutively express Cas9 and screen for genes that affect Th2 differentiation...differentiation and activation, using expression of differentiation factors as the screen readout. This retroviral...ApoB-NanoLuc reporter by inserting the NanoLuc luciferase downstream of the apoB gene. Using this reporter...

... yeast expression, worm expression, insect expression, plant expression, mouse targeting, CRISPR, TALEN... mammalian expression, bacterial expression, lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, yeast...

...Description Expression Additional considerations CMV General expression mRNA Strong mammalian expression promoter...lower expression than U6. May have better expression in neuronal cells. U6 small RNA expression shRNA...some cell types. EF1a General expression mRNA Strong mammalian expression from human elongation factor ...give consistent expression regardless of cell type or physiology. SV40 General expression mRNA Mammalian...  Commonly used in expression systems for Drosophila. Polyhedrin General expression mRNA  Strong insect...Constitutive  Commonly used in expression systems for insect cells. CaMKIIa Gene expression for optogenetics mRNA...neuronal/CNS expression. Modulated by calcium and calmodulin. GAL1, 10 General expression mRNA Yeast ...

...the receptor, which can then activate expression of a luciferase reporter in the nucleus. The Roth lab...resource called PRESTO-TANGO (parallel receptorome expression and screening via transcriptional output - TANGO...preps from DREADD plasmids allow you to easily express a DREADD receptor in a specific neuronal subtype...

... for each gene that is expressed, multicistronic vectors simultaneously express two or more separate proteins...cells that are expressing your gene of interest at high levels. Unlike vectors that express screenable or...want to express multiple genes in the same cell. Although it is possible to drive co-expression by using... with multiple, individual expression cassettes, having the genes expressed from the same cassette is ...designed for bicistronic expression; however, many could be easily manipulated to express more than two genes...Co-expression of multiple genes is valuable in many experimental settings. To achieve this, scientists...multicistronic vectors? Detecting cells that are expressing your gene, especially if you are studying a novel...

...Knockdown the expression of Gene X using shRNA expressed from a plasmid   Figure 1: Expression Level of Gene...as the expression level of Gene X in cells treated with Plasmid A is similar to the expression level of...plasmid that constitutively expresses a reporter protein (e.g., GFP or luciferase) that is either co-transfected...decreased expression of Gene X. Ergo the positive control(s) should decrease the expression of Gene X....into the cells and the ability of the cells to express the protein.   In analysis of the result in Figure...Figure 1, an internal control, such as the GFP-expressing Plasmid B, could demonstrate whether the cells...cells were transfected successfully and expressing the protein. For example, fluorescence microscopy images...

...accepts the sgRNA target sequence for U6 promoter expression and the second site accepts the homology arm ...-injection of the targeting vector and a Cas9 expression plasmid will insert the desired genetic tag and...donor plasmids for protein tagging, including luciferase (NanoLuc), fluorescent proteins (TagGFP2, TagBFP... then mixed and transfected along with a Cas9 expression vector. They can also be introduced directly ...directly into a Drosophila S2 cell line stably expressing Cas9. This PCR toolkit offers C- and N-terminal tagging... template for inducible alteration of protein expression levels. CRISPR tagging systems offer users an... tag endogenous proteins for studying protein expression, localization, and protein-protein interactions...

... mCherry Luciferase Lentiviral dual reporter vector expressing mCherry and firefly luciferase to simultaneously...105621 Lenti-luciferase-P2A-Neo Lentiviral reporter vector expressing firefly luciferase with neomycin... vector expressing firefly luciferase and ZsGreen. Alejandro Balazs 170674 pBOBI-FLuc Express firefly ...pCMV-FLuc Express firefly luciferase. Used in MLV-based SARS-CoV-2 pseudovirus assay. David Nemazee 161030...Zhou-pLOV-GFP-Luc Lentiviral vector expressing GFP and luciferase for use as a reporter in pseudovirus...can then be used to infect permissive cells that express ACE2, the cellular receptor for SARS-CoV-2, in ...Resources page for more information. Plasmids Expressing Spike and Spike Variants Full-length wildtype...

...NanoLuc luciferase-fusion protein expressed in cells. Additional Resources Browse Addgene's Luciferase Plasmid...Find luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing...The collection contains luciferase reporter vectors for sensitive gene expression analysis and protein fusion...HaloTag, SmBiT, LgBiT, HiBiT, and more. Luciferase Reporters Luciferase (Link opens in a new window) reporter...bright and stable luciferase that enables highly sensitive detection at endogenous expression levels. NanoLuc..., reporter, protein, or tag, such as "NFAT", "luciferase", "Ras", "NanoLuc", "HaloTag", "BiT", or other... from an enzymatic reaction to measure subtle expression changes with high sensitivity. These assays are...

...same strain. MazF expression is under control of the AOX1 promoter whose expression is induced by methanol...that are used to drive high protein expression in vivo. Expression plasmids are often lost without plasmid... and James B. Kaper. "Use of stabilized luciferase-expressing plasmids to examine in vivo-induced promoters...hok/sok system to create strains that can stably express antigens in sufficient quantities to generate an...plasmid R1: Sok antisense RNA regulates hok gene expression indirectly through the overlapping mok gene."...

...cell-derived neurons expressing ASAP5-Kv. C) Cultured rat hippocampal neuron expressing pan-membrane ASAP5...Figure 2), the authors developed a cost-effective expression and labeling approach using these constructs ...protein of interest can be inserted. B) Protein expression and labeling procedure. Adapted from Smith et..., and CLAP-tagged proteins for mammalian cell expression. These plasmids will be useful not only for future...Janicot and Maziarz et al., 2024). Each plasmid expresses all the components of the biosensor in optimal... a YFP-tagged G protein and a NanoLuc® (Nluc) Luciferase-fused detector that specifically binds to active...

...bacterial genome, and inserted it into E. coli to express and then test for any proteins that could cut RNA..., when they looked at the transformed E. coli expressing Cas7-11, they found no evidence of growth inhibition...RNA editing tools.   Fig. 1 Schematic of luciferase RNA knockdown assays using Cas7-11   Use ...

...binding sites increased activation of HIV promoter-luciferase constructs. In multiple HIV-1 latent T cell lines...HIV-1, measured via HIV-1 promoter-driven GFP expression. In cell lines that can produce HIV-1 toxic proteins... gRNAs to target the HIV-1 5’ and 3’ LTRs and expressed them along with Cas9 in the T-cell line 2D10. ...of the LTRs joined together. Clonal Cas9/gRNA-expressing cell populations were also immune to HIV-1 reinfection...cycle progression, or apoptosis with Cas9/gRNA expression. A pooled analysis found no evidence of Cas9 .... For two HIV-1 strains, Cas9/gRNA lentiviral expression significantly reduced the HIV-1 copy number, ...preventing re-infection would require sustained expression of Cas9/gRNAs, which could result in higher rates...

...backbones, lentiviral packaging systems, and luciferase expression. On individual material pages, we added ...added curation fields for purpose, promoters, expression types, and more, all of which help a researcher understand... Inducible Plasmids Empty Backbones Bacterial Expression Systems AAV Plasmids Blogs Origin of ...

...18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression with luciferase expression. Scott Lowe 9044...MSV lncRNA expression plasmid. Lei Sun 60683 pLXIN-Luc MoMSV Stable expression of luciferase in mammalian...GFP MoMLV U6-driven plasmid for shRNA expression with GFP expression. See plasmid 8452 for puromycin resistance...9044 pMIG MSCV Plasmid for transgene expression with GFP expression. See plasmid 12282 for the addition...27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA. Expresses rtTA. See Zuber et al. (2011) for additional...pMD2.G VSV-G-expressing envelope plasmid. Didier Trono 35617 pCAG-Eco Ecotropic MLV expressing envelope plasmid...Description PI 1780 pBABE-neo largeTcDNA MoMLV Expression of Large T antigen for creation of immortalized...

... to express a gene; however, we have included a function section for if you want to stably express an ...genetic element, or want to express shRNA. Choose by: Species-Specific Expression Epitope Tag or Fusion Protein...Selectable Markers Species-Specific Expression If you want to drive expression of your favorite gene, you will...EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian expression, Ligation Independent ...Empty mammalian expression plasmid for cloning untagged proteins pcDNA3.1-mCherry - Expresses mCherry tag ... Inducible Expression page Bacteria Lac, T7, araBAD, trp mTagBFP2-pBAD - Protein expression vector with...TEV cleavage Transient expression - Gradia Lab plasmids for bacterial expression, Ligation Independent ...

... vitro, and can be used for measuring protein expression or localization in cells, or even single-particle....org Fluorescent Biosensors Collection Split Luciferase systems, including NanoLuc®, DULIP, LuTHy Other...

...transfection : 61422 : Expresses dCAS9-VP64 activator with 2A GFP 61423 : Expresses the MS2-P65-HSF1 activator...Cas9 (SpCas9), using one vector to express SpCas9, and another to express up to three sgRNAs (Swiech et al...to LacZ, plus luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone with luciferase-2A-Cre recombinase...AAV-KPL) This plasmid contains two expression cassettes, Renilla luciferase-2A-Cre recombinase and sgRNAs ...-ITR This plasmid contains two expression cassettes, Renilla luciferase-2A-Cre recombinase and an sgRNA...nuclei in addition to sgRNA expression. This plasmid contains two expression cassettes, Cre recombinase...

...solution is to restrict imaging analysis to cells expressing the lowest levels of the fusion protein. This...Protein Resources Find Other Plasmids that Glow (Luciferase Plasmids) Find Optogenetics Resources ...