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We narrowed to 58 results for: mcherry reporter

Showing: 21 - 40 of 58 results

  1. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    ...remove a stop cassette upstream of an effector/reporter gene. In cells where the stop cassette is removed...https://doi.org/10.1101/788679 A destabilized GFP reporter for calcineurin activity in yeast Article contributed... it is an attractive drug target. Calcineurin reporters allow scientists to measure calcineurin dynamics... target calcineurin. Current calcineurin gene reporters are based on the bacterial βgal assay, and are...Büttner’s lab designed a calcineurin transcriptional reporter consisting of a quickly-degraded destabilized ...panels) equipped with destabilized GFP calcineurin reporter (blue) and stained with PI (pink) to indicate ...Image from Diessl et al., 2020. Find the GFP reporter calcineurin activity here Diessl et al., Microbial...

  3. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...for cloning untagged proteins pcDNA3.1-mCherry - Expresses mCherry tag in mammalian cells pBI-MCS-EGFP -...GFP, mCherry, etc) Localization pcDNA3-EGFP - C-terminal GFP for mammalian expression pLV-mCherry - 3rd...tagging vectors in budding yeast pHT101-mCherry - Expresses mCherry tagged fusion proteins in worms ins:MCS...MSCV-IRES-GFP or pMSCV-IRES-mCherry FP - Mammalian retroviral gene expression with GFP or mCherry selectable marker...luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase reporter plasmid...expression (Gateway) pYIC - Bicistronic fluorescent reporter gene with cap-dependent 3Myc-EYFP-HA-His6 and ...3rd gen lentiviral plasmid expressing mCherry pBad-EBFP2 - Bacterial expression of EBFP2 blue fluorescent...

  4. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ...a UAS-driven luciferase reporter construct. In the absence of GFP, no reporter output was observed in ... and a reporter construct into the mouse retina. GFP expression robustly activated the reporter gene TdTomato...and Cre-DOG, respectively, repurpose popular GFP reporter lines for more sophisticated experimental manipulations... commonly used red fluorescent proteins dsRed, mCherry, and TdTomato do not induce transcription. Thus...GFP. They then tested T-DDOGs with two mouse GFP reporter lines; again, TdTomato was seen only in cells ...tested in retinal electroporation studies, DsRed reporter expression was induced in ~76% of GFP+ cells, ...

  5. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...stabilization (Nanog-GFP reporter activation and silencing of factor-linked mCherry). They additionally discovered...are available at Addgene. Kim, et al. Stem Cell Reports. 2015. PubMed PMID: 25772473 Targeting and manipulating...enhancer elements and combined it with a variety of reporters (i.e. eGFP) and effectors (i.e. DREADDs). The ...last month, Robert Campbell’s lab added two new mCherry variants to the repository. These variants can ...ONE paper for more on the directed evolution of mCherry and the spectral properties of various RFPs. For...reagents shared via Addgene. Narayan S, et al. Cell Reports. 2017. PubMed PMID: 28813671 Listen to the podcast...Listen to Our Podcast Segment on PhoCl LSSmCherry1 & RDSmCherry1: Engineering and directed evolution of...

  6. Bacterial Expression Systems

    Type
    Collection
    ...Christopher Voigt 65008 pCRISPReporter-mCherry Promoter activity Fluorescence (mCherry) Escherichia coli Mattheos...Optogenetics for E. coli Return to top Reporter Plasmids Reporter plasmids can be used to detect events...proteins and specific DNA sequences. ID Plasmid Reports Reporter Mechanism Expression Species PI 14474 pRU1161...Tolker-Nielsen 90217 pMV762-Peredox-mCherry NADH:NAD+ ratio Fluorescence (mCherry) Mycobacterium sp. Ashwani Kumar...Fluorescence (mCherry) Escherichia coli David Summers 24659 pCHERRY3 Promoter activity Fluorescence (mCherry) Mycobacterium...protein interactions, controlled gene expression, reporter plasmids and genome engineering of bacteria. Plasmid...Visualization Protein Interactions Controlled Expression Reporter Plasmids Genome Engineering You may also like…...

  7. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...), tagRFP (pKa = 3.1) and mCherry (pKa = 3.8). We have observed that mCherry (and mTurquoise2, not shown...interest with an additional protein module that can report on location. This approach enables the study of...9759496.  3. Buckley, Anthony M., et al. "LOV-based reporters for fluorescence imaging." Current opinion in ...

  8. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...expression levels for these two reporters simultaneously. By fusing sfGFP and/or mCherry to genes of interest, ...phage attachment sites. The levels of the two reporters, mCherry and sfgfp in this case, can be controlled...or 20 matching nucleotides, the second binds to mcherry with either 0, 11, 18, or 20 matching nucleotides...cross‐talk. (A) Schematic of the strain expressing two reporters and dCas9 under a P-tet promoter integrated in...

  9. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type
    Blog Post
    ...followed by an internal ribosomal entry site and mCherry. If a variant has wild-type levels of abundance..., Bernach plans to use a suite of fluorescent reporters. Bacteria containing unstable fluorescent proteins...colonizer, labelled with a constitutively fluorescent reporter can also be used to track how one population responds...using TIMER and an unstable fluorescent protein reporter under the control of promoters that control cell... the TIMER growth rate bioreporter. During protein maturation, the bioreporter spontaneously changes from...fluorescence-activated cell sorting into four bins based on their eGFP:mCherry ratios. Genomic DNA from each bin are then extracted...artificial leaf, Bernach will use the CUSPER bioreporter such that fluorescent proteins expression is ...

  10. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ... PI 44965 NL4-3 mCherry Luciferase Lentiviral dual reporter vector expressing mCherry and firefly luciferase...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for...-CoV-2 spike plasmids, packaging plasmids, and reporter plasmids for viral pseudotyping with SARS-CoV-...Greene 105621 Lenti-luciferase-P2A-Neo Lentiviral reporter vector expressing firefly luciferase with neomycin... pHAGE-CMV-Luc2-IRES-ZsGreen-W Lentiviral dual reporter vector expressing firefly luciferase and ZsGreen...vector expressing GFP and luciferase for use as a reporter in pseudovirus production and infection. Dapeng...

  11. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...along with a reporter, such as a fluorescence gene or a resistance gene. These reporters allow you to ... using IRES elements. Further, scientists have reported lower expression of the downstream cistron due...eukaryotic cells, and, although some scientists report close to 100% cleavage with some of these peptides...that already has the multicistronic element and reporter cloned in. In these plasmids you would simply ... 2A element (depending on the placement of the reporter gene). Addgene's collection offers a variety of...Retroviral pWPI IRES Lentiviral AmCyan-P2A-mCherry P2A Mammalian pC5Kan-P2A P2A Insect pUltra ...multicistronic element(s), or you could replace one of the reporter genes above with your gene or genes of interest...

  12. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...second. The first successful irreversible PA-FP reported was PA-GFP. PA-GFP was derived from Jellyfish ...mutagenesis screening for enhanced RFP variants. PA-mCherry (E26V / A58T / K69N / L84F / N99K / S148L / I165V...UV-Violet or blue Green/ Red 4,500 High 553/ 573 PA-mCherries Monomer UV-Violet Dark/ Red >3,000 Medium 570/.... 2.Subach, Fedor V., et al. "Photoactivatable mCherry for high-resolution two-color fluorescence microscopy...

  13. Chemogenetics AAV Preps

    Type
    Collection
    ...and Activity Reporter/Tag Cre requirement Serotype PI 44361 pAAV-hSyn-DIO-hM3D(Gq)-mCherry hM3D(Gq) - Activation...pAAV-hSyn-DIO-hM4D(Gi)-mCherry hM4D(Gi) - Inhibition mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Bryan Roth...Roth 50458 pAAV-hSyn-DIO-rM3D(Gs)-mCherry rM3D(Gs) - Activation mCherry fusion Cre-dependent 8 Bryan Roth...Roth 50474 pAAV-hSyn-hM3D(Gq)-mCherry hM3D(Gq) - Activation mCherry fusion none 1, 2, 5, 8, 9, rg* Bryan...Bryan Roth 50475 pAAV-hSyn-hM4D(Gi)-mCherry hM4D(Gi) - Inhibition mCherry fusion none 1, 2, 5, 8, 9, rg* Bryan...154867 pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA hM4D(Gi) - Inhibition mCherry fusion Flp-dependent 8, rg* ...154868 pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA hM3D(Gq) - Activation mCherry fusion Flp-dependent 8, rg* ...

  14. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...p2A-ferritin-p2A-mCherry Mammalian Expression 74308 pcDNA3.0-Magneto2.0-p2A-mCherry Mammalian Expression...-ome using a quick, affordable, and ubiquitous reporter assay. In their recent publication, Kroeze et ...endogenous proteins. As described in their recent Cell Reports paper, the Kanemaki lab overcame this hurdle using...TRIBE is the low input required - McMahon et al., report using as few as 150 fly neurons as their starting...delivery of robust CRISPR activators We previously reported on a novel Cas9-based activator developed by the...and named it Magneto 2.0. Using Magneto2.0-p2A-mCherry constructs, the lab verified that Magneto2.0 was...Expression 74334 pCR8-Magneto2.0-p2A-mCherry Gateway 74333 pCR8-Magneto2.0 Gateway 74307 pAAV-CMV-DIO-Magneto2.0...

  15. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...calcium-binding sites as possible for added sensitivity. As reported in Nature Methods, the FRET changes of these Twitch...sensor for zinc using variants of mOrange2 and mCherry as donor and acceptor domains. He says, these new...

  16. MXS Chaining

    Type
    Blog Post
    ...produce complex fluorescent reporter constructs. These fluorescent reporters can be used to detect structure...protein sequences to develop diverse fluorescent reporters is a useful application of high-throughput cloning...the tyrosine-protein kinase Lyn) Membranes 3 mCherry 587nm/ 610 nm human β-Actin Actin 4 Citrine 516nm...

  17. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type
    Blog Post
    ...Fluorescent Protein (GFP/EGFP) for a transcriptional reporter or as a general cytoplasmic label of cells, there's...Incorporation into a FRET biosensor A FRET biosensor is a reporter that undergoes a conformational change to bring... which cannot form heterodimers (i.e. EGFP and mCherry). These simple strategies can help you make better...better fluorescent fusion protein reporters for your studies and greatly lower your chances of finding ...FP dimerization. Equally problematic, several reportedly monomeric FPs are not actually monomeric, at ...

  18. Hot Plasmids February 2024

    Type
    Blog Post
    ...Cas9-PAGE editing with viral sgRNA targeting mCherry reporter (mChe), with co-incubation with assist peptide.... CAP-Mac also robustly expressed fluorescent reporters in green monkeys and cultured human neurons, with...

  19. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ... with EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid...advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid family. They have made several ...

  20. Viral Production

    Type
    Collection
    ...in the absence of Cre. mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from...expression and/or function. These data are sometimes reported or posted on the material page for the corresponding...Pro cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at 1.7E6 viral genomes...recipient's initial thaw will be accounted for in our reported titers. Lentiviral vectors are titered using a...
Showing: 21 - 40 of 58 results