...DNA efficiently at the end of a linear piece (see NEB for more information). Thus, we recommend that you...

...England Biolabs (NEB) #R0552S EcoRI NEB #R0101S NEB buffer 1 NEB #B7001S NEB buffer 2 NEB #B7002S T4 DNA...DNA ligase NEB #M0202S T4 DNA ligase buffer NEB #B0202S DH5 alpha competent cells Invitrogen #18258-012...concentration, use 1 μL) 2 μL 10x NEB T4 DNA ligase buffer 1 μL NEB T4 DNA ligase add ddH 2 O to bring...catalog # DNA Miniprep Kit Qiagen #27104 EcoRI NEB #R0101S NcoI NEB #R0193S Agarose Sigma #A9539 D.2 Screening...enzyme manufacturer's resources, such as NEB’s online tool NEBcloner (Link opens in a new window) , to help...: 5 μL Forward oligo 5 μL Reverse oligo 5 μL 10x NEB buffer 2 35 μL ddH 2 O Incubate for 4 minutes at ...TRC-cloning vector (maxiprep or miniprep DNA) 5 μL 10x NEB buffer 1 1 μL AgeI add ddH 2 O to bring to 50 μL ...

... recombinant-deficient bacterial strains such as NEB Stable, Stbl3, JC8111, SURE2, or XL10-Gold will help...

...Resources Review of restriction modification systems NEB's Resource describing Dam and Dcm Methylases Discovery...

...restriction enzymes, visit (Link opens in a new window) NEB . Restriction enzyme digestion is commonly used in... in a new window) double digest finder tool from NEB . In a 1.5mL tube combine the following: DNA Restriction... sticky ends. See (Link opens in a new window) NEB's compatible cohesive ends chart . If you cannot find...restriction sites. See (Link opens in a new window) NEB's table of methylation sensitive restriction sites...concentration. Learn more at (Link opens in a new window) NEB's website about star activity . If you are digesting...

...ligation mix in different bacterial strains (stbl2, NEB stable, etc.), and/or use a different temperature...

...blog High Complexity Golden Gate Assembly with NEB’s DAD Interview with Nicola Patron on MoClo Plasmids...

...however, there are a few popular lab strains, such as NEB Stable, JM109, XL1 Blue or XL10 Gold, that are F’...

...reagents. Thermo Fisher Scientific Inc., Origene, and NEB® are all good places to look for some helpful kits...

...questions (besides Addgene, I know both Abcam and NEB have Ph.D.s answering emails and phones). We can’...

... plasmids with inverted terminal repeats. We use NEB Stable to transform and prep high quality DNA that...

...bacteria designed to minimize rearrangements such as NEB Stable or use a standard strain, like DH5α, grown...

...stability, such as some of our viral vectors, in NEB Stable cells. Resource information: This section ...

...expressed in SHuffle T7 Express competent E.coli (NEB) using IPTG induction. To biotinylate the nanobodies...

...viral production are propagated in the endA-mutated NEB Stable strain of E. coli. In addition, plasmids are...

...for storage and distribution at Addgene are DH5α, NEB stable, ccdb survival, and Pir1. The majority of ...

...time. Resources and References New England Biolabs (NEB) (Link opens in a new window) sells DNA Assembly ... also sell ET SSB (Link opens in a new window) . NEB has other resources, such as a primer design tool...Assembly kits and master mixes, including NEBuilder HiFi (Link opens in a new window) and Gibson Assembly (Link...

...to your own research needs. Other companies like NEB and ATCC can provide you with additional validated...

...al., 1981). E. coli strains such as DH5α, HB101, NEB Stable, Stbl3, etc., are recA- mutant strains, and...

...information about restriction enzymes check out NEB's website . Insert The insert is the gene, promoter...(lac- proAB) [F traDelta36 proAB lacIqZDeltaM15] NEB Stable New England Biolabs F' proA+B+ lacIq ∆(lacZ...