We narrowed to 130 results for: primer

Learn about antibody engineering, including what it is and why we like it. Deep dive into antibody directed evolution techniques, and learn about Addgene's partnership with the Institute for Protein Innovation (IPI).

Find a list of universal sequencing primers and other common sequencing primers.

High schoolers invented a new diagnostic for Lyme disease. In the process, they also created a tool for jointly designing RPA primers and Cas12a sgRNAs.

It's important to determine how much functional virus you have a in your prep before starting a transduction. Learn how to titer your lentivirus here.

FastCloning is a fast and easy technique that can replace restriction cloning. Learn about this great plasmid cloning technique here.

Find plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent proteins in yeast.

Learn how to use recombinase-based state machines from the Lu lab to monitor cellular events and regulate gene expression.

Learn how to use and apply NEBuilder HiFi DNA Assembly Technology from New England Biolabs for quick and accurate plasmid cloning.

This post provides tips for designing a prime editing experiment, from pegRNA design, PE tool selection, and choice of DNA sequence to edit.

Learn how we titer AAV for our viral service at Addgene and find tips for improving your AAV tittering methods.

Learn how to use in vitro screening and other tricks to expedite the CRISPR genome editing process in mice.

With the limitations of qRT-PCR based COVID-19 testing, scientists develop CRISPR-based diagnostic tools for COVID-19.

Find some tips and tricks for using CRISPR for gene editing.

Overview of Addgene's antibody production methods and quality control processes for purified recombinant antibodies.

Find prime editor plasmids for (search and replace) genome editing.

an overview of qPCR and ddPCR copy number variant assays.

Ever wondered how Addgene makes our viral preps? We explain it all here!

New to plasmids? Learn the basics of plasmids in this blog post and find out about the parts of a plasmid, how they are made, and how scientists can use them in the lab.

Learn how Addgene has worked to increase accessibility to our materials and educational resources.

Addgene is now using NGS services from seqWell Inc for plasmid validation. Learn how the seqWell sequencing process works here.