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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 85 results for: psin

Showing: 21 - 40 of 85 results

  1. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ... hSynap: Human Synapsin-1 promoter. Good for neuronal expression. hSynap-FLEX: Synapsin-1 promoter, FLEXed...expression in Cre-expressing cells. hSynap-FLP: Synapsin-1 promoter, FLP-dependent expression GFAP: Glial...

  3. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...to mCherry, all under the control of the human synapsin promoter. Purpose of this AAV pAAV-hSyn-DIO-hM4D...restricted to a particular cell type. The human synapsin promoter further specifies neuron-specific expression...PMC3069789. 3. Kügler S, Kilic E, Bähr M. Human synapsin 1 gene promoter confers highly neuron-specific...

  4. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...cations can affect the attachment of adherent cells) Trypsin EDTA (TrypLE, Thermo Fisher 12605-010) 6-well tissue... Isolate cells from the stable cell pool by trypsinization and break up any cell clumps into individual...expanded but before they become over-confluent, trypsinize the cells and expand them to larger culture dishes...the independent monoclonal lines. Pro-Tip When trypsinizing cells from the 96-well plate, use a microscope...detached from the plate and can be isolated. When trypsinized, detached cells will exhibit a rounded morphology...

  6. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ...encoding Cre recombinase under the control of the synapsin promoter. It can be used to drive Cre expression...AAV. Because Cre expression is controlled by the synapsin promoter, it and therefore mCherry will only be...

  7. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...Christopher Harvey’s lab. These plasmids localize opsins to the soma to avoid off target photostimulation... Plasmid Serotype Name 106174  AAVrg pAAV.hSynapsin.SF-iGluSnFR.A184S Viral vectors coming soon...

  9. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...controls: pAAV-hSyn-DIO-EGFP (50457-AAV5): The Synapsin promoter directs broad, neuronal expression. AAV...specific neurons. We recently packaged a soma-targeted opsin from Christopher Harvey’s lab. According to their...

  11. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...AAV-flex-taCasp3-TEVp here The red-shifted channelrhodopsin (C1V1) targeting parvalbumin cortical interneurons...use viral prep in serotype AAV5.  Get the channelrhodopsin here Astrocyte specific tight affinity glutamate...

  12. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...Activation of these neurons via the channel Channelrhodopsin 2 (ChR2) reduced glutamate transmission from...mouse to deliver Cre-dependent expression of  Channelrhodopsin 2 (ChR2), a modified receptor protein that...

  13. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...kinase II alpha (aCaMKII; excitatory neurons) and Synapsin-1 (pan-neuronal) promoters (Figure 1b), and sgRNAs... Neuron-specific expression is mediated by the synapsin 1 (SYN) promoter, which was superior in its ability...

  15. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ..., Adam Cohen's lab presented Archaerhodopsin 3 (Arch) as a new rhodopsin based voltage indicator for use...plasma membrane. To obtain a more sensitive channelrhodopsin actuator for triggering action potentials ...

  16. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...far-red fluorescent marker, a Flp-dependent channelrhodopsin in the retrograde serotype, and a dTomato ...

  17. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...optoGPCR" (coupled to Go, so it's an inhibitory opsin!) and due to its bistable nature, it enables inhibition...

  18. Newly Updated AAV Data Hub!

    Type
    Blog Post
    ...report describes a functional vector such as a channelrhodopsin Images showing the injection site as well ...
Showing: 21 - 40 of 85 results