...team describe the discovery of a light-sensitive opsin from algae that is sensitive to red light instead... channelrhodopsin... opsin...

... hSynap: Human Synapsin-1 promoter. Good for neuronal expression. hSynap-FLEX: Synapsin-1 promoter, FLEXed...expression in Cre-expressing cells. hSynap-FLP: Synapsin-1 promoter, FLP-dependent expression GFAP: Glial...

...to mCherry, all under the control of the human synapsin promoter. Purpose of this AAV pAAV-hSyn-DIO-hM4D...restricted to a particular cell type. The human synapsin promoter further specifies neuron-specific expression...PMC3069789. 3. Kügler S, Kilic E, Bähr M. Human synapsin 1 gene promoter confers highly neuron-specific...

...variations present in the data such as synapsin, hsyn synapsin-1, human synapsin, hSynap. Matching We capture ...

...cations can affect the attachment of adherent cells) Trypsin EDTA (TrypLE, Thermo Fisher 12605-010) 6-well tissue... Isolate cells from the stable cell pool by trypsinization and break up any cell clumps into individual...expanded but before they become over-confluent, trypsinize the cells and expand them to larger culture dishes...the independent monoclonal lines. Pro-Tip When trypsinizing cells from the 96-well plate, use a microscope...detached from the plate and can be isolated. When trypsinized, detached cells will exhibit a rounded morphology...

...2017. PubMed PMID: 28646204. soCoChR: A novel opsin that enables single-cell optogenetics Article contributed... the stray excitation of neighboring neurons in opsin dense regions. To overcome this issue, Shemesh et.... al. created a soma-targeted derivative of the opsin CoChR, termied soCoChR, by fusing a fragment of ...

...encoding Cre recombinase under the control of the synapsin promoter. It can be used to drive Cre expression...AAV. Because Cre expression is controlled by the synapsin promoter, it and therefore mCherry will only be...

...Christopher Harvey’s lab. These plasmids localize opsins to the soma to avoid off target photostimulation... Plasmid Serotype Name 106174  AAVrg pAAV.hSynapsin.SF-iGluSnFR.A184S Viral vectors coming soon...

...controls: pAAV-hSyn-DIO-EGFP (50457-AAV5): The Synapsin promoter directs broad, neuronal expression. AAV...specific neurons. We recently packaged a soma-targeted opsin from Christopher Harvey’s lab. According to their...

...Gottschalk lab has surveyed a range of microbial rhodopsin GEVIs in excitable cells of the nematode Caenorhabditis...membranes, which might beneficially influence the rhodopsin properties. Find these voltage biosensors at Addgene...

...receptor for SARS-CoV. The lab also found that cathepsins play a role in SARS-CoV infection and deposited...deposited plasmids for the expression of cathepsin S, L, and B. This became a great starting point for SARS-CoV...

...AAV-flex-taCasp3-TEVp here The red-shifted channelrhodopsin (C1V1) targeting parvalbumin cortical interneurons...use viral prep in serotype AAV5.  Get the channelrhodopsin here Astrocyte specific tight affinity glutamate...

...Activation of these neurons via the channel Channelrhodopsin 2 (ChR2) reduced glutamate transmission from...mouse to deliver Cre-dependent expression of  Channelrhodopsin 2 (ChR2), a modified receptor protein that...

...kinase II alpha (aCaMKII; excitatory neurons) and Synapsin-1 (pan-neuronal) promoters (Figure 1b), and sgRNAs... Neuron-specific expression is mediated by the synapsin 1 (SYN) promoter, which was superior in its ability...

...derived from direct comparative analysis of microbial opsins." Nature methods 9.2 (2012): 159-172.5. Goshen ...Addgene A Primer on Optogenetics: Introduction and Opsin Delivery The Materials Science of Optogenetics Experiments...

..., Adam Cohen's lab presented Archaerhodopsin 3 (Arch) as a new rhodopsin based voltage indicator for use...plasma membrane. To obtain a more sensitive channelrhodopsin actuator for triggering action potentials ...

...optoGPCR" (coupled to Go, so it's an inhibitory opsin!) and due to its bistable nature, it enables inhibition...

...far-red fluorescent marker, a Flp-dependent channelrhodopsin in the retrograde serotype, and a dTomato ...

...report describes a functional vector such as a channelrhodopsin Images showing the injection site as well ...

...injected retrograde AAVs at one location result in opsin expression in their cell bodies. Optical stimulation...