We narrowed to 67 results for: rpe

1. Antibodies 101: Epitope Tags

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   Blog Post

   ...tagging?   References and Resources References Terpe, K., Overview of tag protein fusions: from molecular...

2. The Strength of Story Telling

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   ...learning. Storytelling is a crucial part of interpersonal communication, which makes honing your ability...

3. The Crispr Babies Saga Shows the Need for Action, Not More Delays

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   ...Charlotte, all of whom are still working on theirpenmanship. Additional resources on the Addgene blog ...

4. Plasmids 101: Mammalian Vectors

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   ...are no "natural" mammalian ORIs, scientists have usurped viral-based ORIs to fill the void. These ORIs, ...

5. Flow Cytometry Readouts: Yes, No, and Everything in Between

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   ...She is currently a Research Assistant in Arlene Sharpe’s lab at Harvard Medical School.   More resources...

6. Antibodies 101: Flow Cytometry Controls

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   ...She is currently a research technician in Arlene Sharpe's lab at Harvard Medical School.   More resources...

7. CasPEDIA: A Functional Classification of Cas Enzymes

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   ...from the Innovative Genomics Institute      CRISPRpedia IGI’s digital resources in English/Spanish ...

8. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   ...immune cells has been a gap in the field. Arlene Sharpe’s group at the Harvard Medical School developed ...

9. 15 Years of Addgene: The Top 15 Plasmids

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   ...the envelope expressing plasmid pMD2.G. Find pMDLg/pRPE. scramble shRNA - This plasmid from David Sabatini...

10. Will You Be My Mentor? Finding and Asking for Mentoring Support

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    ...need for your current position Understand your interpersonal challenges if they know some of the people you...

11. CRISPR Guide

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    ...K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012). A programmable Dual-RNA–Guided DNA...Saunders, S. J., Barrangou, R., Brouns, S. J. J., Charpentier, E., Haft, D. H., Horvath, P., Moineau, S., Mojica...Biotechnology . 38 (7), 824–844. PMID: 32572269 Charpentier, M., Khedher, A. H. Y., Menoret, S., Brion, A...

12. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    ... Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E. A Programmable Dual-RNA–Guided DNA Endonuclease...

13. Viral Vectors 101: Chemogenetics

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    ....2016 Weston M, Kaserer T, Wu A, Mouravlev A, Carpenter JC, Snowball A, Knauss S, von Schimmelmann M, ...

14. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    ...validation experiments performed will be useful in perpetuity.  Second, plasmids are easy to store and share...

15. Cpf1 Update: Comparison to Cas9 and NgAgo

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    ...Shah SA, Saunders SJ, Barrangou R, Brouns SJ, Charpentier E, Haft DH, Horvath P, Moineau S, Mojica FJ, ...

16. Degrading DNA with Cascade-Cas3

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    ...Saunders, S. J., Barrangou, R., Brouns, S. J. J., Charpentier, E., Haft, D. H., Horvath, P., Moineau, S., Mojica...

17. Viral Vectors 101: Producing Your rAAV

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    ...depends on helper viruses like adenovirus (Ad) or herpes simplex virus to facilitate efficient replication...

18. Fluorescent Proteins 101: Fluorescent Protein Timers

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    ...experiments. In many cases fluorescent proteins have usurped old methods or complemented them in ways that make...

19. Antibodies 101: Producing Recombinant Antibodies

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    ...Mozziconacci O, Schöneich C, Freund E, Ricci MS, Carpenter JF, Lengsfeld CS. Do not drop: mechanical shock...

20. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    ... Duffraisse M, Furger E, Ajuria L, Giraud G, Vanderperre S, Paul R, Björklund M, Ahr D, Ahmed AW, Spinelli...