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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 611 results for: sam

Showing: 21 - 40 of 611 results

  1. Antibody Guide

    Type
    Collection
    ...application Collect and lyse samples to make proteins available. Tissue samples may need additional processing...culture samples to extract proteins. Homogenize (tissues only) and lyse. Cross-link the samples with formaldehyde...isotype. Overview of application Fix your samples. Prepare your samples using permeabilization, antigen retrieval...Blank controls in the form of sample buffer must also be run. Unique samples (non-biological replicates)...cultured B cells, where each culture should produce the same antibody clone, or from plasmid-based expression...factors, binding partners, location in the cell, and sample processing can hide or reveal epitopes on an antigen...contain antibodies targeting various epitopes on the same antigen. Polyclonal antibodies are often more environmentally...

  2. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...Run samples on 2% agarose gel at 10 V/cm using 1x Tris-acetate-EDTA (TAE) buffer. Examine samples for ...down to a smaller final volume if necessary. Run samples in a thermocycler using the following parameters...a mini-prep culture. Perform mini-prep for each sample and sequence each colony using a U6 promoter forward... the presence of intended genomic deletion. Run sample in thermocycler and run the following program: ...Numerous polymerases may be used for step 6.3 . Run samples in a thermocycler using the following parameters...to step 7.3 . Extract the gDNA from clones. Run sample in thermocycler: 65 °C for 6 min and 98 °C for ...min to extract gDNA. Screen each clone using the same PCR primers and reaction conditions optimized on...

  3. Chemogenetics AAV Preps

    Type
    Collection
    ...SYN PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition IRES EGFP none 5 Sternson 119744 AAV CAMKII PSAM4 GlyR...Flp-dependent 8, rg* Gether 119741 AAV SYN flex PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition IRES EGFP Cre-dependent...Gs) DREADD Inhibitory hM4D(Gi) DREADD KOR DREADD PSAM4 GlyR Promoter Synapsin CaMKIIa CD68 Dlx GFAP nEF...GlyR IRES EGFP PSAM4 GlyR - Inhibition IRES EGFP none 5 Sternson 121538 pAAV SYN1 HA-hM4D(Gi) hM4D(Gi) ...

  4. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...ERBB4 CMV ALS Yardena Samuels 29528 pcDNA3.1-ERBB4 (E317K) ERBB4 CMV ALS Yardena Samuels 29529 pcDNA3.1-ERBB4...ERBB4 CMV ALS Yardena Samuels 29530 pcDNA3.1-ERBB4 (E542K) ERBB4 CMV ALS Yardena Samuels 29531 pcDNA3.1-ERBB4...ERBB4 CMV ALS Yardena Samuels 29532 pcDNA3.1-ERBB4 (E563K) ERBB4 CMV ALS Yardena Samuels 29533 pcDNA3.1-ERBB4...ERBB4 CMV ALS Yardena Samuels 29534 pcDNA3.1-ERBB4 (E836K) ERBB4 CMV ALS Yardena Samuels 29535 pcDNA3.1-ERBB4...ERBB4 CMV ALS Yardena Samuels 29536 pCDF1-ERBB4 WT ERBB4 CMV ALS Yardena Samuels 29537 pCDF1-ERBB4 (E317K...ERBB4 CMV ALS Yardena Samuels 29538 pCDF1-ERBB4 (E452K) ERBB4 CMV ALS Yardena Samuels 29539 pCDF1-ERBB4 (...ERBB4 CMV ALS Yardena Samuels 29540 pCDF1-ERBB4 (R544W) ERBB4 CMV ALS Yardena Samuels 29541 pCDF1-ERBB4 (...

  5. Chemogenetics Plasmids

    Type
    Collection
    ...PSEMs). PSAMs are coupled to an ion pore domain to create a ligand-gated ion channel. Whether a PSAM is activating...and Pharmacologically Selective Actuator Modules (PSAMs). DREADDs are the most widely used chemogenetic ...activating, while hM4D(Gi) and KORD are inhibitory. PSAMs are engineered α7 nicotinic acetylcholine receptor...it is coupled to. Addgene has two main types of PSAMs: 5HT3 coupled, which are activating, and GlyR-coupled...

  6. Serotype Testing AAV

    Type
    Collection
    ...different serotypes. In addition to the standard 100 µL sample size, the vectors listed below are also available...production page . Note: For a list of all 100 µL sized sample, please see our Control AAV Inventory . The catalog...catalog ID for the 20 µL sized testing sample is denoted with a .T suffix (for example, AAV1.T). The catalog...catalog ID for the 100 µL sized sample does not have the suffix (for example, AAV1). AAV Vectors for Serotype...include virus associated DNA (vdna), which is a sample of the purified DNA from the original plasmid that...

  7. CRISPR Guide

    Type
    Collection
    ...protein, instead of a two-component system (Figure 9C) SAM activators - co-expression of dCas9-VP64 with a modified... ensures that all gRNAs will be expressed in the same cell. This is called multiplexing , and increases...deletions or inversions with two gRNA sites on the same chromosome. Most multiplex systems enable researchers...sequence ~1 kb shorter than SpCas9 while retaining the same basic function. Its size allows it to be efficiently...from Neisseria meningitidis ), which are about the same size as SaCas9, with higher fidelity than SpCas9... types of base edits: Base transitions - edit to same classification of nucleotide base (purine-to-purine...an enzyme evolved to excise inosine to access the same repair pathway and convert A to any other base. ...

  8. Antibody Production

    Type
    Collection
    ...total protein content of the sample is determined. Only antibody samples with >90% purity are distributed...using a NanoDrop spectrophotometer. If needed, the sample is adjusted by diluting or concentrating to a final...Coomassie staining. For Coomassie staining, the sample is separated via a denaturing SDS-PAGE gel alongside...

  9. Viral Production

    Type
    Collection
    ...of the viral capsid proteins VP1, VP2 and VP3. Samples of viral preparations are subjected to polyacrylamide...proteins as a fraction of total protein present in the sample is also determined and used to determine purity...shown relative to the total protein present in the sample. Sterility Viral vector preparations are added ...rigorous barcode matching system that follows the sample from the DNA tube all the way to the cleared viral...

  10. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...) If the target sites are on the same DNA strand (in cis) in the same direction, the sequence between ...orientation. A) If the target sites (loxP) are on the same DNA strand in opposite directions, recombination...different promoters. Expression of both fragments in the same cell reconstitutes a functional protein, allowing...

  11. AAV Viral Preps

    Type
    Collection
    ...Rabies Virus-Based Serotype Serotype Testing Samples Small samples of control AAV vectors for trial purposes...Service overview. Function Chemogenetics DREADDs, PSAMs Optogenetics Channelrhodopsins, Halorhodopsins, ...

  12. Brain Initiative Collection

    Type
    Collection
    ...eYFP Viviana Gradinaru 119741-AAV5 AAV SYN flex PSAM4 GlyR IRES EGFP Chemogenetics Cre-dependent expression...plasmid Scott Sternson 119741-AAV9 AAV SYN flex PSAM4 GlyR IRES EGFP Chemogenetics Cre-dependent expression...expression plasmid Scott Sternson 119742-AAV5 AAV SYN PSAM4 GlyR IRES EGFP Chemogenetics expression plasmid ...plasmid Scott Sternson 119744-AAV5 AAV CAMKII PSAM4 GlyR IRES EGFP Chemogenetics expression plasmid Scott Sternson...

  13. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...protein and a target-specific gRNA in the same cell at the same time. Single plasmids containing both the...75026 Worm R07E5.16 (U6) none S. pyogenes Boxem lentiSAMv2 75112 Mammalian/Lentiviral U6 yes, activate S...

  14. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...protein and a target-specific gRNA in the same cell at the same time. Single plasmids containing both the...

  15. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...protein and a target-specific gRNA in the same cell at the same time. Single plasmids containing both the...

  16. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...protein and a target-specific gRNA in the same cell at the same time. Single plasmids containing both the...

  17. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...protein and a target-specific gRNA in the same cell at the same time. Single plasmids containing both the...

  18. CRISPR Plasmids - Yeast

    Type
    Collection
    ...protein and a target-specific gRNA in the same cell at the same time. Single plasmids containing both the...

  19. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...protein and a target-specific gRNA in the same cell at the same time. Single plasmids containing both the...
Showing: 21 - 40 of 611 results