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We narrowed to 130 results for: tac promoter

Showing: 21 - 40 of 130 results

  2. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...400-500 bp cassettes containing the RNA pol III promoter, gRNA and pol III terminator. These relatively...together in a single vector. In addition, the pol III promoter is relatively weak, and low expression of gRNAs...biological systems. Size is also a factor; the pol lll promoter and terminator need be encoded only once, and ... loci. They also examined editing efficiency in intact rice plants, comparing traditional gRNA constructs...

  3. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ...different part types (e.g. 5’-GGAG and 3’-TACT flanking promoter parts, 5’-AATG and 3’-GCTT flanking CDS...MoClo kit, to construct a Level 1 vector with a promoter and terminator flanking the CDS, and a Level 2...start with are: Is your sequence of interest a promoter, a coding sequence, a terminator, or other plasmid...sequentially in order to properly ligate. PRO, promoter; CDS1, coding sequence with stop codon; CDSns,...

  4. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...new backbones special: Strong expression: A CMV promoter drives robust gene expression in mammalian cells...creation a breeze. Modular by design: Key elements (promoter, tag, polyA site) are flanked by unique restriction... both new and known regulators at the FOS gene promoter and the MYC locus (Figure 5). Figure 5...identification of heat-shock proteins at the FOS gene promoter using TurboCas, streptavidin immunoprecipitation...for two-photon (2P) imaging and loose-seal, cell-attached recordings in primary visual cortex (V1) layer...cells out of all BiPVe3-dTom cells. C) Confocal stack of a biocytin-filled cell expressing BiPVe3-dTomato...

  5. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...optimism they encapsulate, and the solutions they promote help motivate you to spend a little time advocating...fight is far from over here at home.  The work of promoting and defending the role of science in our democracies... take in abusing climate science skepticism to promote harmful policies that benefit polluting industries.In...marching in and of itself will be enough. IT WON’T! Promoting the incorporation of science in policy, demanding... that uses yeast cells to study how DNA damage promotes cancer. She is also a science communicator, UBC... programs, Canada has been here before.  These attacks on science ultimately galvanized scientists to ...

  6. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    ...and are easily adaptable for use in your lab! Promoting personal development through thoughtful planning...love to hear about the steps taken in your lab to promote personal development - let us know your strategies...with lab members, determine how to work through obstacles, and take steps to resolve interpersonal conflicts...

  7. Fluorescence Titering Assay

    Type
    Protocol
    .... Pro-Tip Different brands and lots of FBS can promote or inhibit transfection. Test a variety of brands...magnesium, Corning 21-040-CV (cations can affect the attachment of adherent cells) 0.45 μm polyethersulfone filter...

  8. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...majority of transfer plasmids use the weak viral LTR promoter to drive expression of the gene of interest. Consequently...have a chimeric 5'LTR that includes a CMV or RSV promoter as well as a portion of the 5'LTR. Including a...2nd-generation system because the wildtype 5'LTR promoter requires Tat to function. Third-generation plasmids...retroviruses. Lentiviruses are able to enter an intact nuclear membrane allowing them to infect both dividing...

  9. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...under-informed with respect to how different serotypes, promoters, and injection methods are best suited to their...containing an expression cassette with an ubiquitous promoter to express GFP in a group of young rats. We found...other important factors such as what is the best promoter to use, or if and how much the spread of the virus...performance) will be assigned citable DOIs through DataCite before being made publicly available through the...

  10. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...are therefore good temporal reporters to measure promoter activity, for example. Useful positive controls...dark after the transformation. Use introns Plant promoters can be recognized or leaky in Agrobacterium. This...of the GUS gene with the commonly used CaMV 35S promoter in Agrobacterium. This can be easily avoided by...engineered Agrobacterium strain is brought into contact with the leaf tissue and the T-DNA region with ...

  11. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...researchers test many different combinations of promoters, terminators, solubility and purification tags...2016). The variety of constitutive and inducible promoters, linkers, and tags makes EcoFlex a great option...been used to examine the effects of different promoters, terminators, enhancers, and cross-cassette interactions... et al., 2019). Many plasmid elements such as promoters or fluorophores are interchangeable across organisms...more! Engineering disease resistance through gene stacking, increasing production of a protein of interest....9b00322. Kim, Matthew S., et al. Degron-Based bioPROTACs for Controlling Signaling in CAR T Cells. Cold...

  12. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...search scoped to viral preps shows filters for promoter and serotype, among others. We also created a ...example, there is only one viral prep filter for promoter “Syn”, that, when selected, will match variations...technology that powers the new website search. Tackling findability from multiple angles    We assembled...

  13. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...light chain, each from a constitutively active CMV promoter. Constructs were then verified by both diagnostic... and heavy (blue) chains as driven by two CMV promoters (orange). Figure from Andrews, et. al., Elife  ...which in most cases left the functional light chain intact, the authors were able to greatly increase the ...

  14. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ... vectors within this kit contain a variety of promoters to facilitate gene expression in bacteria, yeast...plasmids within it contain a variety of different promoters followed by leader sequences (encoding small leader...different genes was predictable based solely on the promoters and Shine-Delgarno sequences used in this so-called...research. If you’d ever like to deposit a kit, please contact us at [email protected]. ...

  15. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...neuron-specific expression (hSyn promoter) and pan-mammalian cell expression (CAG promoter). An improved version ...indicators, these DNA constructs can be delivered to intact tissues and organs noninvasively and target a defined...

  16. Lentivirus Production

    Type
    Protocol
    .... Pro-Tip Different brands and lots of FBS can promote or inhibit transfection. Test a variety of brands...magnesium, Corning 21-040-CV (cations can affect the attachment of adherent cells) 0.45 μm polyethersulfone filter...

  17. Colony Formation Titering Assay

    Type
    Protocol
    .... Pro-Tip Different brands and lots of FBS can promote or inhibit transfection. Test a variety of brands...magnesium, Corning 21-040-CV (cations can affect the attachment of adherent cells) 0.1% Crystal Violet, Ward’...

  19. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...either the Thy1 or CAG promoter, while Brainbow AAV is under control of the EF1a promoter. Livet et al., ...known as SpyLigase and is a protein domain that promotes the formation of an isopeptide bond between 2 ...insert fragments of DNA containing basic parts (promoters, UTRs, coding sequences, terminators, etc) into... creating a single transcriptional unit (Ex: a promoter, 5’UTR, coding region, and terminator). Next, ...individual types of pre-cloned insert modules (plant promoter, N-terminal tag, coding sequence of the gene of...bringing the intracellular kinase domains into contact and initiating signaling. Grusch et al. demonstrate...

  20. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ... toxin gene (regulated by a rhamnose-inducible promoter) and a chromoprotein gene. Allelic exchange occurs...it is under the control of a rhamnose-inducible promoter. The resolution step leads to the desired mutation...mimicry and camouflage. An anemone’s red colored tentacles resemble worms which attract fish for them to ...
Showing: 21 - 40 of 130 results