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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 31 results for: tes

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Showing: 21 - 31 of 31 results

  2. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ... μL of TE. Top Protocol: Phenol-Chloroform Extraction of DNA Samples Add an equal volume of TE-saturated...Denaturing solution Renaturing solution 2 mg/mL RNase A TE or water-saturated phenol-chloroform Chloroform 100%...100% ethanol or isopropanol 90% ethanol 70% ethanol TE buffer 3 M Na-acetate (pH 4.8) Protocol: Generalized...the column using water or a neutral buffer such as TE. You will now have plasmid DNA that has been purified... Water-saturated phenol-chloroform can be used if TE-saturated is not available. Vortex microfuge tube... paper towel for 5-20 min. Resuspend dry DNA with TE (10 mM Tris-HCl pH 8, 0.1 mM EDTA). Pro-Tip DNA resuspension...

  7. Kit Free RNA Extraction

    Type
    Protocol
    ...Isoamyl alcohol (49:1) 75% Ethanol RNase-free water or TE solution RNase free tubes: microcentrifuge tubes,...residual ethanol wash and add RNase-free water or TE as soon as the entire tube is dried but while the...visible. Resuspend RNA pellet in RNase-free water or TE. Quantify and assess the quality of your RNA sample...

  9. Deisseroth INTRSECT Collection

    Type
    Collection
    ...GJ, Ramakrishnan C, Kozicz T, Deisseroth K, Thiele TE, McElligott ZA, Holmes A, Heisler LK, Kash TL. 2016...

  10. DNA Quantification

    Type
    Protocol
    ...readings than a DNA sample dissolved in buffer (such as TE). You will get much more accurate and consistent ...

  11. Ligation Independent Cloning

    Type
    Protocol
    ...digestion and PCR products with sterile water (instead of TE buffer) to ensure optimal salt concentrations in ...
Showing: 21 - 31 of 31 results