We narrowed to 27 results for: tet off

...some challenges still exist. Nucleases may cause off-target effects on regions of the genome with similar... the cell can occur. Similar systems include tetracycline or doxycycline mediated activation of Cre to...

...) the consequences to your experiment if there’s off-target expression.   Once you know that, you’re ready...tyrosine-hydroxylase (TH), can limit which cells express your gene. Off-target expression can still occur (Fig. 3) - for ... two astrocyte selective promoters demonstrating off-target expression in both oligodendrocytes (Olig2...promoter allows you to decide when to turn on, or turn off, gene expression by administering an inducing agent...  Finally, the risks of potential toxicity and/or off-target effects (e.g. recombinase-dependent vectors...2002). Lack of an Immune Response against the Tetracycline-Dependent Transactivator Correlates with Long-Term...

...has used an inducible MitoTimer reporter using a Tet-on system (pTRE-Tight-MitoTimer) to show its usefulness...peptide-peptide ligation using SpyLigase Building off their SpyTag/SpyCatcher system for protein-peptide...mutagenesis (K176R) to improve the otherwise slow off-kinetics (15.8 ± 0.4ms from 21.4 ± 1.1 ms); this ...duration. Both of these strategies carry the risk of off-target effects. Harald Janovjak and his team at the...RUSH) use Cre-mediated recombination to turn on or off the expression of shRNA. This system requires less...expression. The capability to turn RUSH and CRUSH alleles off or on enables this method to rapidly address questions...repressor plasmids from Scot Wolfe's lab. These include Tet-inducible CRISPR activators and repressor plasmids...

... underwent affinity screening to select for slow off-rate binders. The best candidates were validated ...where gRNA expression is induced by anhydrous tetracycline. Three new anti-CRISPRs were identified that...

...functional knock-out via frameshift and minimize off-target effects. The library was cloned into the Liu...unique tissue-specific promoters expressing GFP, tetracycline response elements, and shRNAs many of which ...inserting OsTIR1 into the "safe harbor" AAVS1 locus (a tet-inducible OsTIR1 plasmid is also available). Second...

... temporally control which genes are turned on or off in a given area and the can do so in a very specific...gene expression studies, which are evinced by the Tet system and visualization of single molecules via ... which allow you to easily and reversibly switch off and on your favourite signalling pathways (Figure...of the ribosome during translation, which knocks off all mRNA binding proteins as it travels along the...for Translation RNA Imaging by Coat protein Knock-off, is made up of three components: a GFP protein that...

...188572 Tet-off TauRD (P301L/V337M) MAPT Myc TRE Parkinson's, FTD Franz-Ulrich Hartl 188573 Tet-off Full ...