...Mombaerts 17408 Puro.Cre empty vector Cre, Puro resistance and miRNA expression PGK Lentiviral Jacks 17736...Iwasato 85577 pTC-CMV-Tet CreER expression and tetracyclin-dependent transgene/shRNA expression ApoE.HCR.hAAT...Ehmer 85578 pTC-ApoE-Tet CreER expression and tetracyclin-dependent transgene/shRNA expression ApoE.HCR.hAAT...

...populations, an antibiotic resistance gene (i.e., a gene whose product confers resistance to ampicillin) is included...providing a benefit to the host, such as antibiotic resistance. This benefit can be context-dependent, and thus...includes a DNA replication origin, an antibiotic-resistance gene, and a region in which exogenous DNA fragments... origin of replication ( ori ), an antibiotic-resistance gene, and at least one unique restriction enzyme...plasmids are convenient and easy to use. Antibiotic Resistance Gene Allows for selection of plasmid-containing... important to distinguish that the antibiotic resistance gene is under the control of a bacterial promoter...is typically in the form of another antibiotic resistance gene (this time, under the control of a non-bacterial...

...including but not limited to cell growth and drug resistance. Recently, the Wu Lab at Texas Tech developed...vectors containing mAID and either Neo or Hygro resistance markers with CRISPR/Cas vectors targeting their...efficient approach to identify genes involved in drug-resistance. Remarkably, this method also enabled Lourido...proteasome subunit PSMB5 to find mechanisms of resistance to a proteasome inhibitor; CRISPR-X identified...unique tissue-specific promoters expressing GFP, tetracycline response elements, and shRNAs many of which ...