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We narrowed to 130 results for: yeast expression

Showing: 21 - 40 of 130 results

  1. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...conveniently allow for unlimited production, reliable expression, and easy distribution as DNA (Trimmer, 2020)...antibody production can take place in bacterial, yeast, plant, or mammalian cells. Each of these systems...advantages and disadvantages. Briefly, bacterial and yeast cells are cost-effective and faster-growing than...post-translational modifications that bacterial and most yeast cells cannot. Your choice of production system may...of protein you need to produce.  The most common yeast strain used for recombinant antibody production ...Gervais C, Grazzini E, Durocher Y. Transient Gene Expression in Suspension HEK293-EBNA1 Cells. Methods Mol...PMID: 30242676. Frenzel A, Hust M, Schirrmann T. Expression of recombinant antibodies. Front Immunol. 2013...

  2. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...luciferase, cre/lox, yeast expression, worm expression, insect expression, plant expression, mouse targeting...(nematode), S. cerevisiae (budding yeast), S. pombe (fission yeast), A. thaliana (mustard weed), O. cuniculus...to be used. Choose from: mammalian expression, bacterial expression, lentiviral, retroviral, AAV, RNAi...

  3. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...and IT10 expressed well in cells and did not form undesirable aggregates. Using budding yeast as a model...GFP did not reduce yeast cell size or viability compared to untagged GFP overexpression, while the rest ...protein of interest, purify it, or determine its expression level without the need for a custom antibody....

  4. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    ...used for cloning in yeast since antibiotic resistance spontaneously arises in yeast. For example, Markus...Markus Ralser’s lab created a yeast prototrophy kit containing 23 plasmids to complement auxotrophy. Negative...used similarly to CcdB. For example, in the gene expression plasmid pGTvL1-SGC from Nicola Burgess-Brown’...

  5. Cre-ating New Methods for Site-specific Recombination in Drosophila

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    Blog Post
    .... When expressed in neuronal cells, each line showed broad recombinase-dependent RFP expression. Even ...problem is especially stark in Drosophila, where expression of Cre from the standard UAS/GAL4 system is toxic...sought to test the suitability of four additional yeast recombinases: KD (from Kluyveromyces drosophilarum...Even at low expression levels, recombination by KD, B2 and B3 resulted in complete or near-complete excision...of the mouse brain. Rubin’s work shows that the yeast recombinases KD, B2, B3 and FLP can be used together...

  6. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    ...Arg-mutant proteins in living baker’s yeast cells, including Arg-mutant yeast homologs of human proteins that...other disease-linked Arg-mutant proteins both in yeast and human cells in order to determine the specific...interactions and how these interactions regulation gene expression. Recent studies have shown that the conditions...will use plasmids from Addgene to construct NFIX expression vectors as well as reporters of NFIX function...interactions and how these interactions regulation gene expression.     ...

  7. New Podcast Segment: Hot Plasmids

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    Blog Post
    ...Constructs Kit from John Chodera's Lab - Get high expression of His-tagged human kinases in bactera. Listen...Kinase Domain Constructs Kit Hot Plasmid 4: The Yeast Prototrophy Kit from Markus Ralser's Lab -  Use ...Saccharomyces strains.  Listen to Our Segment on The Yeast Prototrophy Kit Special thanks to our sound engineer...

  8. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    ... be easily expressed in non-mammalian cells. Yeast expression plasmids The yeast expression constructs...S. cerevisiae expression vectors are available at Addgene. A set of mammalian expression vectors containing....  Bacterial expression plasmids For those constructs to be used in bacterial expression systems, Ginkgo...tag, cleavage, codon usage, and expression system. Mammalian expression plasmids Finally a set of wild ...Ginkgo synthesized SARS-CoV-2 expression plasmids in a high copy backbone with a T7 promoter driving multiple...all viral components are available in bacterial expression plasmids, glycoproteins and other proteins that...optimizations similar to those found in the bacterial expression plasmids. Each combination is present in two ...

  9. Hot Plasmids and Viral Preps - January 2021

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    Blog Post
    ...control expression of their genes of interest. While recombinases are powerful gene expression tools in...CRISPRa system in yeast is mediated by recruitment of MS2-functionalized gRNAs.  Find the yeast CRISPRa plasmids...inducible Cre for precise control of bacterial gene expression By Meghan Rego Recombinases are enzymes that ...conformational changes in a protein.    Cells expressing an RFP reporter plasmid for recombinase activity...large heterogeneous cultures.  HeLa cells expressed a fusion of keratin with mGold (top) and mVenus...SPOTlight was used to image over 3 million cell types expressing variants of Yellow Fluorescent Protein (YFP)....AAV8, as well as AAV5 and AAVrg. Find the Cre-expressing viral preps here AAV-CAG-dLight1.3b from the...

  10. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    ...switch to be expressed. In this case, the gene encodes the LacZ𝛼 protein. Once expressed, LacZ𝛼 complexes... RNAi is commonly achieved by feeding E. coli expressing dsRNA to C. elegans. These dsRNAs target specific... of long-time Addgene depositor Alice Ting used yeast display-based directed evolution to create APEX2...What’s more, since the enzymes were ”evolved” in yeast at 30℃, the enzymes function across a much broader...mutant animals that are genetically altered to overexpress endogenous RNAi pathways. These mutations however...

  11. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type
    Blog Post
    ...into an expression plasmid, the plasmid can be introduced into host cells, such as bacterial, yeast, or mammalian...Bradbury et al., 2018). Hybridomas can lose gene expression of the mAb-encoding genes or fail to revive after...vitro using synthetic genes that are typically expressed from a plasmid or from an integrated sequence ...identifying and cloning the genes for antibody expression. If starting from a protein sample (for instance...selection process is often performed via phage display, yeast display, ribosome display, or mammalian display ...antibodies are not monospecific: Hybridomas frequently express additional functional variable regions. mAbs 10...

  12. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    ...cloning and expression, as well as guide pages for Synthetic Biology and Bacterial Expression Systems. Check...you are looking for plasmids used in E. coli and yeast, you’re in luck! Addgene has so many of these resources...filters just for them on our search page. Use the Expression and Insert Species filters on the right-hand ...

  13. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...important if you are planning to express the FP in acidic environments (e.g. yeast cytosol, which is slightly...of these FPs are fluorescent when ectopically expressed in most organisms. Furthermore, fusing FPs to ... The two most common uses are: 1) to test the expression level in a specific system (by measuring the ...bacteria studies, whereas GFPS65T is better suited for yeast studies (24-30°C). Brightness: Brightness is a measure...He is interested in everything related to gene expression, particularly at the RNA level. He maintains ...proteins and imaging technologies to study gene expression, nuclear localization, and dynamics” Curr. Opin...

  14. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    Blog Post
    ...same strain. MazF expression is under control of the AOX1 promoter whose expression is induced by methanol...that are used to drive high protein expression in vivo. Expression plasmids are often lost without plasmid...to create unmarked genetic modifications in the yeast Pichia pastoris. By combining both positive and ...hok/sok system to create strains that can stably express antigens in sufficient quantities to generate an... genetic modification of Pichia pastoris." FEMS yeast research 9.4 (2009): 600-609. PubMed PMID: 19416369...plasmid R1: Sok antisense RNA regulates hok gene expression indirectly through the overlapping mok gene."...James B. Kaper. "Use of stabilized luciferase-expressing plasmids to examine in vivo-induced promoters...

  15. An Integrin Antibody Toolkit from IPI

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    Blog Post
    ... have proved challenging to study. Cells often express multiple integrins that bind the same ligand, making...antibodies (rAbs) either produced de novo using yeast display technology or produced from hybridoma antibodies...demand. The 11 IPI synthetic rAbs, developed using yeast display technology, are directed to integrins that...combination, showing that among the three αV integrins expressed on the BJ-5ta fibroblast cells, integrin αVβ1 ...2024). Synthetic integrin antibodies discovered by yeast display reveal αV subunit pairing preferences with...

  16. Easing the Protein Purification Process with pCri

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    Blog Post
    ... information, you can then pick an expression vector and expression system. If you find that your protein...special E. coli strains designed for protein expression. Cultures are then lysed and the protein of interest...stability and solubility. Determining the best way to express one’s protein of interest can save a lot of time...pCri also includes plasmids designed for protein expression in systems other than E. coli, specifically the... the bacterium Bacillus subtilis and the yeast Pichia pastoris. The improved secretory capacities of these... and disulfide bond formation. If one prefers expression in E. coli, the use of a thioredoxin A (TRX)-...tagged proteins into the membrane and may improve expression of proteins that natively localize there. pCri...

  17. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ... million proteins per cubic micron in bacteria, yeast, and mammalian cells (Milo, 2013). The number of...capture protein complexes, scientists can simply express a bait protein fused to BirA* in cells and then...sensitivity. Sensitivity could be increased with higher expression levels but for many fusion proteins this would... circumvent these issues, the Ting lab utilized yeast display-based directed evolution to generate two...resources on the Addgene blog Plasmid 101 Protein Expression Plasmid 101 Biotinylation Other resources Proximity...

  18. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...fundamentally the reporter construct monitors the expression of a nearby gene. The reporter gene serves as...as a proxy for the endogenous gene – its expression levels (high/low) and patterns (tissue/timing) can...that the gene which GFP was monitoring was being expressed in the same pattern. These systems suggest the...historically been used extensively in fly, mouse, and yeast models. Why traps are useful Traditional genetic...function of a gene. For example, they eliminate the expression of the gene (by CRISPR knockout, shRNA depletion...Tissue-specific genes – the genes of interest may not be expressed in the tissue/cell line you are working with. ...the gene as a result but will still allow for expression pattern monitoring. Enhancer traps Like promoter...

  19. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ... single protein is expressed, with the intent of purification, it will be expressed along with all other...other cellular proteins within the expression system (bacteria, yeast, mammalian, etc.). To individually...about that protein that no other protein in the expression system has. This is where affinity tags come ...peptides which do not naturally exist in most expression systems that can be added to the N or C terminus...on: The protein you are trying to purify The expression system If you want the tag to remain on your ...MBP tag, although large, can actually increase expression and solubility of the tagged protein (Fox and...Enhancer. In P. E. Vaillancourt, E. coli Gene Expression Protocols (Vol. 205, pp. 99–118). Humana Press...

  20. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...plasmids for mammalian systems, bacteria, plants, and yeast. Popular cytosine base editor pCMV-BE3 has been ... probably explains why pC016 - LwCas13a guide expression backbone with U6 promoter has already earned ...Day 5: Delivery systems: CRISPR 101: Mammalian Expression Systems and Delivery Methods and A Match Made...Multiplexing and Cas9 variants: CRISPR 101: Multiplex Expression of gRNAs and The PAM Requirement and Expanding...regulation: Truncated gRNAs for Regulating Gene Expression and CRISPR 101: Epigenetics and Editing the Epigenome...
Showing: 21 - 40 of 130 results