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We narrowed to 1,010 results for: RING;

Showing: 191 - 200 of 1010 results

  1. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...Kleckner. "Recombination, pairing, and synapsis of homologs during meiosis." Cold Spring Harbor perspectives...generate double-mutant offspring. Crossing mice allows for the production of offspring that have a combination...generate a desired phenotype Instead, genetic engineering should be used to separately introduce each mutation...modification will be passed along to all of the offspring in the F1 generation, your breeding scheme should... two mutations that you want combined in your offspring. Genotyping involves getting a small piece of ...is homozygous for a different mutation, the F1 offspring will be either hemizygous or null at one locus...mice that were bred in your colony, as opposed to ordering control mice from a stock center. This serves ...

  2. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...tools available for monitoring how both the brain and visceral organs interact during various behaviors.... This is all an impressive engineering feat, but you’re probably wondering “did it actually work”? The...Neuromodulation During Free Behavior. 2020 42nd Annual International Conference of the IEEE Engineering in Medicine...continuously monitor it. How to address this? Through the pairing of materials scientists (Anikeeva lab) with gut-brain...physically hooked up to invasive, cumbersome systems during experiments – neither ideal for the intrepid researcher... fiber (Park et al., 2019). This provided an engineering road map of sorts, giving an idea of the stiffness...affect their function (the gut twists and contorts during the digestion of food, while brain tissue is relatively...

  3. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ... professor or hiring manager. Otherwise, use a gender-neutral greeting like “Dear Hiring Manager.” Highlight...If you are job searching, you may be wondering if you need to go to the effort of writing a cover letter...requires ddPCR skills. Your resume lists ddPCR, so the hiring manager knows that you have ddPCR experience. In...assay to answer an experimental question. Now, the hiring manager knows that you can design experiments with...briefly explaining a gap in your job history or sharing relocation plans. They can be skills-based: if ... of the department] at [name of the university].During my thesis work on [phenomenon X], I read several...joining your team as a postdoctoral researcher. Dear Hiring Manager, I am writing to apply for [title of the...

  4. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...of “bringing tomorrow’s science into today’s classrooms,” BioBuilder answers the call by offering a variety...Tamer from BioBuilder, an innovative nonprofit bringing the study of synthetic biology into the hands ... Kuldell from MIT’s Department of Biological Engineering. Built primarily from her 20 years of college... boost interest and understanding around the engineering of biology. Participating schools are provided...BioBuilder inspires students and teachers to apply engineering principles to biological systems. Today, what...BioBuilder module allows students to explore how engineering fields rely on rapid prototyping to develop and...high school teams around the world who combine engineering approaches and scientific know-how to design/...

  5. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...and scientists - Culturing a scientific network We refer to the process of mentoring within a bin as Horizontal...and more. We are partnering with ASM and are working together with the society to bring an even more valuable...next component of Binning Singletons is when we bring all the bins together at a mixer. We call this Quorum...also sought expertise from many with experience mentoring in STEM, including Addgene’s Joanne Kamens. Expanding...Chicago.  Finding our place in networking and mentoring circles We looked at a lot of approaches scientific...scientific meetings have taken to provide mentoring. For example, Goldschmidt conference, Coastal & Estuarine...historically underrepresented groups. Through such mentoring programs, scientists can build and expand their...

  6. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...28060411. Thomason, Lynn C., et al. "Recombineering: genetic engineering in bacteria using homologous recombination...leaves a “scar” in the chromosome, sometimes interfering with expression of surrounding genes. However...Developed over the past 20 years, Lambda Red recombineering, which uses enzymes domesticated from the Lambda...enzymes, require multiple transformation and plasmid curing steps, and can generate off-target mutations. ...of bacterial toxins to facilitate efficient recombineering (Khetrapal et al., 2015). They created vectors...counter-selection which enabled very efficient recombineering.     We were inspired by this work and sought...16568824. Hmelo, Laura R., et al. "Precision-engineering the Pseudomonas aeruginosa genome with two-step...

  7. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ... IPI and the Springer lab (Hao et al., 2024). In this study, we, along with the Springer lab, reported...stabilizes the extended open integrin conformation. During embryonic development, extended-open integrins ...development, and the shaping of the extracellular matrix. During immune responses, this same conformation encourages...produced from hybridoma antibodies developed in the Springer lab. Some are rabbit immunoglobulin G1 (IgG1) ... to be developed and defined. According to the Springer lab, a highly specific function-blocking antibody...   Targeting integrins in the lab The Ha and Springer labs recently showed RGD-binding integrins demanded...integrins. The Ha lab — in collaboration with the Springer lab, the Jaumouillé lab, the Waterman lab, and...

  8. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...As Addgene believes many of the topics discussed during the symposium will prove incredibly important to... that the main question they wanted to focus on during the symposium was “How many graduate students/post-docs...problems in academic research and potential solutions During the first couple of sessions of the symposium it...In fact it is this very idea that postdocs are acquiring training to become professors that is provided...estimates that postdocs are paid $16.15/h before fringe benefits, less even than the $21/h [on the low ...low end] that grad students are paid before fringe benefits); what they don’t make in wages, they make up...programs as the standard option for scientists by requiring graduate students to pay for part of their tuition...

  9. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    ...cooking competition featuring a selected ingredient. Anyone who wants to participate brings in a dish that .... The four of us would eat together every day, sharing ideas for the company, but also talking about sports...a culture of openness and trust that comes from caring about your colleagues on a personal level, and ...special occasions. However, employees regularly bring in baked goods and we have a few unique lunchtime... from the software folks and started Hack Days. During Hack Days, teams at Addgene will forgo their regular...website. Free lunch is provided for participants during the Hack Days, and we’ve seen great success at ...university departments to establish, with the goal of fostering better communication between labs. Scientists ...

  10. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ...naturally occurring Cas9 diversity via the Integral Microbial Genomes (IMG) database, Harrington et al discovered...are available from Addgene. Happy CRISPRing! References Harrington, Lucas, et al. "A thermostable Cas9...Yes Longevity in Serum?* No Yes Table 1. Comparing SpCas9 and GeoCas9. *For in vitro Cas9 RNP activity...temperatures much warmer than SpCas9 can tolerate. Harrington et al tested GeoCas9’s love of heat in two ways...can introduce genomic edits in mammalian cells. Harrington et al tested GeoCas9’s editing skills by transfecting...had similar levels of editing. See figure 4 in Harrington et al for more examples of GeoCas9’s editing ...sleeve is its increased longevity in human serum. Harrington et al suspected GeoCas9 might have higher stability...
Showing: 191 - 200 of 1010 results