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We narrowed to 284 results for: cas9 plasmid

Showing: 191 - 200 of 284 results

  1. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...SnapGene powered plasmid maps at Addgene  Three factors make our SnapGene powered plasmid maps far easier...easier for you to share plasmid information with colleagues, or explain plasmid features to a lab member... on the main plasmid page under the "Resource Information" section. Analyze your plasmid sequence For ...over from our users: "Please, please improve your plasmid maps!" After thoughtful design, vetting, and tweaking...tweaking, we’re excited to announce that our plasmid and sequence displays are now powered by GSL Biotech's...software and extensive feature library, our updated plasmid and sequence displays are now much easier to interpret...how they’ll make it easier for you to find the plasmids you need. We'll be monitoring and improving the...

  2. Zebrafish Plasmid Collection

    Type
    Collection
    ... Plasmid Collections Zebrafish Plasmids Zebrafish Plasmids and Resources... of the plasmids and tools available at Addgene for use in zebrafish. Browse Plasmids Plasmid Tools for...for plasmids containing your zebrafish gene of interest? Search Addgene's collection for plasmids that...highlights plasmids that contain tools for use in zebrafish research. This includes plasmids for genome...and Cre expression plasmids. Large collections or kits are described below . ID Plasmid Description Gene...CRISPR resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples...Using CRISPR/Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression plasmids - Keith Joung...

  3. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...need Cas9 plasmid Chen and Wente 42250 DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid Joung...Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids that have been designed for use in zebrafish. CRISPR... CRISPR Zebrafish CRISPR Plasmids: Zebrafish...Zebrafish Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional ...Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed for use in zebrafish. Cut Fully...

  4. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...Resources on the Addgene Blog: Plasmids 101: The Promoter Region Plasmids 101: Cre-lox Light-Switchable...Promoter Subtype Promoter Example Activator Addgene Plasmid/Kit Alcohol-regulated AlcA promoter AlcR pGGA008...depositors have developed heat shock-inducible Cre and Cas9 for easy genome engineering in species like C. elegans...use light to regulate transcription. Red flame plasmid pDawn contains the blue-light sensing protein YFI...Systems for E. coli Browse other blog posts from our Plasmids 101 series Resources on Addgene.org: Inducible...Expression Systems at Addgene Cre-lox Guide and Plasmids     ...

  5. Important Considerations When Using AAVs

    Type
    Blog Post
    ...Currently, AAVs are being used to deliver CRISPR/Cas9 but have the potential to deliver other therapeutic...used with new exciting technologies (such as CRISPR/Cas9) as they allow for gene delivery in an efficient...Expression in Mammals A Match Made in Heaven: CRISPR/Cas9 and AAV Viral Vector Elements Additional Resources...Ready-to-Use AAV Preps Browse All of Our Viral Plasmids and Resources ...

  6. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ...), 339-350 (2015). PubMed. Find the Plasmids @Addgene: Plasmids for CRISPRi are described here: http...) model consists of a catalytically dead Cas9 protein (dCas9) that lacks endonuclease activity coupled...constructs to be introduced simultaneously in a single plasmid. The functionality of the RNA scaffolds follows...http://www.addgene.org/crispr/qi/ Plasmids described in  Zalatan et al. Cell  (2015) can be found here...https://www.addgene.org/browse/article/9593/ Plasmids described in Qi et al. Cell  (2013) can be found.... Using dCas9 as an on/off switch The multiple scRNAs all individually utilize the same dCas9 protein....modulate dCas9 expression, multiple scRNA transcriptional programs can be regulated with dCas9 as a master...

  7. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ...CAPTURE 2.0 to be used with primary cells. Find plasmids for CAPTURE 2.0! When compared to the original...1st time CRISPR users Learn how to Choose the Best Cas9 Variant for Your Next Experiment Learn More About... Additional Resources on Addgene.org Find Other Cas9 Variants for Purifying Genomic Regions of Interest...co-expressed, the gRNA targets the dCas9 to the loci of interest and dCas9 is biotinylated by BirA. From here...To increase the resolution, the Xu lab created a dCas9-based CAPTURE (CRISPR Affinity Purification in situ... cell line that co-expresses three components: dCas9 with an added biotin acceptor site BirA, a biotin...be pulled down via streptavidin IP. Biotinylated dCas9 goes along for the ride throughout all of these ...

  8. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...from single or multiplex CRISPR-Cas9 double-strand DNA breaks using SpCas9. More features will be added ...Currently, ICE is configured for Cas9 fromStreptococcus pyogenes (SpCas9). ICE Score - The editing efficiency...Addgene.org Check out our CRISPR guide Find CRISPR plasmids for your research Find other CRISPR software resources...Addgene Blog Browse our CRISPR featured topic page xCas9: Engineering a CRISPR Variant with Pam Flexibility...

  9. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...over 100 requests for the plasmids and the lab eventually deposited these plasmids at Addgene. While deposits...The mission to share critical reagents such as plasmids and viral stocks for researchers around the world...Therapeutics and is an Addgene depositor. KSQ uses a CRISPR-Cas9 platform to systematically test the function of ...

  10. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...used with many types of proteins, including Cas9 and dCas9-transcription factors, for which low expression...light-responsive system is spread across three plasmids, with the response regulator encoded in the genome...et al. condensed the system to two streamlined plasmids. The utility of these TCSs was limited by a few...These bulky systems were spread over a number of plasmids, with one RR (ompR) encoded chromosomally. Leakiness...constructs have been greatly streamlined; only two plasmids are required per system. Applications of these...many strains of E. coli, as well as with other plasmid-based systems, which will enable the assembly of...sb500273n. Epub 2014 Oct 11. PubMed. Find the plasmids from this publication at Addgene. Synthetic biology...
Showing: 191 - 200 of 284 results