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We narrowed to 885 results for: TRI

Showing: 381 - 400 of 885 results

  1. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ... use the material in human subjects or clinical trials Acknowledgement in recipient’s future publications... benefits, we know that legal documents can get tricky. Here’s how Addgene helps: For Providers: One-time... FREE!) and we’ll take it from there. We will distribute your materials under the UBMTA terms, so you ...

  2. Editor's Choice, August 2016

    Type
    Blog Post
    ...difficulty with NgAgo, but a few successes have trickled into the survey. In response to a bit of a Twitter...to make single base edits in DNA. The first, contributed by guest blogger Kristian Laursen from Cornell...mutated plasmid constructs. The second post was contributed by Addgene Scientist Mary Gearing and discusses...

  3. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    ...The following post was contributed by Daniel Bauer and Matthew Canver of Boston Children’s Hospital and...frameshift mutations. However these may require restriction fragment length polymorphism analysis, allele-specific... you to our guest bloggers! Daniel Bauer is a pediatric hematologist and principal investigator at Boston...

  4. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...Yeast. 1998 Jul;14(10):953-61. Note: Marcy Patrick contributed to the writing of this article....isolation of successful transformants. Addgene distributes several ready-to-use, modular plasmids, combining...

  6. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ... a repair template, using Gibson Assembly or restriction enzyme cloning. Streptomyces bacteria are more... presence of just a single organism, such as Clostridium difficile, could be targeted and preferentially...base editing in Streptomyces Some of the most industrially and clinically relevant bacterial species reside...CM, Ousterout DG (2020) In Vivo Targeting of Clostridioides difficile Using Phage-Delivered CRISPR-Cas3...

  7. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ...Recycle plastic packaging Packaging is a huge contributor to the increase in plastic manufacturing, accounting...accept and to print a free shipping label. Recycle nitrile gloves Terracycle is a company that takes items... a box with the prepaid label and fill it with nitrile gloves, affix the prepaid label, and divert these...

  8. An Addgene Summer Internship

    Type
    Blog Post
    ...heavy restrictions on what I could and couldn’t do as an intern. I expected that these restrictions would...choose what gene to focus on. The gene choice had restrictions. It couldn’t be a Covid gene, a toxin, or an...

  10. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ... such as Stbl3s. ITR loss can be detected by restriction digest using enzymes, like SmaI, which have recognition...organism or tissue type-specific, which can help you restrict the expression of your transgene to a specific...result is a permanently flipped transgene. By restricting the availability of Cre, lox sites lets researchers...

  12. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...Minigenomes - A Safe Way to Study Dangerous Viruses Contributed by guest blogger Tessa Cressy from the labs of...leaving virulent components behind. Minigenomes are stripped down viral genomes containing only the sequences...fantastic depositors and guest writers. Your contributions make Addgene and the Addgene Blog useful resources...

  14. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...This post was contributed by guest bloggers Samantha Zyontz and Neil Thompson from the MIT Sloan School...thanks to Addgenies Pamela McA'Nulty and Zachary Tribbett for helping compile the data used in this work..., computing performance, and randomized control trials in innovation. Visit his website at: http://www.neil-t.com...

  15. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ... with biorender.com. ITR structure  ITRs are symmetrical 145-nucleotide sequences that flank the ends ...identification of non-dominant ITR sequences.  Restriction digests with the enzymes SmaI and SrfI can also...deletions and point mutations will go undetected by restriction digest methods.    Resources and references...

  16. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ... research community. At Addgene, we’ve helped distribute many of these technologies in plasmid form and...libraries. Please note, the pooled libraries we distribute in lentivirus format only deliver gRNA and therefore...like to thank all those who are allowing us to distribute their CRISPR tools as ready-to-use virus and,...

  20. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...your antibody.  At some point in your long and illustrious lab career, you will likely accidentally leave...Brightness Chart. Boston University Flow Core. Retrieved January 31, 2022, from https://www.bu.edu/flow-cytometry...overlap. Syracuse University Flow Core Facility. Retrieved January 31, 2022, from https://flowcore.syr.edu...
Showing: 381 - 400 of 885 results