We narrowed to 910 results for: ire;
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TypeBlog Post...decision. We realize that with this move, we lose a direct connection with our nearly 22,000 X followers. ...official Addgene account. You can reach out to us directly via email at [email protected], and we invite you...
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Addgene's 20th Anniversary Party
TypeBlog Post...and CSO Melina Fan's words of welcome! Executive Director Chonnettia Jones and Board Member Joanna Brownstein...each and every Addgenie, from our co-ops to our directors, we can't celebrate Addgene without recognizing... -
Celebrating the 2024 Nobel Prize in Chemistry
TypeBlog Post...John Jumper! The Baker Lab focuses on designing entirely new proteins, improving our understanding of how...John Jumper! References Kuhlman, B., Dantas, G., Ireton, G. C., Varani, G., Stoddard, B. L., & Baker, D... -
CRISPR Cheat Sheet
TypeBlog Post...Components of CRISPR: gRNAs: Sequences of RNA that direct the CRISPR system to cut other DNA or RNA sequences...Nucleases: Proteins that bind to gRNAs and are directed by the gRNAs to cut particular DNA or RNA sequences... -
Summer Fun at Addgene!
TypeBlog Post...around the corner. Here at Addgene, we’re always inspired by the new seasons, so we've made some big summer...Landscape for Engineered Systemic AAVs” with the director of CalTech’s CLOVER lab, Tim Miles, PhD, we invite... -
Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More
TypeBlog Post...Collins labs at the Wyss institute for Biologically Inspired Engineering. Alex is the first MD PhD we've had... - 13:21 : The Wyss Institute for Biologically Inspired Engineering and why Alex decided to work there... -
2018 #DeckTheLab Contest Winners
TypeBlog Post...Matthew J. Sikora (@mjsikora) December 18, 2018 Fireplace from the Dyer Lab If you’re feeling as chilly ..., they turned one of the lab computers into a fireplace scene. Got our @EmoryChem lab office looking... -
Celebrating One Million Plasmids Shared!
TypeBlog Post...would be an average of 673 plasmids each day. This requires tremendous coordination amongst the lab team, ...above), but as Joanne Kamens, Addgene’s executive director, said during our celebratory lunch, it’s hard ... -
AAV Packaged on Request is Here!
TypeBlog Post...below the ordering table. Click on it, select your desired serotype and volume, and submit your request. ...simply request your plasmid of interest in your desired serotype, place your order when ready, and we’ll... -
Editor's Choice, October 2016
TypeBlog Post...Increased Postdoc Wages,” Future of Research executive director, Gary McDowell, gives a thorough yet concise rundown...postdocs various academic institutions can afford to hire. It will be difficult for some of these institutions... -
Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs
TypeBlog Post...biomass. Dr. Duedu would like to use the plasmids he acquires through Addgene to produce molecular biological...PCR. We hope these plasmids will help Dr. Duedu inspire new Ghanaian scientists to continue to do excellent... -
Plasmids 101: How to Name Your Plasmid in 3 Easy Steps
TypeBlog Post...insert are often denoted by "wt" either directly before or directly after the insert name. Example: If ... -
Synthetic Biology, Artificial Chromosomes, iGEM & More - Interview with Tom Ellis
TypeBlog Post...Cambridge before spending time in biotech screening direct interactions between drugs and promoters of oncogenes...and extremophiles for biotechnology and has been directly involved in supervising many successful iGEM undergraduate... -
MassBioEd Career Exploration Day at Addgene
TypeBlog Post... an introduction to Addgene with our Executive Director, Joanne Kamens. As a regular contributor of careers...teams at Addgene. An introduction to plasmids with Director of Biology Lianna Swanson was very popular, with... -
Fueled by Coffee at #SfN14
TypeBlog Post...cups of coffee would be consumed throughout the entire 5 day event. Though, to be fair, I think this is... a day, and I had colleagues who were much more wired. Here at #SfN14 there are thousands of posters being... -
CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping
TypeBlog Post...sequence of ssDNA. Unlike Cas9, Cas14 does not require a PAM sequence. In addition to this cis cleavage...Cas12 are for the other types of nucleic acids and required a certain sequence specificity in the middle of... -
Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies
TypeBlog Post...about what's out there.”- Joanne Kamens, Executive Director Career exploration “You can't wait for opportunities...my team at Addgene was created.”- Eric Perkins, Director of Science Product Management “I knew very early... -
Your Top Requested Plasmid in 2016!
TypeBlog Post...target sites 5' of the 5'NGG3' PAM sequence as directed by a gRNA. 2A-Puro In this plasmid, SpCas9 is ...stand breaks and may better facilitate homology directed repair. You can find all of these variants, as... -
Why Do I Need an MTA Anyway?
TypeBlog Post..., and almost all universities and institutions require them for material transfers. When Addgene entered...and emails it straight to you, or we can send it directly to your institution’s MTA coordinator. Organizations... -
The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog Post...still gets requested! Fire Lab C. Elegans Kit - Our first popular "kit," Andrew Fire's set of vectors for... -
Top Requested AAV of 2017: pmSyn1-EBFP-CRE
TypeBlog Post...otherwise ubiquitous promoter. You could make an entirely new mouse line with mCherry controlled by a neuron...gene of interest in neurons. This ability to be paired with any Cre-controlled gene of interest makes ... -
Editor's Choice, September 2016
TypeBlog Post...smartphone apps you can use in the lab. Whether you’re tired of calculating dilutions or you can’t quite remember...contributed by Kurt Swanson, husband of Addgene Director of Biology, Lianna Swanson, and researcher at ... -
CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats
TypeBlog Post...member of the Addgene Advisory Board, and Associate Director of the Genetic Perturbation Platform at the Broad...blogger, John Doench! John Doench is the Associate Director of the Genetic Perturbation Platform at the Broad... -
SciComm with the Experts at Science in the News Part 2
TypeBlog Post...graduate student at Harvard University, former Co-director of Science in the News, and member of the SITN...her PhD from Harvard University, is a former Co-director of Science in the News, and is a member of the... -
Science as Art
TypeBlog Post...of newspapers. Science is now being adapted to inspire to a new generation. Daniel Stoupin is a PhD scientist...The other thing photography has shown to me - it requires an awful amount of skill, time for which most... -
Three Tips to Organize Your Lab Notebooks in the New Year
TypeBlog Post...smartphone. Evernote allows you to create notes directly from webpage clippings, photographs, and file ... to the necessary time and resource investment required to change the status quo. But with recent improvements... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog Post... been difficult to study without biosensors to directly visualize their activity. In 2019, the first generation..., Wu, Z., Wu, H., Chen, W., Zhang, P., Zou, J., Hires, S. A., Zhu, J. J., Cui, G., Lin, D., Du, J., & ... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog Post...is key: due to differences in the PAM sequences required by each ortholog, a gRNA designed for one dCas9...fluorescently labeled dCas9 isoforms are efficiently directed to the proper target sequence. They were also ... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog Post...RNAi, the field for which Craig Mello and Andrew Fire won the Nobel Prize. What did Dr. Doudna think was...gRNA and crRNA could be combined into one RNA to direct cleavage the result was a simpler 1 protein - 1... -
Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!
TypeBlog Post...acids are essential, which means that the body requires them but cannot produce them. By using yeast integration...aims to leverage a glycine oxidase reaction to indirectly detect glyphosate via H2O2, one of the reaction...