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  1. Resume Writing for Non-academic Science Careers

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    Blog Post
    ...need to present yourself using a resume. Indeed, instead of having all the pages in a Curricula Vitae to...career, she transitioned into a communications role instead after she completed her MSc in Experimental Medicine...
  2. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...vector developed by Feng Zhang’s lab at the Broad Institute (Sanjana et al., 2014). It is a dual-vector system...viruses are popular, pAAV-hSyn-DIO-hM4D(Gi)-mCherry contains a double floxed human muscarinic receptor 4 (Gαi-coupled...
  3. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    .... Projects such as the OpenFlexure Microscope (Collins, et. al., 2020), the miCube, SQUID (Hongquan, et...and low-cost optical setups. References Joel T. Collins, Joe Knapper, Julian Stirling, et. al, "Robotic...
  4. Building and Validating Recombinant Antibodies: A Case Study

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    Blog Post
    ... coexpression of light (green) and heavy (blue) chains as driven by two CMV promoters (orange). Figure... to WGS the lab’s entire hybridoma collection. Instead, the Trimmer Lab moved to direct functional validation...
  5. Sweating the Small Stuff: Details in the Lab

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    Blog Post
    ...for your workflow. Next time you make a mistake, instead of silently vowing never to do that again (I’ve...incubation periods to have another short "quiet period" instead of immediately running to the next thing.  It may...
  6. Antibodies 101: Buffers, Storage, and Conjugates

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    Blog Post
    ...buffer components could affect the reaction. For instance, sodium azide, a common antimicrobial agent added...relatively rare protein in an experiment with many proteins of interest! Research the brightness of your fluorophores...
  7. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...communicates your interest in a specific program, and institution. Consequently it’s best to draft a cover letter...or mentor. Several days prior to the interview, inspect your technology, background, and lighting for a...
  8. New and Upcoming Viral Vectors - May 2020

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    Blog Post
    ...months: Lentiviral preps for expressing SARS-CoV-2 proteins ChR2 for targeted expression DREADDs GFP dependent... quickly shared plasmids expressing SARS-CoV-2 proteins at the beginning of April. Now, we've turned some...
  9. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    Blog Post
    ...gene knockout in vivo and in vitro, as well as to insert targeted mutations through homologous recombination...effects and/or toxicity, it would be advantageous to instead use a preexisting, conserved cellular machinery...
  10. Dos & Don'ts When Publishing a Scientific Manuscript

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    Blog Post
    ...journal. An increasing number of funding bodies insist that the research data resulting from their funding...many journals use to verify whether a manuscript contains all the necessary information to be of maximal...
  11. Addgene: The First Twenty Years

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    Blog Post
    ...biomedical researcher at Lunenfeld-Tanenbaum Research Institute, posting about Addgene’s 20th anniversary on Twitter...alike. We transformed this process by aligning institutions with standardized agreements and electronic ...
  12. Tips for Technical Support Calls

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    Blog Post
    ...last digit: An answer should come soon, but not instantly. Addgene tries to get back to customers within... Eric J. Perkins...
  13. Plasmids 101: Modular Cloning

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    Blog Post
    ...example: in a cell there are proteins which interact with other proteins and with DNA, and these complexes...
  14. Viral Vectors 101: Chemogenetics

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    Blog Post
    .... The advantages provided by chemogenetics are instead that it is noninvasive and has longer-lasting effects.... PSAMs themselves are modified ligand binding domains. When coupled to the ion pore domain (IPD) of a...
  15. Plasmids 101: FLEx Vectors

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    Blog Post
    ... now specifically drives expression of mCherry instead of BFP. Beyond switching fluorophores, why is FLEx...optogenetic reporter would ensure that the reporter remains silent until a cell or tissue-specific Cre is provided...
  16. Hot Plasmids and Viral Preps - January 2021

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    Blog Post
    ...in a variety of outcomes including excision or insertion, inversion, translocation or cassette exchange...addition, Casɸ has a minimal PAM requirement. For instance, for Casɸ-2 the PAM sequence is 5’-TBN-3’ (where...
  17. How to Prepare for an Industry Interview

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    Blog Post
    ... a STAR answer once the interview has started. Instead, I preferred to prepare stories to answer interview...possible. Otherwise, read the video conferencing instructions you received from HR carefully. And even if ...
  18. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...widely used method due to the accessibility of instruments in standard labs. When determining titers by ...., Pawlita, M., Ferrari, F., Samulski, R., & Kleinschmidt, J. (1999). Titration of AAV-2 particles via...
  19. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...questions. Practice your presentation for both instances. If your poster material is nearing publication...Connect with people on LinkedIn or Twitter, for instance. If you’ve had a particularly meaningful conversation...
  20. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...transcription and chimeric efforts make a precise edit or insertion via a chemical modifier. We used these categories... plasmids seem to never truly go out of style. Instead, an almost even mix of plasmids (by age) are requested...
  21. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...light to distinguish one fluorophore from another. Instead of using a single (primary) detector to identify...narrow band, to discern one marker from another. Instead of compensation, which relies on user adjustment...
  22. Polymerase Chain Reaction Overview and Applications

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    Blog Post
    ...plasmid of interest, for detecting the presence of an insert or piece of DNA in a sample such as in colony PCR...into approximately 20,000 droplets. Each droplet contains the material required for PCR amplification. Following...
  23. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...time to produce beta-lactamase prior to division, instead of immediately experiencing the effects of the ... capable of correctly folding disulfide bonded proteins in its cytoplasm. Microbial Cell Factories, 11...
  24. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...information. Personally, I would much rather read the instructions than watch a how-to video. My dad, on the other...love it and others will avoid it like the plague. Instead of looking for The One Method to Rule Them All,...
  25. A Guide to Starting Your Own Journal Club

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    Blog Post
    ...including casual request via email, in person, or instant messaging but we as scientists often prefer data...failing in many academic journal clubs where institutional inertia drives the meeting rather than interest...
  26. Negotiating Work and Life: How to Find the Joy

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    Blog Post
    ...run and the long run and so will your work. Nobody wins, and you need to make a change. #2 Thou shalt sometimes...tasks – try to resist the impulse to control by insisting things always be done your way. Be patient with...
  27. Deciphering the Mysteries of Behavior with Viral Vectors

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    Blog Post
    ...open new ways for a novel therapeutic approach against drug addiction. Anxiety tests Rodents have a natural...are ahead for behavioral neuroscience to reveal insights into the complex relationship between genes, neurons...
  28. New Tools Enable CRISPRa for Neuroscience Applications

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    Blog Post
    ...is not amenable to FACS.  Thus, the researchers inserted human CD8, a gene that encodes a surface protein...yourself whether gene overexpression is necessary (instead of gene knockout or knockdown, for which there ...
  29. Lab to Office Culture Shock

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    Blog Post
    ...for scientists making this transition. In this installment of the Addgene career guide, we’ll think about... Eric J. Perkins...
  30. Viral Vectors 101: Preparing Pooled Libraries

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    Blog Post
    ...you can either order a DNA pooled library, which contains a collection of plasmids in a single tube, or ... CRISPR libraries. Protocols from the Broad Institute: A variety of protocols and guides regarding pooled...
  31. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...Livny J, Regev A, Koonin EV, Hung DT, Sabeti PC, Collins JJ, Zhang F (2017) Nucleic acid detection with ...Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang F (2018) Multiplexed and portable nucleic...
Showing: 761 - 800 of 1055 results