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TypeBlog Post...days or weeks), look over your notes and protocol(s). Is there enough information there for you to be ...
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Viral Vectors 101: Preparing Pooled Libraries
TypeBlog Post...10.1002/cpmb.8. PMID: 27366891. Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD... -
Getting to Know Expansion Microscopy
TypeBlog Post...992. https://doi.org/10.1038/nbt.3625 Truckenbrodt S, Sommer C, Rizzoli SO, Danzl JG (2019) A practical... -
Bioinformatics at Addgene
TypeBlog Post...sequential computational tasks for which existing script(s) have been developed for a singular purpose (ex. adapter... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog Post...Kadina, Adrianna E. Zepeda, Kevin Holden, Jonathan S. Gootenberg, Omar O. Abudayyeh. Drag-and-drop genome... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog Post...broadly one can interpret our analysis of COVID-19’s effect on scientific activity. The effects of COVID... -
DNA Purification Without a Kit
TypeBlog Post... Y., He, W., Wang, J., Peng, F., Huang, L., Zhao, S., & Deng, W. (2018). Rapid Regeneration and Reuse ... -
Plasmids 101: Control Plasmids
TypeBlog Post...decreased expression of Gene X. Ergo the positive control(s) should decrease the expression of Gene X. Since the... -
Tips for Using FRET in Your Experiments
TypeBlog Post.... 2007 Sep; 32(9): 407-14. Zimmermann T, Terjung S. FRET Basics and Applications: An EAMNET Teaching ... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog Post...interest will be expressed. The RNA polymerase(s) RNA is transcribed from DNA using an RNA polymerase... -
Negotiating Work and Life: How to Find the Joy
TypeBlog Post... not to do this. For a family with working parent(s), the sooner your kids can make a decent meal, fold... -
Troubleshooting and Optimizing a Western Blot
TypeBlog Post...trouble by confirming your transfer worked with Ponceau S staining or other reversible protein staining immediately...resources References Luo, H., Rankin, G., Straley, S., & Chen, Y. (2011). Prolonged Incubation and Stacked... -
QC Sequencing Technologies at Addgene
TypeBlog Post...//doi.org/10.1021/ac2010857 Sanger, F., Nicklen, S., & Coulson, A. R. (1977). DNA sequencing with chain-terminating... -
Hot Plasmids Spring 2024
TypeBlog Post...many closely-packed labeled cells. Plus, Voltron2’s fluorescence is tunable by changing the dye that becomes... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog Post...Many thanks to our guest blogger Gary McDowell! Gary S. McDowell is currently the Executive Director of Future... -
Site Directed Mutagenesis by PCR
TypeBlog Post...minor extension can usually ensure that the 3’-base(s) do not form secondary structures. The introduction... -
Transferable Skills Guide: Creativity
TypeBlog Post...mental-mistakes-that-kill-your-productivity Finkelstein, S. (2019, July 15). A brief exercise to spur innovation... -
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog Post...Foronda M, Zimmerman J, Diaz B, Zafra MP, Goswami S, Dow LE (2020) GO: a functional reporter system to... -
Plasmids 101: Plasmid Incompatibility
TypeBlog Post...2958.2005.04643.x Pubmed PMID: 15916596 Kamruzzaman M, Shoma S, Thomas CM, Partridge SR, Iredell JR (2017) Plasmid... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog Post... tactic, and puts you ahead of most start-up P.I.’s. It doesn’t have to be complicated, or difficult to...