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TypeBlog Post...sequential computational tasks for which existing script(s) have been developed for a singular purpose (ex. adapter...
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Hot Plasmids - November 2023
TypeBlog Post... Find PE6 plasmids here! Doman, J.L., Pandey, S., et al. (2023). Phage-assisted evolution and protein... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog Post...the pre-designed primers to determine which version(s) of the capsid gene successfully infected the cell... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post... --very-sensitive-local -L 9 -N 1 -U GRNA_23MERS -S GRNA_HITS.sam Many thanks to our guest blogger, Aneesh... -
Plasmids 101: Control Plasmids
TypeBlog Post...decreased expression of Gene X. Ergo the positive control(s) should decrease the expression of Gene X. Since the... -
Tips for Using FRET in Your Experiments
TypeBlog Post.... 2007 Sep; 32(9): 407-14. Zimmermann T, Terjung S. FRET Basics and Applications: An EAMNET Teaching ... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog Post...interest will be expressed. The RNA polymerase(s) RNA is transcribed from DNA using an RNA polymerase... -
Negotiating Work and Life: How to Find the Joy
TypeBlog Post... not to do this. For a family with working parent(s), the sooner your kids can make a decent meal, fold... -
Troubleshooting and Optimizing a Western Blot
TypeBlog Post...trouble by confirming your transfer worked with Ponceau S staining or other reversible protein staining immediately...resources References Luo, H., Rankin, G., Straley, S., & Chen, Y. (2011). Prolonged Incubation and Stacked... -
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog Post...Foronda M, Zimmerman J, Diaz B, Zafra MP, Goswami S, Dow LE (2020) GO: a functional reporter system to... -
Plasmids 101: Plasmid Incompatibility
TypeBlog Post...2958.2005.04643.x Pubmed PMID: 15916596 Kamruzzaman M, Shoma S, Thomas CM, Partridge SR, Iredell JR (2017) Plasmid... -
Hot Plasmids Spring 2024
TypeBlog Post...many closely-packed labeled cells. Plus, Voltron2’s fluorescence is tunable by changing the dye that becomes... -
Transferable Skills Guide: Creativity
TypeBlog Post...mental-mistakes-that-kill-your-productivity Finkelstein, S. (2019, July 15). A brief exercise to spur innovation... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog Post...Many thanks to our guest blogger Gary McDowell! Gary S. McDowell is currently the Executive Director of Future... -
Site Directed Mutagenesis by PCR
TypeBlog Post...minor extension can usually ensure that the 3’-base(s) do not form secondary structures. The introduction... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog Post... tactic, and puts you ahead of most start-up P.I.’s. It doesn’t have to be complicated, or difficult to... -
27 Hot Plasmids from 2016
TypeBlog Post...PMID: 25895059 Barnea, et al. Proc Natl Acad Sci U S A. 2008. PubMed PMID: 18165312 CIDAR MoClo parts ...or clusters. Veggiani, et al. Proc Natl Acad Sci U S A. 2016. PubMed PMID: 26787909 EasyClone 2.0 Yeast...Hanson SM, Rodríguez-Laureano L, Albanese SK, Gradia S, Jeans C, Seeliger MA, Levinson NM, Chodera JD. bioRxiv... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog Post...antibiotics! References Espah Borujeni, Amin, Anirudh S. Channarasappa, and Howard M. Salis. "Translation ... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog Post...24700457. PubMed Central PMCID: PMC4077948. Yan, Kelley S., et al. "The intestinal stem cell markers Bmi1 and... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog Post...Addgene. Wang Z, Pan Q, Gendron P, Zhu W, Guo F, Cen S, Wainberg MA, Liang C. CRISPR/Cas9-Derived Mutations...