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We narrowed to 954 results for: APP

Showing: 381 - 400 of 954 results

  1. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    .... For a single action potential, GCaMP7s is approximately twice as bright, relative to baseline fluorescence...fluorescence, as the GCaMP7f variant, and is approximately 5-fold brighter than GCaMP6s. jGCaMP7f is the fastest...inactive neurons is reduced. This variant has an approximately 12-fold increase in brightness after ~100 action...genetically-defined neuronal populations with intersectional approaches is a growing field that we aim to support by ...

  2. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type
    Blog Post
    ...research. This year, we put out another call for applications. We had an overwhelming response and were excited...many creative uses of fluorescent proteins. We’re happy to announce the three winners of this year’s Michael...does that mean? Previous research shows that approximately 75% of all protein-coding pathogenic human variants...artificial leaf replica system. “This bottom-up approach will allow me to create a fully controllable and...

  3. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ...erupted in controversy. Briefly, there are two overlapping, valid critiques being offered of He’s work: ...public involvement, research, and consensus can happen. We just have to look to the United Kingdom’s Nuffield... technique was feasible, three separate things happened nearly simultaneously: The Wellcome Trust funded...biosecurity, and the entire 2018 saga of “what’s happening in genetics this time?” She lives with her partner...

  4. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ... related to PZA-resistance by using different approaches, including CRISPR and recombination-mediated ...fluorescence microscopy and FACS. In an additional approach, plasmids such as pACBSR-hyg and pFLP-hyg will... the river back to the user.   At Addgene we're happy to be able to support diverse groups of scientists...collaborations. Many thanks to all of the awardees, all who applied, and to Seeding Labs who helped us coordinate and...

  5. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...characterize a protein of interest (and we very much appreciate even those small contributions). Of course, no...incorporate FPbase data in their own projects and applications, a REST API allows programmatic retrieval of...within-study photobleaching comparisons.  We very much appreciate feedback, so try out the site and contact us ...Techniques - Which is best for me? A Practical Approach to Choosing the B(right)est Fluorescent Protein...

  6. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...in pairing with base editing and prime editing approaches. Recently, Xue Sherry Gao’s lab at Rice University...packaged into viral vectors for potential in vivo applications or multiplex CRISPR screening.   Using tRNAs...and efficient in plasmid and viral vectors. In applications when repetitive tRNA sequences must be avoided...construct DAP array for multiplex gene editing applications, we provide detailed manual and online design...

  7. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ..., or reordered and PCR enzymes are adjusted. Appropriate fragment and primer design results in covalently...transforma­­­tion or as a PCR template. Some of the applications of the NEBuilder HiFi DNA Assembly Mix include...target gene, for example, are amplified with overlapping regions of homology and inserted into a linearized...associated with otherwise more complicated assembly approaches, and opens the door for new opportunities in ...

  8. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...fluorescent signal: untranslated RNAs appear yellow, and translated RNAs appear red. To enable this color switching...and RFP are bound to the reporter, causing it to appear yellow. The act of translation pushes NLS-PCP-GFP...tethers mRNAs to the plasma membrane, it’s not appropriate for use with mRNAs usually translated in a specific...

  9. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ... with an intuitive user interface.   The team approached the issue from multiple angles: the overall user...of information so we were able to fine tune our approach much more than if we were dealing with general...” variations and synonyms of the same term that appear in our data into a single filter value. For example...our search engine to be so specialized that it appears to “understand” biology.  We hope you will give...

  10. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...engineering E. coli metabolism, maybe you’ll be able to apply your knowledge of metabolism to better understand...favorite FRET-based biosensing technique can be applied to an important neuronal signalling pathway - if...got a useful tool, there’s likely someway you can apply it in neuroscience. AAV tools from Addgene are already...targeting genes to specific cell types in the brain, mapping neuronal networks, and selectively activating signaling...

  11. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...small, avoidable mistake. Or worse, when you're trapped in a pattern of small errors that significantly...of ways you could change your variables - your approach, your workflow, or your physical setup - to prevent...workflow can have a huge impact. Test different approaches, like whether it's better for you to use cold...resistant to. Just like any other experimental approach, use small, incremental changes to understand ...

  12. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...spCas9 was the only Cas enzyme widely available and applied by researchers for gene targeting. Fast forward...properties, previously established uses, and intended applications. Within CasPEDIA, there is a classification ...multiplex ability Experimental guidelines and applications Protein structure and properties Links to ...you want to read literature on the less common applications of an enzyme for your non-traditional experiment...

  13. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ... are here to discuss the ins and outs of both approaches. Screens and selections The common goals The...lot in common. In fact, selections are often inappropriately categorized as screens! Why the confusion? ...details - and that’s where the differences in these approaches can be found. As an example, say you are considering...require there to be a culture condition that can apply enough pressure to select for variants of interest...

  14. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...review system. Peer review in traditional journals happens before the manuscript is accepted for publication...at the same time. Take your time picking the appropriate journal and explain in your cover letter why ...why you think your manuscript is appropriate for that journal. Argue with an editor or reviewer over their...ignored their comments, they will not be inclined to approve your paper. Rush your submission or revision. A...

  15. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...with the same genetic background and do an apples-to-apples comparison of all those mutations. If you ...take four plasmids out of the library in the appropriate combination and do a restriction enzyme digest...different genetic backgrounds. It becomes more of an apples-to-oranges comparison. Addgene: Can you compare...

  16. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    ...systematically mutated it. Using a robotic picking approach to select cells with variant characteristics they...” vector cloning system - a Gateway compatible approach to simultaneously clone two genes-of-interest ...response to blue light (445nm). The discovery and applications of protein partners that bind to only the light...the photoswitchable proteins offers a powerful approach to control protein-protein interactions in cells...

  17. Summer SciComm: Preprints

    Type
    Blog Post
    ...poster or talk, or submitting patent or grant applications; to informal settings like lab meetings, social...listing “Submitted” articles in their CV or grant applications. A number of organizations (such as ASAPbio)...and preprints are a great way to do that. We are happy to receive deposits of plasmids that have been described...asapbio.org/preprints-and-covid-19 Ettinger CL, Sadanandappa MK, Görgülü K, Coghlan KL, Hallenbeck KK, Puebla...

  18. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    ... Haoyi Qiu He Tian Every two years, we seek applications for awards to early career scientists using ...virtual conference attendance. We also opened up applications to research technicians and undergraduate researchers...researchers to better understand the processes that happen inside the cell,” says Pedre Pérez. Figure...construction of the dual virus and the experimental approach. (B) CR-Cre mice demonstrate pain response due...

  19. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...previous post in this series described a practical approach to selecting a bright fluorescent protein. In ...reached at high power may not translate to real applications in which far less power is used. For example...insight into the photostability in the intended application (Goedhart et al, 2012). The third issue relates...proteins. To avoid diffusion, the proteins are (i) trapped in microdroplets in a water/oil emulsion, (ii) ...

  20. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...several methodologies currently available for approaching it. These include the traditional restriction...introduction.html demonstrating the principal behind the “swapping out” of ccdB with the investigator’s gene of interest...which can then be used for downstream cloning applications. While these two strains ultimately perform ...important to ensure that you choose the most appropriate method for your own experiments. Please check...
Showing: 381 - 400 of 954 results