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We narrowed to 970 results for: Abo;

Showing: 381 - 400 of 970 results

  1. Plasmids 101: Repressible Promoters

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    Blog Post
    ...can be used in the repressible manner described above, as well as the inducible manner detailed in our...necessary to have all 3 of the different binary systems above if they all behave very similarly?” The simple answer... at the same time.  The 3 binary systems listed above are orthogonal, meaning that they do not affect ...Srikrishnan. “Introduction and expression of genes for metabolic engineering applications in Saccharomyces cerevisiae...

  2. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...Blog Learn more about FRET Tips for Using FRET in Your Experiments Learn more about Fluorescent Protein...heme-containing proteins in whole animals, light at or above 600 nm wavelengths should be used. Researchers often... emits light at a tissue-penetrating wavelength above 600 nm. LumiFluors Schaub, F.X., et al. (3) designed...be excited by NanoLuc® luciferase (NLuc) and set about to create an in vivo imaging reporter by linking...

  3. 5 Science Rap Videos & Their Creator: Tom McFadden

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    Blog Post
    ...Science with Tom. In this podcast, we learn more about Tom and pick his brain for advice on how to dive... Rap of Henrietta Lacks Students in Oakland rap about Henrietta Lacks - the African American woman whose...Please Don't Kill My Hive Beautifully filmed video about colony collapse disorder.     5. Plasmid Rap ...science standards and communicate science. Learn more about Tom on his youtube channel and at sciencewithtom.com...

  4. Tips for Working in Industry & Success in Management: Interview with Crystal Shih

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    Blog Post
    ...therapeutics at Novartis - listen to learn more about Crystal’s exciting career path, to gain insight ...Chapter 2: What's a lab group in industry? The collaborative nature of working in industry and how delegating...metrics for success. The importance of thinking about the big picture and not just the next experiment...resources in our Mentoring for Scientists Series Learn about Turning Your PhD into a Career with Joanne Kamens...

  5. New Podcast Segment: Hot Plasmids

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    Blog Post
    ...this new way to learn about plasmid technologies. If you have any comments about the Hot Plasmids Podcast...with these efforts, you’ll find new ways to learn about science careers, lab protocols, and, of course, ...each one, plug in some headphones to learn more about the latest and greatest plasmids through this podcast...

  6. Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service

    Type
    Blog Post
    ...Plasmids 101 blog series Learn about the different grades of DNA  Read about the various ways to quantify...scientific communities using plasmid tools. Details about the cloning grade DNA beta project We will ship ...quantify DNA Resources on Addgene.org Learn about our other beta projects Read our Molecular Biology Reference...

  7. A History of Genome Engineering in Popular Culture

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    Blog Post
    ...Find more blog posts about CRISPR Learn more about the history of CRISPR Read about other genome editing...Science Fiction (SciFi) writers have been curious about the capability of transforming the genetic code ... of California San Diego. She is working in collaboration with Prof. Alexis Komor and together they are...

  8. Important Considerations When Using AAVs

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    Blog Post
    ... Its Fruit Is Sweet The Future? I knew little about adeno-associated Viral Vectors (AAVs) before starting...I’ll provide some tips that will help you think about how to get started with AAVs. Since my research ...understanding of receptor physiology and the effects of metabotropic and ionotropic influences on overall cell function... the biotechnology firm Biogen have recently collaborated to develop “AAV 3.0”, vectors that will have...serotonin in movement. Follow her on twitter @katrinaboards.   References 1. Russell, David W. "AAV vectors...

  9. Bringing Sustainable Practices to the Lab: Easy Action Items

    Type
    Blog Post
    ...blog Learn about recycling initiatives for the lab Subscribe to the Addgene blog Learn about other creative.... In addition to doing some of the quick things above, try joining or starting a sustainability project...campaign! MIT Green Labs has a lot of resources about this, and even has a list of biological samples ...

  10. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ...How did you hear about post grad opportunities and when did you start thinking about them? 18:30 - 19:... top if you leverage your community, think hard about picking a good mentor, and begin considering career...19:54 : How to think about picking a post-doc project 19:55 - 22:40 : Improvements that can be made to ...

  11. Fueled by Coffee at #SfN14

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    Blog Post
    ...learn about all the amazing research being done with plasmids. I heard an impressive talk about the use...pulls off such an inspiring, thought-provoking, collaborative event – all while keeping the party going! At...scientists we've spoken with are excited and passionate about making such tools open-access. Though it is time...

  12. 2022 and 2023: Reflections and Looking Forward

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    Blog Post
    ...updated AAV Data Hub to share curated information about AAV usage in research applications. Scientists around...world, are learning to navigate the changes brought about by the pandemic. As we adjust, we are reflecting...I have spent the last 9 months quietly learning about Addgene’s past and present, but the real promise...forward to working with Addgene colleagues, collaborators, partners, and the larger scientific community...

  13. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    ... questions about the ordering process, the Addgene Help Center is full of information about how to deposit...requires a quote in order to generate a PO, learn more about how to create a quote. How do I pay the invoice...Addgene Help Center If you have additional questions about our ordering process not addressed in this article...

  14. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ... highlighted reagent repositories and other collaborative efforts in the scientific community to speed...store my plasmid in TE, Tris, or water? And what about temperature? Find the details in this blog post....dominates DNA quantification discussions, but what about the other methods to quantify DNA? Here we cover...Incompatibility by Leah Schiesow If you're thinking about hosting multiple plasmids in one cell, it might ...

  15. Plasmids 101: Modular Cloning Applications and Kits

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    Blog Post
    ... constructs that compose genetic circuits and metabolic pathways. For many MoClo systems, the individual...interested in recombinant protein purification and metabolic engineering. If instead you want to build genetic...designed for flexibility. The Fragmid kit described above works in both mammalian systems and Drosophila. ... Signaling in CAR T Cells. Cold Spring Harbor Laboratory, 17 Feb. 2024. Crossref, https://doi.org/10.1101...Emerging CRISPR Technologies. Cold Spring Harbor Laboratory, 27 Oct. 2023. Crossref, https://doi.org/10.1016...

  16. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...environment? If these are pressing issues in your laboratory, then FRET experiments are a viable biophysical...to consider these cases separately when talking about the tools that are available. Let’s consider the...that, if you make an FP fusion, you are inserting about 20-25 kDa of protein mass into your protein and ...together The FRET donors and acceptors described above can be paired together to measure a wide range of...of your protein that can lead to novel insights about its biology and its behavior. See the table below...

  17. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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    Blog Post
    ...ChIP-seq methods provide a wealth of information about how protein networks work in cells. A major bottleneck...two-part cr/tracrRNAs for CRISPR experiments (in collaboration with Integrated DNA Technologies, USA). These...commercial suppliers without investing time and labour on reagent preparation in the lab. Figure 3: csRNP-assisted...first crRNA we designed for ~250 genes, we have had about 30% tagging success, although many of these genes... When not pipetting, he can be seen daydreaming about sunny days in the UK.   References 1. Bressan RB...

  18. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ... to design gRNAs for all the purposes discussed above. Please see videos and text on the website for more...Output: A text file containing full information about the suggested gRNAs to use, including all information... hard to read. It´s suggested that you open the above text files, copy the content of the file, and paste...Additional Resources on the Addgene Blog Learn about CRISPR Screens How to Design Your gRNA Tips for ...CRISPR User Resources on Addgene.org Learn More About CRISPR Find CRISPR Screening Libraries Browse All...

  19. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    ...post, a study coming out of MITs Human Dynamics Laboratory identified five characteristics of very successful...conversations within the team. We are not talking about gossip here – this means encouraging a culture of...Medical School researcher Connie Cepko to learn about her management style.   Seeking feedback frequently...information to make change whenever possible. Be public about changes influenced by feedback. If you don't do ...

  20. Inside Addgene's 10 Year Anniversary Gala

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    Blog Post
    ...Joanne Kamens, Melina Fan, and Ken Fan each spoke about what Addgene means to them. Ken mentioned Addgene...Addgene could not do what it does.  Melina talked about her grandfather’s humble beginnings as an operator...and add to this post. Interested in learning more about the last 10 years @Addgene Check out our impressive...stats from 10 Years of Plasmid Sharing Or read more about what happens Inside Addgene   ...
Showing: 381 - 400 of 970 results