... John Doench et al. shows that mutating a single base pair may not be enough to prevent re-binding. Lastly...region in a UTR, CrispyCrunch flips the two important base pairs of the PAM (NGG -> NCC, CCN -> GGN). This ... much as possible, it uses standard tools and databases, including primer3, biopython, bwa, samtools, ...

... electroporations. The specifics of this can be based on the manufacturer’s protocol for transformation...transformation efficiency, or more specifically calculated based on the size of and weight of your library plasmids...

... different categories based on function: knockout (KO), activate, inhibit, base edit, and other. The first...

...but their initial results show promise for CRISPR-based gene activation. dCas9-CBP In this method, dCas9...2013) RNA-guided gene activation by CRISPR-Cas9–based transcription factors. Nat Methods 10:973–976 . ...

...point where the hydrogen bonds break between the base pairs. This results in the separation of the two...using PCR. qPCR requires the addition of a probe based fluorescent dye that intercalates with any dsDNA...

... scanning By Seth Kasowitz As multiple antibody-based vaccines are deployed around the world to combat...deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage tracing tool captures rare clones that represent...

...available filtering options now change dynamically based on the type of material and specific search terms...curated pages categorized by both functionally (e.g. Base Editing) and by target organism. Clicking through...

... question to ask is how the efficiency of PITCh-based genomic insertion would compare to CRISPR sticky-end...Mathieu, et al. (2015). “A Scalable Genome-Editing Based Approach for Mapping Multiprotein Complexes in Human...

...and fluorescence. Biosensors: A wide array of GFP-based fluorescent biosensors has been designed to detect...separates mixtures of cells into distinct populations based on fluorescent signal. Thus, FACS can be used to...

...antibodies in Addgene’s repository  IBEX Knowledge-Base  Any IBEX multiplex tissue imaging Data repository...

...Novartis, a multinational pharmaceutical company based in Switzerland. After completing her PhD in Chemistry...

...consistent development and improvement of CRISPR-based technologies over the past decade. CRISPR screens... fact supported by the wide array of lentiviral-based CRISPR libraries available at Addgene. The large...

...less photostable than GFP. In addition, antibody-based amplification of their signals is weak and increases...specific antibody binding sites and primary antibody-based detection, smFPs create much brighter signals for...

...different ideas. We are a huge fan of the Golden Gate based MoClo system. The vectors mentioned in this blog..."The FAST technique: a simplified Agrobacterium-based transformation method for transient gene expression...

...money. They also usually have a good event-planning base and will know how to reserve space, time events,...I think many of the students learned something. Based on the initial guidelines I received from the teachers...

...allowing for in vivo biotinylation through a plasmid-based biotin ligase. Biotinylated dCas9, with bound DNA...genomic region is another example of the many tools based on targeting of a genomic locus through a specific...

...arises here. Notwithstanding access restrictions based on permissions, however, in some cases it would ...provides the RSpace electronic lab notebook. Rory is based in Cambridge, Massachusetts, and feels privileged...

...additionally allow for visual selection of transformants based on expression of RFP in the seed (Emami, Yee and...inflorescence (the flowering portion of the plant) near the base of the stem. This buys you a few days until new ...

...extrapolate the concentration of the unknown sample based on its measured response. An example of this is ..., the more accurate the curve. Though it varies based on the assay, the ideal %CV is less than 15%. You...

...Addgene blog Introduction to Antibodies Plasmid-based Recombinant Antibodies Polyclonal Antibodies Resources... Shokri A, Chotteau V. Antibody capture process based on magnetic beads from very high cell density suspension...