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We narrowed to 644 results for: CAR

Showing: 381 - 400 of 644 results

  1. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...problem-solving process?  You may want to start by carving out time to tackle it. It’s easy to get distracted...been building your creativity all along. In my own career, my team has used these techniques to optimize ...

  2. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...Overview An immunoprecipitation reaction is typically carried out in one of two ways. Both methods utilize the...approach appealing, but the disadvantages must be carefully considered before using. For example, the tag ...

  3. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ... in cold conditions and preferably on dry ice. Careful use of your glycerol stocks When your lab adopts...experiments. The term ‘insert’ refers to plasmids carrying each part, ‘vector’ refers to acceptor plasmids...

  4. Viral Production at Addgene

    Type
    Blog Post
    ...line is maintained for ~20 passages before being discarded and replaced with a new vial of early passage ...produced in the line will be taken offline and discarded. Confirmation of the transfer plasmid Addgene ...

  5. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...chromosomal DNA. Chromosomal replication in bacteria is carefully regulated to ensure that it occurs at the appropriate...DNA replication in bacteria, so the process is carefully regulated by a mix of positive and negative regulators...

  8. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...antibiotics is certainly a topic of great concern in healthcare today, but it doesn’t have to be some looming...resistance from the CDC. Antibiotic resistance can be scary to think about, but we can all do our part to learn...

  9. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...promoter on the plasmid. Many commercial strains carry the DE3 lysogen, as indicated by the name of the...reasonable time-frame. For these cases, some strains carry an additional measure of control such as the pLys...

  12. March for Science

    Type
    Blog Post
    ...issues Reproductive health Immigration Pollution Healthcare Science education To make scientists relatable...Scientists' Views PolitiFact: EPA head Scott Pruitt says carbon dioxide is not 'primary contributor' to global ...

  13. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...Equipment needed: Mini PCR machines by DIY-Bio, P51 cardboard fluorescence visualizer with 9V battery Approach...fluorescently labelled RNA, the authors employ a P51 cardboard fluorescence visualizer that is powered by a 9V...

  14. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...coupled with your technical skills, can open new career opportunities for you, if you can identify them...eye out for the phrase “I assumed they were taking care of that”, because that can indicate confusion around...

  15. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...available resources and give yourself time to carefully review your options. If possible, try to select...fluorescence-microscopy/   Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, ...

  17. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...First, CRISPR would need to be delivered to both cardiac and skeletal muscle cells, where precision editing... each observed the same positive phenotypes in cardiac and skeletal muscle. Even when the genome editing...
Showing: 381 - 400 of 644 results