We narrowed to 649 results for: CHI
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TypeBlog Post...Determine what type of outcome you are trying to achieve: Do you want to permanently knock out or knock-... All of these outcomes can be most effectively achieved with different CRISPR components. Select the appropriate...
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Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog Post...with Golden Gate MoClo. The barrier for those switching to Golden Gate is a conceptual barrier. Sometimes... of the university impacts the number of women reaching senior positions. There has been a lot of engagement... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog Post... Chemistry at Connecticut College who has been teaching and communicating science for over two decades...as pertinent now as it was over 300 years ago. Teaching by example No examination of the state of science... -
Summer SciComm Series: Modes of Communication
TypeBlog Post...scientific training. Cartoons may be read as “for children” or may need text for audience interpretation....experience. Can combines cons of multiple medias. Switching from one media type to another is cognitively ... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog Post...using a plasmid such as pET-28b-Cas9-His from Alex Schier’s Lab. gRNAs can be in vitro transcribed from ssDNA...publicly available by both the Jacob Corn and Alex Schier laboratories. Delivering Cas9-gRNA ribonucleoproteins... -
Getting to Know Expansion Microscopy
TypeBlog Post...limited in the level of resolution that they can achieve. This means that regardless of how powerful of ...evenly in all directions, aka isotropically. But achieving isotropic expansion depends on how well the gel... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog Post...opportunities for his students. But he also found that his teaching responsibilities left him with limited time for...the U.S. Army, and hockey coach all shaped his teaching style, with the former offering excellent training... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog Post...RNA that bind to a cell’s DNA, telling the Cas machinery where to cut. Fig. 2: Heatmap indicating...when interpreting findings, particularly when researching ancestry groups. And, of course, it’s imperative... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog Post...fluorophore can emit a limited number of photons before bleaching. The amount of information one can extract from...cell biologist can feel like a college student searching couch cushions for spare change, desperate to ... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog Post...gene fusions for analyzing gene expression in Escherichia coli and yeast. Methods Enzymol. 1983;100:293...2547163. 5. Ozawa, T., Kaihara, A., Sato, M., Tachihara, K., Umezawa, Y.l. Split luciferase as an optical... -
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments
TypeBlog Post...last month it’s been viewed by users in the US, China, India, Canada, the UK, South Korea, and beyond!...protocols So that’s great, but what if someone in China improved upon that protocol? How would the thousands... -
Negotiating Work and Life: How to Find the Joy
TypeBlog Post... yourself, “I’m not married,” or “I don’t have children, so my life will be under control.” Think again...starve while you are away a few days. Also, your children will enjoy day care and school if you tell them... -
Rett Syndrome: A History of Research and Therapeutic Outlooks
TypeBlog Post...hope that a similar reversal may be possible for children and adults with Rett Syndrome. Since these discoveries...these approaches, such as delivery of the editing machinery to the brain, minimizing off-target editing, and... -
Finding nucleic acids with SHERLOCK and DETECTR
TypeBlog Post...10.1126/science.aaf5573 Broughton JP, Deng X, Yu G, Fasching CL, Servellita V, Singh J, Miao X, Streithorst...Miller S, Pan C-Y, Guevara H, Wadford DA, Chen JS, Chiu CY (2020) CRISPR–Cas12-based detection of SARS-CoV... -
Save Time with Transient Plant Leaf Transformations
TypeBlog Post... a virus and the plant's post-transcriptional machinery to silence genes of interest (Unver and Budak,...plants." Plant Biology 5.02 (2003): 103-115. Hu, Ching-yeh, et al. "Intrinsic GUS-like activities in seed... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog Post...Strongylocentrotus purpuratus, the spiny purple sea urchin. It’s not a standard model organism, but it was...neurobiology, and more. Prochlorococcus (Penny Chisholm) This cyanobacterium is the smallest photosynthetic... -
CRISPR-mediated Plant Base Editors
TypeBlog Post...efficient base rewriting in the genome became achievable in 2016 when two independent groups (Komor et...is either repaired by cellular mismatch repair machinery making an original C-G pair converted to T-A or... -
Neuronal labeling with Spaghetti Monster
TypeBlog Post...streptavidin-binding strep-tag and, more recently, OLLAS (Escherichia coli OmpF linker and mouse langerin) and Sun ...Spaghetti Monster is a symbol of opposition to the teaching of intelligent design in public schools and was... -
How to Write a Scientific Review Article
TypeBlog Post...particular topic. Alternatively, maybe you’re researching a topic for which no relevant or recent review...Blog Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing Tips for Improving Your... -
Using Video to Share Your Science: We Share Science
TypeBlog Post...Videos Over at the Addgene Youtube Channel After watching these trends for a few years, I decided to look... brief introduction to the research and, after watching the video to determine if you’d like to learn ...