... for your cell lines and/or tissue types.  Please do note my oh-so-subtle use of reference genes, plural...reference genes - and it is well worth your time to do one (Biassoni, 2014). At the bench As a highly sensitive...you from understanding how the analyses work - you do need to make sure the assumptions in your dataset...end of your analysis process. You’ll still need to do statistical analysis to determine if there are any...

...many scientific questions. But these tiny creatures do tend to come with a rather astonishing ability to...

...Cancer. 2003 Jan;3(1):11-22. PubMed PMID: 12509763 . Do you have suggestions for other plasmids that should...

...specific repetitive DNA sequences in human chromosomes Do you have suggestions for other plasmids that should...

...more versions of this plasmid. Root Return to Top Do you have suggestions for other plasmids that should...

...transfer plasmids do not have selectable markers conferring resistance to an antibiotic, but do encode another...pRRE and pRSV-Rev . 3rd generation packaging systems DO NOT express tat. Third generation lentiviral systems...Addgene's Plasmid Reference and Protocol Guide . How do I clone my insert into a transfer vector with no ...system in total). In addition, 3rd generation systems do not use the HIV protein tat in order to produce full...

...fluorescent proteins or localized fusion proteins and do not require dedicated plasmids or constructs. It ...allow for a well-controlled environment, but they do not mimic the natural situation. Measuring photostability...

...ability to target almost every genomic locus, and to do so in vivo. Catalytically dead Cas9 (dCas9) cannot...CRISPRainbow requires very short, 11-mer gRNAs, which do not induce genome editing. If used with catalytically...

...inducer. Since induction via estradiol and progesterone do not affect each other, these systems can be used ...random variation, or noise, in reporter expression. To do so, one places reporter expression under the control... et al. Cell. 2017. PubMed PMID: 29153837. Endosomal pH sensors for STED nanoscopy Article contributed...contributed by Eric J. Perkins Listen to Endosomal pH Sensors podcast segment The first Addgene plasmid deposit... biosensors for super-resolution microscopy.” Endosomes are historically difficult to study in living ...met some of the criteria necessary for studying endosomes, the Hell lab designed their super-resolution ... meet all of them. These probes had to target endosomes, be exceptionally bright (which is necessary for...

...different epitopes on the target protein, so they do not compete with each other. This antibody is also...interest that is present. Using a Standard Curve To do that calculation, you’ll need to include a standard...

...had an industry interview before. What should you do to get ready for this interview? Maybe the above ...the same boat, but there are a few things you can do to make the experience less stressful. First, you...

...   Viral vectors So, what do viral vectors have to do with all of these cool tools? Calcium...

...virus to mice, part of your preparation should be doing your best to be well-rested and alert the day of...procedure. Mice move much more quickly than cell cultures do, and tired researchers are accident-prone researchers...

... cell and animal models. AAVs are non-pathogenic, do not integrate into the host genome, and can be specifically...several years (Wonjo et al., 2013). Remember that AAVs do not integrate into the host genome and therefore ...

...Imagine an antibody. Do you immediately visualize a Y-shaped protein reminiscent of the Addgene mascot...sure to think about what pieces of the antibody you do and don’t need, and how the protein will interact...

...during your western and your results look… funky? Do you need to start a whole new blot? Never fear: membrane...directly to buffer without a re-wetting step. Important: do not dry your membrane with antibodies on it if you...

... only problematic word. How many different things do you simply refer to as a “tube”? We took steps towards...lab or collaborators will not have the context you do, which means you’ll need to provide that context ...

... all have their benefits and drawbacks. Labs that do not routinely produce virus may want to choose more...Additional Resources on Addgene.org Learn How We Do Viral Production at Addgene Read Our Lentiviral Guide...

...fragments. When designed correctly, the recognition sites do not appear in the final construct, allowing for precise...these sites are eliminated by digestion/ligation and do not appear in the final construct. The destination...

...their current readership. Encouraging students to do science anywhere.  Chapter 4 - JEI's mission and ...