We narrowed to 821 results for: EED

...note, there is no minimum number of markers that needs to be used with spectral flow—you can run a three...co-expression among cellular markers You’ll also need to know if markers are co-expressed in your cells...have a level of background autofluorescence that needs to be accounted for in order to distinguish the ...

...planning, you’ll know exactly what materials you’ll need to bring into the hood and can wipe them all down...cellular metabolism that is toxic to the cells), and need to be split very soon. There is power in numbers...or contaminated. This also ensures that anyone needing new cells in the lab can take a vial of the ‘mint...

...genome with fluorescent proteins. For each gene, we needed to design guide RNAs, donor templates and sequencing... such a scale, automation is crucial, both for speeding things up and for standardizing the process. When...mutates HDR donor templates to avoid re-cutting when needed. It gives you control over your data. CrispyCrunch...

...marker proteins, however, in this case the cell needs to be fixed and the cell membrane permeabilized ...problems associated with fluorescent imaging such as bleed-through (the incorrect detection of one fluorescent...baseline rule, the emission spectra of the selected FPs need to be sufficiently separated, most commonly FPs ...

...assembly levels were indeed possible, with correct, in-frame assembly proceeding in 99% of 12-fragment...assembly that would result in a blue phenotype. Indeed, this was seen in all lacI/ lacZ assembly test ...

...binding domains forming a building block sufficient to seed polymer formation as fusion protein concentration...designated mEGFP, mVenus, etc. A word of caution is needed because FPs not derived from GFP, i.e. the red ... FPs Find the Best Fluorescent Protein for Your Needs Get Tips for Using FRET in Your Experiments Additional...

... Cloning easier because individual labs will not need to ‘domesticate’ all the basic and necessary elements...s really a matter of choosing the parts that you need from the kit and getting started making your own...They just start and go ahead. I can see science speeding up. Addgene: Are there other tools you are working...

...race, it highlights the myriad issues researchers need to reflect upon before they attempt to make essentialist...of a true correlation.   It turned out there was indeed a bias, and it was coming through an unexpected...screens, they had also found the information they needed to begin correcting the issue. Working with James...

...vector performance. Yet, most of the technical data needed to replicate and build on others’ results is available... is a free resource to share this data and help speed scientific progress, but it will only be effective...Open Data Day with Addgene’s AAV Data Hub!Data Freedom: The Expansion of Data Sharing in Research Publications...

...have the annoying tendency to runout right when you need them and to accumulate a bunch of unused buffers... can still reduce the number of spin columns you need to complete your research. Do you know of other ...analytical purification of DNA from agarose. Proceedings of the National Academy of Sciences of the United...

...terminals, which are jam packed with everything needed for synaptic transmission, are right around 250...Microscopy (EM) is one option, but the equipment needed for EM is not something you find in the average... don’t have access to the specialized equipment needed for EM or SRM? Enter Expansion Microscopy. What...

...purify, and analyze MDH in semester one and then proceed to use those skills to design and carry out their...genuine interest to other researchers in their field. Indeed, sharing results with the broader scientific community...Mentorship and protégé success in STEM fields. Proceedings of the National Academy of Sciences, U.S.A. 117...

...does not drop significantly until protein levels exceed 70% of the sample content (Loughrey and Matlock...2019). To confirm RNA in your DNA sample, you will need to use a complementary assay, such as a fluorescence...readings are inherently more variable. If you do need to work with dilute samples:  Take multiple readings...

...are planning, cost, and the amount of protein you need to produce.  The most common yeast strain used for...processing. It may be helpful to incorporate regular feeds during this incubation time to maintain cell viability...absorbance to 280 nm (Pace et al., 1995). You’ll also need to know the extinction coefficient to calculate ...

...of a donor DNA molecule. Your donor molecule will need to have homology to the targeted locus on either...your donor molecule is used as the template, you need to incorporate homologous sequence to the right ...larger knock-in (200 bp – 2 kb), a dsDNA donor will need to be used due to synthesis limits of oligonucleotides...

...likely won’t solve the growing problem. Above all, we need new antimicrobials, ideally ones for which the development...resistance will be slow. CRISPR may be just the method needed to jumpstart the fight against antibiotic resistance...Thus, a very large number of phagemids would be needed for an in vivo therapy. Another strategy for CRISPR...

...young researchers need to publish good publications in a short period of time and may need to do some fashionable...mechanisms of regulation of genome functions, we need to identify molecules associated with specific genomic...

...lab and offering advice on areas where you might need some help. Today, we’re highlighting an oft-underlooked...if it were a meeting. Gather the resources you’ll need ahead of time and mute your notifications during...At some point or another, you’ll find yourself needing to brainstorm with a team. One major challenge ...

... et al., 2018). To circumvent sequencing and the need for a DSB in their previous reporter, the Harris...eGFP reporters from the Harris Lab circumvents the need for DSBs in monitoring on-target DNA editing efficiency...) to purify single base edited cells without the need for single cell isolation and downstream sequencing...

...still take a community to advance our knowledge. We need to continue to develop improved, reliable tools ...Omstead, V., Vargas, S., Lerma, M. N., Taskin, N., Weed, N., Laird, W. D., Bishaw, Y. M., Bendrick, J. L... A., Kalmbach, B. E., Radaelli, C., Gore, B. B., Weed, N., Omstead, V., Bishaw, Y., Shapovalova, N. V....