Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 825 results for: EED

Showing: 381 - 400 of 825 results

  2. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    ... Cloning easier because individual labs will not need to ‘domesticate’ all the basic and necessary elements...s really a matter of choosing the parts that you need from the kit and getting started making your own...They just start and go ahead. I can see science speeding up. Addgene: Are there other tools you are working...

  3. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...vector performance. Yet, most of the technical data needed to replicate and build on others’ results is available... is a free resource to share this data and help speed scientific progress, but it will only be effective...Open Data Day with Addgene’s AAV Data Hub!Data Freedom: The Expansion of Data Sharing in Research Publications...

  4. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...terminals, which are jam packed with everything needed for synaptic transmission, are right around 250...Microscopy (EM) is one option, but the equipment needed for EM is not something you find in the average... don’t have access to the specialized equipment needed for EM or SRM? Enter Expansion Microscopy. What...

  5. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    ...does not drop significantly until protein levels exceed 70% of the sample content (Loughrey and Matlock...2019). To confirm RNA in your DNA sample, you will need to use a complementary assay, such as a fluorescence...readings are inherently more variable. If you do need to work with dilute samples:  Take multiple readings...

  6. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...are planning, cost, and the amount of protein you need to produce.  The most common yeast strain used for...processing. It may be helpful to incorporate regular feeds during this incubation time to maintain cell viability...absorbance to 280 nm (Pace et al., 1995). You’ll also need to know the extinction coefficient to calculate ...

  7. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...purify, and analyze MDH in semester one and then proceed to use those skills to design and carry out their...genuine interest to other researchers in their field. Indeed, sharing results with the broader scientific community...Mentorship and protégé success in STEM fields. Proceedings of the National Academy of Sciences, U.S.A. 117...

  8. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...of a donor DNA molecule. Your donor molecule will need to have homology to the targeted locus on either...your donor molecule is used as the template, you need to incorporate homologous sequence to the right ...larger knock-in (200 bp – 2 kb), a dsDNA donor will need to be used due to synthesis limits of oligonucleotides...

  9. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...race, it highlights the myriad issues researchers need to reflect upon before they attempt to make essentialist...of a true correlation.   It turned out there was indeed a bias, and it was coming through an unexpected...screens, they had also found the information they needed to begin correcting the issue. Working with James...

  10. DNA Purification Without a Kit

    Type
    Blog Post
    ...have the annoying tendency to runout right when you need them and to accumulate a bunch of unused buffers... can still reduce the number of spin columns you need to complete your research. Do you know of other ...analytical purification of DNA from agarose. Proceedings of the National Academy of Sciences of the United...

  11. CRISPR Antimicrobials

    Type
    Blog Post
    ...likely won’t solve the growing problem. Above all, we need new antimicrobials, ideally ones for which the development...resistance will be slow. CRISPR may be just the method needed to jumpstart the fight against antibiotic resistance...Thus, a very large number of phagemids would be needed for an in vivo therapy. Another strategy for CRISPR...

  12. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    ...young researchers need to publish good publications in a short period of time and may need to do some fashionable...mechanisms of regulation of genome functions, we need to identify molecules associated with specific genomic...

  13. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ... and by offering advice on areas where you might need some help. Today in our transferable skills guide...going alone - if you are aware of a problem or a need for something, usually someone else has realized...these types of events can be very tough. First I needed to find the right contacts at the companies and...

  14. Lab to Office Culture Shock

    Type
    Blog Post
    ...very likely be on a team that supports you when you need it. The opportunity to be creative and think critically...actually spending doing work. Yes, you may legitimately need to be in the lab that long because of the nature...during the day was also a scientist. But now you also need to talk to Tony from legal, Diana in finance, and...

  15. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...Just as researchers need to access and reference external data sources, they also need to continue to make... at the characteristics and capabilities an ELN needs to facilitate true reproducibility. The following...

  16. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ... et al., 2018). To circumvent sequencing and the need for a DSB in their previous reporter, the Harris...eGFP reporters from the Harris Lab circumvents the need for DSBs in monitoring on-target DNA editing efficiency...) to purify single base edited cells without the need for single cell isolation and downstream sequencing...

  17. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...lab and offering advice on areas where you might need some help. Today, we’re highlighting an oft-underlooked...if it were a meeting. Gather the resources you’ll need ahead of time and mute your notifications during...At some point or another, you’ll find yourself needing to brainstorm with a team. One major challenge ...

  18. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ...developing the MoClo system, researchers wisely agreed on universally using the same four-base overhangs... four-base overhangs flanking each part, as they need to match sequentially in order to properly ligate... high number of assemblies, you will also likely need to miniprep a lot of plasmids from your bacterial...

  19. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...for a bacterial infection. While this post will, indeed, focus on bacteria, it is important to note that...concentration, or sometimes more exposure time is needed for the drug to work… if the instructions for treatment...or not, because scientists in the laboratory will need to isolate and grow whatever bacteria are causing...

  20. The Challenges of Cell Culture

    Type
    Blog Post
    ...line maintenance The key is good recordkeeping, you need to have a way of tracking how many passages the ...reliable. When you order a new cell line you will need to grow this initial stock and create a set of vials...The future Attitudes are changing but yet more is needed to convince people of some of the basic tenets ...
Showing: 381 - 400 of 825 results