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We narrowed to 829 results for: EED

Showing: 381 - 400 of 829 results

  1. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...number, media volume, and plasmid concentrations will need to be optimized prior to starting. We recommend ... experimental volumes accordingly. While you may need to make some final adjustments, this is generally... to start. If you don’t anticipate that you will need large volumes of lentivirus routinely, you may opt...

  2. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ... such as non-human primates and humans. Since we need larger-volume antibodies for thicker tissues and... Exposure time is the amount of time the sample needs to be under light to discern the signal. If the ... 27–29 (2015). https://doi.org/10.1038/518027a Freedman LP, Cockburn IM, Simcoe TS (2015) The Economics...

  3. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...mechanisms and, eventually, develop treatments, we need tools to specifically edit methylation sites.  Step... DNMT1 even through replication. The dCas9 also needs to be removeable, otherwise it will continue to ...nuclear environment. One good system to balance these needs is the lentiviral-based expression of floxed dCas9...

  4. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...underpinnings of neurological disease, neuroscientists need to be able to probe cells and circuits without disrupting...chemogenetic system To set up a chemogenetic system, you’ll need to choose a method for targeting receptor expression...cell types, or even subcellular regions of a neuron need to be targeted. Targeting is also important in the...

  6. Tips for Technical Support Calls

    Type
    Blog Post
    ... And finally, provide feedback. If you are unsatisfied with the nature or speed of a response, let your...know (nicely, please!). We can’t improve without feedback. And if you had a very positive experience, also...

  7. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...switch can be created for your specifc experimental needs. How does the FLEx switch work? Let’s say you want... switch to successfully work, the cassette would need to contain the BFP coding sequence in the sense ...SSR of choice is introduced, recombination can proceed either by first utilizing the loxP sites or the...

  9. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...planning, you’ll know exactly what materials you’ll need to bring into the hood and can wipe them all down...cellular metabolism that is toxic to the cells), and need to be split very soon. There is power in numbers...or contaminated. This also ensures that anyone needing new cells in the lab can take a vial of the ‘mint...

  10. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    ...genome with fluorescent proteins. For each gene, we needed to design guide RNAs, donor templates and sequencing... such a scale, automation is crucial, both for speeding things up and for standardizing the process. When...mutates HDR donor templates to avoid re-cutting when needed. It gives you control over your data. CrispyCrunch...

  11. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...signal input to readable output, which generally exceeds that of the VSD GEVIs. However, since rhodopsin... specific targeting of these voltage indicators needs to be cell type specific as well as specifically...10.1186/s12915-019-0682-0. Beck, C., Gong, Y. A high-speed, bright, red fluorescent voltage sensor to detect...

  12. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...note, there is no minimum number of markers that needs to be used with spectral flow—you can run a three...co-expression among cellular markers You’ll also need to know if markers are co-expressed in your cells...have a level of background autofluorescence that needs to be accounted for in order to distinguish the ...

  13. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    ...marker proteins, however, in this case the cell needs to be fixed and the cell membrane permeabilized ...problems associated with fluorescent imaging such as bleed-through (the incorrect detection of one fluorescent...baseline rule, the emission spectra of the selected FPs need to be sufficiently separated, most commonly FPs ...

  15. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    ... Cloning easier because individual labs will not need to ‘domesticate’ all the basic and necessary elements...s really a matter of choosing the parts that you need from the kit and getting started making your own...They just start and go ahead. I can see science speeding up. Addgene: Are there other tools you are working...

  16. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...are planning, cost, and the amount of protein you need to produce.  The most common yeast strain used for...processing. It may be helpful to incorporate regular feeds during this incubation time to maintain cell viability...absorbance to 280 nm (Pace et al., 1995). You’ll also need to know the extinction coefficient to calculate ...

  17. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...race, it highlights the myriad issues researchers need to reflect upon before they attempt to make essentialist...of a true correlation.   It turned out there was indeed a bias, and it was coming through an unexpected...screens, they had also found the information they needed to begin correcting the issue. Working with James...

  18. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...vector performance. Yet, most of the technical data needed to replicate and build on others’ results is available... is a free resource to share this data and help speed scientific progress, but it will only be effective...Open Data Day with Addgene’s AAV Data Hub!Data Freedom: The Expansion of Data Sharing in Research Publications...

  19. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...terminals, which are jam packed with everything needed for synaptic transmission, are right around 250...Microscopy (EM) is one option, but the equipment needed for EM is not something you find in the average... don’t have access to the specialized equipment needed for EM or SRM? Enter Expansion Microscopy. What...

  20. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    ...does not drop significantly until protein levels exceed 70% of the sample content (Loughrey and Matlock...2019). To confirm RNA in your DNA sample, you will need to use a complementary assay, such as a fluorescence...readings are inherently more variable. If you do need to work with dilute samples:  Take multiple readings...
Showing: 381 - 400 of 829 results