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We narrowed to 629 results for: EMB

Showing: 381 - 400 of 629 results

  1. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...aliquoting purified and buffer exchanged virus, remember to make one or two small aliquots to use for qPCR...Tips When calculating the titer for your AAV, remember to account for the dilution of the sample for ...

  2. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ... adaptable into future magnetogenetic tools.  Embracing science in the open The York Lab operates with...applications across a wide range of topics. He embraces open science, self-publishing directly on his ...

  3. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...break), write down some notes on the card so you remember who they are and what you’ve talked about. After... Resources on the Addgene website Become of a member of the Addgene depositor community ...

  4. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...associate with the endogenously expressed SCF complex members, an E3 ubiquitin ligase complex. Finally, when ...Sep;11(9):713-20. doi: https://doi.org/10.1038/nchembio.1869  Nabet B, Roberts JM, Buckley DL, Paulk J...

  11. Tips for arabidopsis transformation

    Type
    Blog Post
    ...2007). Alternatively, if you prefer Golden Gate assembly, there are a series of plasmids from the Dinneny...Arabidopsis.html?id=IfZAMNPWVk4C&redir_esc=y (Accessed: 21 September 2018). Additional resources on the Addgene blog...

  12. An Introduction to Adenovirus

    Type
    Blog Post
    ... derived from the mastadenovirus members (mammalian-infecting members) of the Adenoviridae family, which...

  13. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...!). In 1997, Joseph Provost, then a new faculty member at Minnesota State (and now Professor of Chemistry...and it can be difficult to give up the control embedded in “cookbook” lab activities (follow the recipe...

  15. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...want to know the orientation of the insert. One member of this type of primer pair anneals to a sequence...Restriction Cloning Try SLIC cloning Learn about Gibson Assembly Additional Resources on Addgene.org Read about...

  16. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...nucleus. To circumvent this requirement for transmembrane proteins, a split ubiquitin system has been ...genetic system optimized for systematic studies of membrane protein interactions. Proc Natl Acad Sci U S A...

  17. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...technically gamma-retroviruses - another, separate member of the retroviral family. While both lentiviruses...Lentiviruses are able to enter an intact nuclear membrane allowing them to infect both dividing and non-...

  18. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...Maybe you’ll need to care for an elderly family member, want to train for a triathlon, finally buy that...laundry, take public transportation, call a cab and remember their own school assignments, the better. In my...

  19. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...Members of the bacterial world produce an assortment toxins to claim territory or kill competing microorganisms... (hok), a long-living toxin that damages cell membranes; Suppression of killing (sok), a short-lived RNA...
Showing: 381 - 400 of 629 results