We narrowed to 438 results for: ENA;

1. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

   Type

   Blog Post

   ...FLP-FRT, Cre-lox, and how combinations of FLP and Cre enable additional levels of genetic control. What is FLP...

2. Finding nucleic acids with SHERLOCK and DETECTR

   Type

   Blog Post

   ...amplification step in combination with the ssRNA reporter enables SHERLOCK to detect DNA or RNA with atomolar sensitivity...

3. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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   Blog Post

   ...PMC5424143. 2. Tsai, Shengdar Q., et al. "GUIDE-seq enables genome-wide profiling of off-target cleavage by...

4. Deciphering the Mysteries of Behavior with Viral Vectors

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   Blog Post

   ...be used as a readout for associated emotional phenomena like stress levels, but researchers have to be...

5. Save Time with Transient Plant Leaf Transformations

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   Blog Post

   ...cultures or plants, our transient expression method enables us to quickly screen and investigate transcription...

6. CRISPR Activation: A Practical Guide

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   Blog Post

   ...Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H,...

7. Tips for CRISPR Gene Editing in Mice

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   Blog Post

   ...that could be used to study a wide variety of phenomena, it’s possible that we’ll learn much more from...

8. Getting the Most from Your Lentiviral Transduction

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   Blog Post

   ...Majdoul, S., Martin, S., Merten, O.W., Galy, A., Fenard, D. ”Influence of mildly acidic pH conditions on...

9. Tips for Using FRET in Your Experiments

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   Blog Post

   ...Indeed, FRET was one of the first techniques which enabled the measurement of single molecule interactions...

10. Plasmids 101: Expressing shRNAs for RNA Interference

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    Blog Post

    ...target. RNAi is a valuable tool for your research arsenal, and there is still a dedicated field of study ...

11. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type

    Blog Post

    ...  Transfecting these three plasmids into cells enables the production of lentiviral vectors that are competent...

12. Antibodies 101: Beyond Surface Labeling

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    Blog Post

    ...while fluorescence-activated cell sorting (FACS) enables you to include further (non-lineage) markers alongside...

13. New Optogenetic Tools for Cytoskeleton and Membrane Control

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    Blog Post

    ...GTPase activity on a global to subcellular scale, enabling precise control over vascular endothelial barrier...

14. CRISPR 101: Homology Directed Repair

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    Blog Post

    ... the repair of breaks that lack a second end to enable DSBR or SDSA.   Homology directed repair and genome...

15. Transferable Skills Guide: Conflict Resolution

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    Blog Post

    ...nobody is really owning. Conflict in this latter scenario usually manifests as the assumption “I thought...

16. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type

    Blog Post

    ...intensity of the fluorescent signal is boosted and enables single molecule tracking in living cells. The HA...

17. The Fluorescent Vegetables in Aptamer Soup

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    Blog Post

    ...PubMed Central  PMCID: PMC5811451. Dolgosheina, Elena V., et al. "RNA mango aptamer-fluorophore: a bright...

18. Plasmids 101: Biotinylation

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    Blog Post

    ...determined to be due to a deficiency in Vitamin H later renamed Vitamin B7/biotin. Egg white injury would plague...

19. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type

    Blog Post

    ...compared to wild type BirA. TurboID and miniTurbo both enable biotin labeling in 10 minutes without any issues...

20. Fluorescent Proteins 101: When GFP lets you down

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    Blog Post

    ...module that can report on location. This approach enables the study of proteins in cells, but not other biomolecules...