We narrowed to 711 results for: ESC

1. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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   Blog Post

   ...BAC DNA can be modified with any of the below described approaches.    Bacterial strain with integrated...recombination system for chromosome engineering in Escherichia coli. PNAS 2000 97 (11) 5978-5983; May 16, 2000...

2. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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   ...step by step protocol for the csRNP method is described in our recent article (Dewari et al. 2018), however...tagging in human cells Plasmids 101: Protein Tags Fluorescent tagging of endogenous genes with SapTrap Resources...

3. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

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   Blog Post

   ...There are 8 major categories found in the table, describing everything from basic to advanced functionality...Software design elements that fall into this category describe how the user is expected to interact with the ...

4. Delivery Methods for Generating iPSCs

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   ...in practice integration can occur. Okita et al describes a protocol for generating iPSCs by plasmid transfection...transduction, such as the polyarginine peptide described in Zhou et al and Kim et al. This is considered...

5. CRISPR/Cas9 FAQs Answered!

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   Blog Post

   ...nickase system is based on the Cas9 D10A nickase described in Figure 4 of the Cong et al. (2013) Science ...co-transfect in HR donor. Alternatively, a new system described recently is the double nickase system, with PX335...

6. CRISPR 101: Cytosine and Adenine Base Editors

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   ...without introducing DSBs at all. Base editing to the rescue! Base editors chemically modify DNA bases without...named ABEmax. In 2020, two papers were published describing additional ABEs evolved from ABE7.10 that have...

7. Viral Vectors 101: Systemic Capsids

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   ... as well as the type of transgene delivered: fluorescent proteins, optogenetic sensors, CRISPR elements... Table 1 gives a brief overview of published descriptions of commonly used or emerging capsids. Selected...

8. CRISPR 101 eBook...en español

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   Blog Post

   ... de nuestra colección de libros electrónicos. Descargar CRISPR 101: Una herramienta de edición Addgene...

9. Overwhelmed? Take a Break With Our 5 Favorite Science Comics

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   Blog Post

   ...decreased immunological markers of stress in adolescents. It also put those young people in a better mood...

10. 2021 Deck the Lab Winners!

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    Blog Post

    ... Team at the Centre.     Lab support team @JohnInnesCentre rule pic.twitter.com/WMb97fpUOI— Sam Mugford...

11. Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes

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    Blog Post

    ...Security, pursuant to the international controls prescribed by the Australia Group, for these plasmids for...

12. Expanded Access to Rabies Plasmids

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    ...institution. Requesters will need to provide a brief description of the applicable research project when purchasing...

13. Top Requested Plasmid of 2017 - pMD2.G

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    Blog Post

    ... of the secretome, ubiquitin ligase, cellular senescence, autophagy, CRISPR, and so much more (find the...

14. Twelve Amazing Guest Blog Contributors!

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    ...from Promega, who wrote a guide to selecting fluorescent dyes and ligands. Illuminating Choices Paul...

15. Introducing Addgene's Microbiology Pages

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    ...We have Plasmid Kits for microbial work with fluorescent proteins, synthetic biology, gene cloning and...

16. 5 Science Rap Videos & Their Creator: Tom McFadden

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    ...One of Tom's earliest and most popular videos. Describes fossil rocks and their importance in understanding...

17. Call for Guest Bloggers

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    ... a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells   ...and many more...

18. An Interview with Connie Cepko - Gene Therapy, Plasmid Tools and Insights to Success

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    ... C.Y., et al. “A nanobody-based system using fluorescent proteins as scaffolds for cell-specific gene ...

19. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

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    Blog Post

    ... - it's all in the family. 3:56 - 7:04 : Alex describes the MD PhD process and explains why he loved the...

20. Podcast: Interview with Addgene's Founders

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    Blog Post

    ...Virus? Why Now? Resources at Addgene.org Find Fluorescent Protein Plasmids Find Plasmids for FRET Find ...