We narrowed to 444 results for: HAL;

1. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   ...also theoretically possible, but likely more challenging, with lentiviral expression. For more detail ...

2. A Guide to Starting Your Own Journal Club

   Type

   Blog Post

   ...success” of the journal club. This can be a challengingly vague metric but there are a few things you ...

3. SciComm: Taking Science to Elementary and Middle Schools

   Type

   Blog Post

   ...much in the making. One of the exciting, and challenging, aspects of talking to 8th graders was the broad...

4. Transferable Skills Guide: Identifying Your Transferable Skills

   Type

   Blog Post

   ...presents itself even outside of work, consider challenging yourself, take the opportunity, and discover ...

5. Tips for arabidopsis transformation

   Type

   Blog Post

   ... method of identifying transformed Arabidopsis thaliana seedlings following floral dip transformation’...

6. Simplify Cloning with in vivo Assembly

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   Blog Post

   ... to amplify away from the insertion site. Encode half the new sequence at the 5’ end of each primer, with...

7. AAVs in Retinal Gene Therapy

   Type

   Blog Post

   ... completing her PhD and medical training in Ophthalmology. Gene therapy was in its infancy, but the eye...

8. An Introduction to Adenovirus

   Type

   Blog Post

   ... like other viral vectors, though significant challenges remain (Wold & Toth, 2013).  While the pre-existing...

9. Better Dyeing Through Chemistry & Small Molecule Fluorophores

   Type

   Blog Post

   ... include: Self-labeling tags (e.g., SNAP-tag, HaloTag, TMP-tag) – These widely used systems consist of...

10. Tips for Screening with Yeast Two Hybrid Systems

    Type

    Blog Post

    ...functional luciferase only when fusions of the two halves of the luciferase protein interact and enable intein...

11. A Career in Grants: How To Become a Grant Professional

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    Blog Post

    ...Becoming a grant professional is rewarding and challenging!  Jo Miller, GPC, CSMS is the founder and principal...

12. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post

    ...and when they come into close proximity, the two halves of EGFP undergo folding, maturation, and fluorescence...

13. Twenty Years of Sharing: Expanding Accessibility

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    Blog Post

    ...persisted despite our best efforts. With a little over half of our orders coming from researchers outside the...

14. Hot Plasmids: Fall 2024

    Type

    Blog Post

    ... Zhong, B. L., Elliot, J. M., Wang, P., Li, H., Hall, R. N., Wang, B., Prakash, M., & Dunn, A. R. (2024...

15. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type

    Blog Post

    ...depending on their serotype, are particularly challenging. Their efficacy in any given system can be affected...

16. New CRISPR Tools: Cas7-11 and PASTE

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    Blog Post

    ...right integrase for this new approach proved a challenge. The old standby of CRE/flp didn’t work – it turns...

17. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    Blog Post

    ...WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang...

18. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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    Blog Post

    ...was recognized by meeting organizers in their town hall.  Iterative improvement - Making Binning Singletons...

19. Fluorescent Proteins 101: Aptamer Fluorophores

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    Blog Post

    ...technology has been studied and used in assays for over half a century; the Addgene blog has an excellent primer...

20. How to Write a Scientific Review Article

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    Blog Post

    ...Overall, writing a review can be overwhelming and challenging. My best advice is don’t overthink it. And to...