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We narrowed to 808 results for: MAK

Showing: 381 - 400 of 808 results

  2. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ... always excited when depositors find new ways to make this workhorse protein even more useful! From FPs...retain normal neuronal function, this system may make optogenetics in specific cell populations even easier...applications, including optogenetics, and they should make it much easier to conduct functional studies in ...

  3. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...plasmid such as pX330. This cloning strategy not only makes it easy to create a single gRNA-expressing plasmid...clone up to 7 gRNAs into one destination vector, making multiplexing easy.  Disadvantages of Golden Gate...TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt...

  4. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    ...proteins had to be re-engineered for each gene, making wide-scale gene activation seem next to impossible...activation compared to other CRISPR activators, making it a popular method for gene activation experiments...design (SunTag). This streamlines its delivery, making it a common choice for CRISPR activation. Newer...

  5. A Look at Addgene's QC Process

    Type
    Blog Post
    ...biological. Even the most high-fidelity polymerases can make mistakes and lead to the accumulation of errors ...ensure the entire team is using the same rubric and making the same decisions when analyzing the wide variety...the QC team leaves a note on the plasmid page to make requesting scientists aware of the identified discrepancy...

  6. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...spatiotemporal control or an “on/off switch.” This makes them great tools for providing a global overview... of the bait protein will be biotinylated. This makes it’s impossible to distinguish proteins that directly... proteins. This extended incubation period could make it difficult to detect proteins with short half-...

  7. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J,...plasmids from this publication at Addgene. 4. Makarova KS, Wolf YI, Alkhnbashi OS, Costa F, Shah SA, ...

  8. Engaging with science and society at pgEd

    Type
    Blog Post
    ...crucial if we want to make sure that everyone can be informed and empowered to make their own decisions ...posts, lesson plans, or information briefs for policymakers. The rapidly changing landscape of genetics ...

  9. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...Requiring an additional plasmid for transfection can make these optimization experiments a bit more challenging...experimental volumes accordingly. While you may need to make some final adjustments, this is generally a good...additional resources. References Kumar M, Keller B, Makalou N, Sutton RE. Systematic determination of the packaging...

  10. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...vehicles for genetic information. Therefore they make excellent tools for genetic engineering. There are...vectors when used in experiments. Retroviral vectors make up a large toolbox used by researchers for (among...shRNA or miRNA. The ever expanding CRISPR field makes wide use of retroviral delivery systems, especially...

  11. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...precipitates that permanently stain the membrane, making that section of the blot unusable for reprobing... if the mild is not effective. You may choose to make your own stripping solution using a recipe like ... Assess the stripping Now it’s time to check to make sure the antibodies from your first round of detection...

  12. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...it also helps you to hone your writing skills and make a name for yourself. Pick a topic that is close ...’t you record a podcast or put some effort into making a video? Use your unique talent to communicate ...Since then, I have tried many things: photography, making videos, presenting in pubs and at science festivals...

  13. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    ...meeting in person is often much more productive and makes it easier to build relationships. Nonetheless, the...responsibilities Being a leader in an organization, you need to make sure the organization speaks in “one voice” whilst...such as leadership skills can only assist you and make you more qualified for future career opportunities...

  14. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    ... who keep changing their minds and have trouble making decisions. In my work as a thesis coach for graduate...be reviewed, bring them to the meeting. This will make it easy for your supervisor to support you. 3) Explain...articles on our blog Read Joanne Kamen's Post on What Makes a Good Mentor Resources on the Addgene Wesbite ...

  15. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...m going to share what I’ve learned and hopefully make your decision a little easier if an industry postdoc... your goal is to enter academia, it is easier to make that transition from an academic setting—especially...company you choose. If publishing is important to you, make sure that your project will not involve proprietary...

  16. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ... techniques like super-resolution microscopy. To make this segment of the color spectrum more powerful... https://doi.org/10.1038/s41477-025-01989-9.   Make a splash with new LARRY barcoding libraries By Mike... can transduce neurons for retrograde labeling, making it a powerful tool for circuit tracing and other...

  17. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...capacity and stable expression of lentiviral vectors make them an attractive option. However, safety requirements...has psuedotypes. Psuedotyping is the process of making a hybrid AAV, with the genome from one serotype...Klingler, E., Kuhn, M., Farouni, R., Lagler, S., Kalamakis, G., Lischetti, U., Jabaudon, D., & Platt, R. ...

  18. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...usurped old methods or complemented them in ways that make them much easier. A special class of FPs, the FP...functionality with a single gene Although it is possible to make a timer system composed of two separate FPs (one... This one protein method simplifies cloning and makes your experimental setup less cumbersome. 3. Portability...

  19. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...Knockout/Knock-in plasmids Gene targeting methods also make it possible to insert, or knock-in, any gene, tag...instead want to create a conditional knockout. To make a conditional knockout, researchers often use the...floxed alleles. Although CRISPR is very good at making knockouts, knocking in large sections of DNA can...

  20. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    ...Addgene is accelerating this research by helping to make new cancer resources easily accessible to the research...found across all TCGA tumor samples is represented, making the results of any such study as clinically relevant...Addgene's cancer collection pages Scientists are making progress. New resources and technologies become...
Showing: 381 - 400 of 808 results