RANbodies: Reporter Nanobody Fusions
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Blog Post
...with an anti-HA antibody and serves as a backup means of detection that’s independent of the reporter....
We narrowed to 525 results for: MEA
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Visualizing Protein Turnover In Situ
TypeBlog Post...I was studying a synaptic protein, I wanted to measure it’s levels in its normal neuronal context. In ... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog Post... known as the resting potential (Vmem), can be measured as the imbalance of electrical charge between ... -
Plasmid Tools for Microbiome Studies
TypeBlog Post...generations can be determined. The lab used DCDC to measure growth rate of E. coli for over 10 generations ... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post...the cell membrane where cell voltage can then be measured. Archon1 is bright, exhibits good localization... -
Reproducibility for Everyone: Lessons from an Open Science Collaboration
TypeBlog Post...reproducibility workshops and organizational skills. Meanwhile, Addgene was also thinking about ways to address... -
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog Post...cell-adhesion proteins) targeting different organs. This means that exosomes not only promote microenvironment’... -
Antibodies 101: Conjugation
TypeBlog Post...2017) Simultaneous epitope and transcriptome measurement in single cells. Nat Methods 14:865–868. https... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog Post...Hubbard will image these neurons using GCaMP7, measuring calcium signaling related fluorescence during ... -
RNA Extraction Without A Kit
TypeBlog Post...Chirgwin et al., 1979). They developed a protocol meant for isolating RNA from rat spleens in which they... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog Post...Carrasco, and Markus W. Covert. "High-Sensitivity Measurements of Multiple Kinase Activities in Live Single... -
CRISPRainbow and Genome Visualization
TypeBlog Post...image represents the composite of fluorescent measurements from red, blue, and green channels overlaid ... -
Developing Lab Management Software for Biology
TypeBlog Post...Apache, and MariaDB. Using tried-and-true tools means less time getting up and running and more time working... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog Post... and arbitrary decision. Unfortunately, it also meant that, by the very nature of the way the experiments... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog Post...biological systems. The CombiGEM platform provides a means to create barcoded gRNA libraries that can be used... -
Addgene's Tips for Plasmid Quality Control
TypeBlog Post...opposed to purified DNA or colony sequencing. This means that we are sequencing from the exact same stock... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog Post...specifc, easily read sequences that can be used to measure their clonal abundance before and after a treatment... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog Post...very large number of regulators. That basically means larger genetic circuits that could conceivably be... -
Career Coaching for Scientists: Why and Where Do I Find One?
TypeBlog Post...transition out of the academic sphere into a different meaningful career in science. Supervisors in the academic... -
Design Tips for Prime Editing
TypeBlog Post...updated our blog post on Prime Editing, and that meant rereading many of the original papers reporting ...