We narrowed to 766 results for: Ncl

...for a successful grant application. It typically includes overview information, key dates, funding description...deliver outsider opinions, and reveal items to include for the next application. Although some comments...

...of the Retroviridae or Retrovirus family, which includes the gamma-retrovirus and lentivirus genera, have... field of mouse transgenics. Experimental uses include: Cre-dependent gene expression, gene knockout, ...

...Arg-mutant proteins in living baker’s yeast cells, including Arg-mutant yeast homologs of human proteins that...frequently found in genetic testing for syndromes that include developmental delay, result in loss of function...

...Various soCoChR plasmids described in this work including the soCoChR-GFP plasmid as well as several derivatives...expresses N-demethylases and other required enzymes, including a glutathione S-transferase homolog from a Janthinobacterium...

...sequences in DNA resulting in a variety of outcomes including excision or insertion, inversion, translocation...Lab constructs targeting GABA neurons. Serotypes include AAV1, and AAV9. Find viral preps here pAAV-hSyn-Cre-P2A-dTomato...

... species of bacteria.  A note on anti-CRISPR nomenclature Acr family proteins are named for the type of...from pathogenic bacteria’s CRISPR-Cas system. Conclusion CRISPR is a great way to make those genome edits...

...widespread use of FRET with fluorescent molecules, including fluorescent proteins, has led to the alternative...measuring the change in FRET efficiency, from which conclusions regarding the proximity of the two proteins can...

... infiltrate cells. Options for CRISPR delivery include plasmid transfection, RNP electroporation, and ...can also carry different types of CRISPR cargo including RNPs for indel formation via NHEJ, precise modification...

...order to see an effect. Hemizygous mice can be included in breeding strategies identical to those used...with transgenic mice that can impact reproduction include embryonic lethality, a short lifespan, or infertility...

...antibodies, we have developed a criterion that includes accuracy, strength, and reproducibility. We test... negative control to a sample with the primary included to confirm that the signal is from the primary...

...Standard Curve To do that calculation, you’ll need to include a standard curve in your experiment. These are ...are wells on the same ELISA plate that include known concentrations of your protein of interest. The optical...

...destroy. One treatment currently in clinical trials includes Cas3 (the shredder member of the Cas family) as... Other treatments currently in clinical trials include, but are not limited to, ones targeting muscular...

...plasmids! This category includes genes that help bacteria invade hosts, including attaching to host cells...

...plasmids use Rho-independent terminators, which include naturally occurring terminators, such as T7 and...mammalian terminators (SV40, hGH, BGH, and rbGlob) include the sequence motif AAUAAA which promotes both polyadenylation...

...quantification tend to be overestimates since they can include free p24 and defective viral particles. Direct ...infectious virus in a prep as the measurement will include defective particles. Studies have found that values...

...biologists working together on a variety of projects including the OpenWorm project. The main goal of OpenWorm... track them. We have a number of microscopes, including an automated microscope but we need to develop...

...gRNAGFP. Gonçalves says that advantages of AdVs include their episomal nature and very efficient introduction...using the Gonçalves and Ventura labs’ plasmids, including protocols, check out the plasmids at Addgene: ...

...standard quality control assays for AAVs. These steps include measuring viral titer and assessing purity. But...also conducting more rigorous quality control, including next generation sequencing (NGS) of the viral ...

... maintaining stable lines of genetic mutants, including in situations where maintaining heterozygosity..., they have fly collections for in vivo work, including CRISPR tools. The Vienna Drosophila Resource...

...used for colocalization analysis. Some examples include the ImageJ plugins JACoP and Coloc 2, BioImageXD...to the properties of FPs themselves. These can include the potential to oligomerize, protein charge, and...