We narrowed to 1,017 results for: PLE

...Controlling multiple genes with Gal4/UAS system One can also use Gal4 to drive expression of multiple genes ...the Gal4/UAS system. This incredibly useful, yet simple system allows you to systematically study gene ...downstream of a tissue-specific promoter sounds simpler, this modular system saves you from having to create... making a fly line in the next blog post). For example, the hedgehog gene can be overexpressed in several...under the control of UAS enhancer sequences. For example, if you discovered five new genes and wanted to...control purposes that there are dosage effects. For example, overexpression of two genes by one Gal4 driver...

...understanding these complex processes can’t be overstated, we can also learn a lot from Nature’s simpler solutions...engineer it to suit our purposes? In search of simple biological machines R bodies can repeatedly ...These questions can be addressed using R bodies: simple, force-generating protein machines that can extend...could be used in a variety of applications. For example, you could use them to lyse cells (and surrounding...

...time for that and you won’t be as productive. For example, the many resources available on the Addgene website...less dependent on the manager to succeed. More people in leadership roles leads to more ideas, initiative...enough to do…you are not delegating enough. For example, if we need to develop a new plasmid collection...duplicated. With each component of the project completed by an individual who is a local expert in that...result is high quality and the entire process is completed more efficiently. Listen to our podcast interview... Don’t give out tasks – where possible, include people in deciding what is to be delegated to them. Not...your time and skills. It also ensures that other people in the team grow and develop to reach their full...

... to determine precisely how the methods were implemented. We run into this problem frequently over the...not robust. Sampling noise There is, of course, simple noise in the process of sampling and inference-...problems above: unclear methods, QRPs, and small samples, then when replications do not come out the same...statistical tools that generate a p-value.  As an example, if you hypothesize that growth rates will be higher...answer those questions, the article is given “in-principle acceptance” to publish the final results regardless... special emphasis on positive controls and good sample sizes, concerns that are too easy to overlook when... worked with citizen scientists to design and implement authentic scientific research and as the Director...

... we've heard over and over from our users: "Please, please improve your plasmid maps!" After thoughtful...just how much things have improved, check out the example below. Our old map is on the left while the SnapGene...on to learn more about the improvements we’ve implemented and how they’ll make it easier for you to find... next cloning experiment early on. Beyond these simple but powerful display improvements, when you click...or ORF to learn more information about it. For example, if you hover over the ampicillin resistance gene...software development teams for working tirelessly to implement these improvements. Let us know what you think...

...Chemical agents, temperature, and light are all examples of factors that can lead to the induction of a... these three types of inducible promoters, and examples of each type. Many of these promoter systems are...transcription to begin. In addition to arabinose, cAMP complexed with cAMP activator protein (CAP) can also stimulate...stimulate AraC binding to I1 and I2 sites. Supplementing cell growth media with glucose decreases cAMP ...represses pBad, decreasing promoter leakiness.   Other examples of chemically induced promoters include positive...in plant research. Promoter Subtype Promoter Example Activator Addgene Plasmid/Kit Alcohol-regulated...temperatures but can be induced by heat or cold exposure. Examples include the heat shock-inducible Hsp70 or Hsp90...

...being one of the five senses? GPCRs or G-protein-coupled receptors (GPCRs)! Aside from these three senses...cascades.  So let’s break down the name "G-protein coupled receptor" into two parts: the receptor itself and...Takeda et al., 2002). G proteins: The receptor is coupled with heterotrimeric G proteins on the intracellular...binding of a new inactive heterotrimeric G protein complex and subsequently another round of signaling.    ...finally, to a response? Let’s take a look at a few examples with plasmids deposited at Addgene.  PRESTO-TANGO...gamma subunits of the heterotrimeric G protein complex that can be used to detect G protein activation...H, Mitaku S (2002) Identification of G protein-coupled receptor genes from the human genome sequence. ...

...). Mycoplasmas are considered to be one of the simplest and smallest bacteria. The absence of a rigid ...techniques. These include reusing pipettes for multiple cell lines rather than using disposable ones or... of powdered media. Filtration does not ensure complete sterilization as mycoplasma can even escape 0.2... growth of mycoplasma but cannot eradicate it completely. When mycoplasmas are seen in the culture, it...by providing proper training and guidance to the people working in the lab facility. This can be done by...mycoplasma infection from one cell line to another. Multiple cell lines should not be stored in a single incubator...cultures. The treatment can take a few weeks to a couple of months depending on the severity of the infection...

... short science policy career and this seemingly simple question is a moving target. There are a multitude...pandemic has foisted upon us. Action Items:  A simple place to look for science policy folks is on Twitter... data gathering, regulatory efforts, policy implementation, program management, communications, diplomacy...setting: drafting legislation, making rules, and implementing the policy within the jurisdiction of the governing...working in science policy doesn’t have to be a complete career pivot. There are many ways to pursue your...your interests in policy or advocacy as a supplement to your career through engagement with your local...proposals. Check out the Day One Project for some example proposals and consider applying for their accelerator...

...disease estimated to affect 0.27 to 4.6 per 10,000 people globally. Pustules form from the uncontrolled activation...where one of the X chromosomes is partially or completely missing in women. Bicuspid aortic valve (an aortic... mutations affect signaling pathways G protein-coupled receptors (GPCRs) detect molecules outside of the...translate those signals into cellular responses. For example, GPCRs and their downstream G protein partners ...with an emphasis on oligomerization, signalling complex assembly, and localization to the nucleus,” says...at McGill University.  These protein-receptor complexes consist of many parts that work together on the...gene, which codes for G𝛃1 subunits of G protein complexes, lead to a rare type of developmental delay, and...

...recent advances have allowed scientists to track multiple genomic loci over time using all the colors of...modified GFP-dCas9 and the gRNA scaffold to enhance complex assembly, thereby decreasing the amount of background...at a range of distances from 2-75 Mbp. Labeling multiple, intrachromosomal loci Building on the work of...al., Thoru Pederson’s lab used CRISPR to label multiple loci in distinct colors. To create a colorful ... Ma et al. performed sophisticated tracking of multiple chromosomal loci in live cells. However, they ...additional hairpin/fluorescent protein combination, for example, with a far-red FP, would increase the available...publication at Addgene. 3. Ma, Hanhui, et al. “Multiplexed labeling of genomic loci with dCas9 and engineered...

...’s scientific seminars a couple days a week. And there there’s always plenty of writing: plain-language...social behaviour in social insects, obviously as all people are,” says Susan. Her passion for insects lead ...show. After graduating with a master’s, Susan completed a science writing internship with the National...planning.” To beef up her resume, she decided to complete a certificate in Strategic Communications and ...the most satisfying parts of the job is having completed one of these difficult pieces: “It’s probably ...starting a blog, but with one caveat: “I hate telling people to work for free because you shouldn’t work for...

...coaches will have loads of success stories and plenty of people to vouch for them. Trust your gut – only...I have seen, without exception, that this helps people do a better job at reaching their goals.  Check...costs. Can you suggest some coaches? Yes I can. Please note that Addgene does not endorse any of these...Sarah Cardozo Duncan is a proven coach who helps people in science and tech get jobs. She is also a very...? Some coaches focus on graduate students. For example Dora Farkas, founder of Finish Your Thesis (who...based in Spain. The bottom line – there are lots of people out there who want to help. Don’t be afraid to ...

...process you would like to monitor? Neurons, for example, have unusually fast calcium dynamics, whereas ... is most sensitive to your expected range. For example, calcium in the ER lumen reaches the sub-millimolar...binding of the biosensor to endogenous calmodulin. Multiplexing Are you combining your calcium reading with ...you need to free up crowded optical space for multiplexing, GECO sensors are available in green, blue, ...Additional red sensors based on mRuby (jRCaMP1) and mApple (jRGECO1a) are also available. Detection methods...single fluorescent protein (FP), requiring only a simple measurement. However, single wavelength measurements...Genetically encoded calcium indicators to probe complex brain circuit dynamics in vivo. Neuroscience Research...

...In order to understand the complex wiring of the brain and the underpinnings of neurological disease,...while resulting in minimal off target effects. For example, if your receptors are expressed in the brain, ...exclusively activated by designer drugs, are G protein-coupled receptors (GPCRs), that have been reprogrammed ...receptor acts through. The DREADD hM3D(Gq), for example, lowers the threshold for neuronal signaling and...themselves are modified ligand binding domains. When coupled to the ion pore domain (IPD) of a channel, the ...expression and signaling of a specifically designed Gi-coupled receptor in transgenic mice. Nature Biotechnology... Multiple Authors...

...task, action, and results. While this method seems simple, I found it hard to remember to describe all four... more than one theme and can be used to answer multiple types of questions. Writing a rough outline of... say You’re going to be interviewed by numerous people during your industry interview. Each interview ...interview. Expect to be tired once the interview is complete Industry interviews are long! Mine lasted 4.5 ...interviewer and then thank them once the interview is complete. Remember, your interviewers took time out of ...remotely Remote interviews are likely new to most people - even hiring managers and your interviewers. You...a question. Maybe you ask for questions after completing each section of your talk. Some video conferencing...

... or Cas nickase)  a guide RNA that targets the complex of TadA and dCas to the genomic DNA of interest...effect of removing the wild-type TadA from the complex the researchers developed a second set: The ABE8... the team replaced S. pyogenes Cas9 in the ABE complex with PAM-variant Cas9 proteins: the S. pyogenes... ability to use non-standard Cas9 in the ABE8 complexes enables a broader targeting scope for base editing... the corrected cells back to the patients. For example, defects in hemoglobin (𝛽-globin), which cause... is very useful, sometimes, you need to modify multiple genes to generate a cellular phenotype. Again ...that require high DNA editing specificity, for example for potential therapeutic approaches, the authors...

...particles = number of particles per mL of sample. The purity of the sample can be estimated by looking at the...negligible toxicity. However, their production is more complex and the presence of helper virus contaminants have...final recombinant adenoviral plasmid construct, please see Addgene’s Adenoviral Guide webpage, and references...which time the cells are scraped, and lysed by multiple freeze-thaw cycles. The resultant lysate is your...amplification.*Pro-Tip*: The cells will become completely confluent and the media will turn yellow. Do ...cytopathic effect.” Amplification can be repeated multiple times (2-4 rounds) at increasing scale over the...

...high and above what would be expected in patient samples (e.g.: blood, urine). Adapting toehold switches...of the emerging outbreak, as many as 2.2 billion people globally live in areas conducive to the spread ...involved definitely contributed to our ability to complete the project on such a short timescale. Our article...pathogen’s genome as unique barcodes to identify samples containing the virus. Of the 48 sensors, targeting... diagnostic results.  Our approach now detects samples at clinically relevant concentrations (1-3 fM; ... look at viral load in patient blood and urine samples; we showed that we can detect the virus directly...Interview with Previous Collin's Lab Member and Sample 6 Cofounder Michael Koeris Learn How Minigenomes...

...structure of the hairpin-DNA complex is thought to destabilize the transcription complex, initiating cleavage ...Polyadenylation is the post-transcriptional additional of multiple adenine (A) nucleotides to the tail of a messenger...polymerase terminates differently. Polymerase III, for example, relies on a specific sequence and RNA secondary...mechanism, whereas Polymerase II termination is more complex and involves two RNA polymerase-associated proteins...polyadenylation enzymes, in a process that seems to couple termination with polyadenlyation. Mammalian expression...PMC104046. Wodrich H, Schambach A, Kräusslich H-G. Multiple copies of the Mason–Pfizer monkey virus constitutive...